Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05345 and BWI76_RS05350 overlap by 4 nucleotides BWI76_RS05350 and BWI76_RS05355 are separated by 108 nucleotides BWI76_RS05355 and BWI76_RS05360 are separated by 39 nucleotides BWI76_RS05360 and BWI76_RS05365 overlap by 8 nucleotides
BWI76_RS05345: BWI76_RS05345 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,125,929 to 1,126,636
_RS05345
BWI76_RS05350: BWI76_RS05350 - Rcs stress response system protein RcsF, at 1,126,633 to 1,127,040
_RS05350
BWI76_RS05355: BWI76_RS05355 - methionine ABC transporter substrate-binding protein MetQ, at 1,127,149 to 1,127,964
_RS05355
BWI76_RS05360: BWI76_RS05360 - methionine ABC transporter permease, at 1,128,004 to 1,128,657
_RS05360
BWI76_RS05365: BWI76_RS05365 - D-methionine ABC transporter, ATP-binding protein, at 1,128,650 to 1,129,681
_RS05365
Position (kb) 1127
1128 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1126.227 kb on + strand, within BWI76_RS05345 at 1126.434 kb on + strand, within BWI76_RS05345 at 1126.487 kb on + strand, within BWI76_RS05345 at 1126.507 kb on + strand, within BWI76_RS05345 at 1126.515 kb on - strand, within BWI76_RS05345 at 1126.553 kb on - strand, within BWI76_RS05345 at 1126.555 kb on + strand, within BWI76_RS05345 at 1126.659 kb on + strand at 1126.677 kb on + strand, within BWI76_RS05350 at 1126.695 kb on - strand, within BWI76_RS05350 at 1126.751 kb on + strand, within BWI76_RS05350 at 1126.759 kb on - strand, within BWI76_RS05350 at 1126.954 kb on + strand, within BWI76_RS05350 at 1126.962 kb on - strand, within BWI76_RS05350 at 1126.979 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.989 kb on - strand, within BWI76_RS05350 at 1127.008 kb on - strand at 1127.138 kb on - strand at 1127.155 kb on + strand at 1127.163 kb on - strand at 1127.214 kb on + strand at 1127.221 kb on - strand at 1127.301 kb on - strand, within BWI76_RS05355 at 1127.301 kb on - strand, within BWI76_RS05355 at 1127.302 kb on - strand, within BWI76_RS05355 at 1127.542 kb on + strand, within BWI76_RS05355 at 1127.690 kb on + strand, within BWI76_RS05355 at 1127.693 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.892 kb on + strand at 1127.945 kb on - strand at 1128.075 kb on + strand, within BWI76_RS05360 at 1128.081 kb on + strand, within BWI76_RS05360 at 1128.089 kb on + strand, within BWI76_RS05360 at 1128.111 kb on + strand, within BWI76_RS05360 at 1128.206 kb on - strand, within BWI76_RS05360 at 1128.442 kb on - strand, within BWI76_RS05360 at 1128.442 kb on - strand, within BWI76_RS05360 at 1128.691 kb on - strand at 1128.753 kb on - strand at 1128.753 kb on - strand at 1128.898 kb on - strand, within BWI76_RS05365
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM remove 1,126,227 + BWI76_RS05345 0.42 +2.5 1,126,434 + BWI76_RS05345 0.71 +1.3 1,126,487 + BWI76_RS05345 0.79 +0.0 1,126,507 + BWI76_RS05345 0.82 -1.1 1,126,515 - BWI76_RS05345 0.83 +0.7 1,126,553 - BWI76_RS05345 0.88 -0.0 1,126,555 + BWI76_RS05345 0.88 +1.0 1,126,659 + -0.1 1,126,677 + BWI76_RS05350 0.11 -1.3 1,126,695 - BWI76_RS05350 0.15 -0.1 1,126,751 + BWI76_RS05350 0.29 -0.7 1,126,759 - BWI76_RS05350 0.31 +0.2 1,126,954 + BWI76_RS05350 0.79 -0.6 1,126,962 - BWI76_RS05350 0.81 -2.3 1,126,979 + BWI76_RS05350 0.85 -0.5 1,126,986 + BWI76_RS05350 0.87 -0.6 1,126,986 + BWI76_RS05350 0.87 -0.4 1,126,986 + BWI76_RS05350 0.87 +0.2 1,126,989 - BWI76_RS05350 0.87 -1.2 1,127,008 - -3.0 1,127,138 - -0.5 1,127,155 + -0.2 1,127,163 - +1.1 1,127,214 + -1.4 1,127,221 - +0.2 1,127,301 - BWI76_RS05355 0.19 -1.3 1,127,301 - BWI76_RS05355 0.19 +0.3 1,127,302 - BWI76_RS05355 0.19 -0.3 1,127,542 + BWI76_RS05355 0.48 -1.9 1,127,690 + BWI76_RS05355 0.66 +1.4 1,127,693 - BWI76_RS05355 0.67 -1.5 1,127,698 - BWI76_RS05355 0.67 -2.4 1,127,698 - BWI76_RS05355 0.67 -2.7 1,127,892 + -0.1 1,127,945 - -0.5 1,128,075 + BWI76_RS05360 0.11 -3.0 1,128,081 + BWI76_RS05360 0.12 +0.5 1,128,089 + BWI76_RS05360 0.13 -1.3 1,128,111 + BWI76_RS05360 0.16 +0.4 1,128,206 - BWI76_RS05360 0.31 -0.4 1,128,442 - BWI76_RS05360 0.67 -0.1 1,128,442 - BWI76_RS05360 0.67 -0.3 1,128,691 - -1.1 1,128,753 - +0.3 1,128,753 - -0.5 1,128,898 - BWI76_RS05365 0.24 -0.4
Or see this region's nucleotide sequence