Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00980 and BWI76_RS00985 are separated by 58 nucleotides BWI76_RS00985 and BWI76_RS00990 are separated by 163 nucleotides
BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691
_RS00980
BWI76_RS00985: BWI76_RS00985 - transcriptional regulator IlvY, at 201,750 to 202,640
_RS00985
BWI76_RS00990: BWI76_RS00990 - ketol-acid reductoisomerase, at 202,804 to 204,279
_RS00990
Position (kb) 201
202
203 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 200.792 kb on + strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.869 kb on - strand, within BWI76_RS00980 at 200.929 kb on + strand, within BWI76_RS00980 at 201.380 kb on - strand, within BWI76_RS00980 at 201.454 kb on - strand at 201.505 kb on + strand, within BWI76_RS00980 at 201.565 kb on - strand at 201.629 kb on - strand at 201.918 kb on - strand, within BWI76_RS00985 at 201.928 kb on - strand, within BWI76_RS00985 at 201.974 kb on + strand, within BWI76_RS00985 at 202.030 kb on - strand, within BWI76_RS00985 at 202.043 kb on - strand, within BWI76_RS00985 at 202.101 kb on - strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.114 kb on + strand, within BWI76_RS00985 at 202.177 kb on + strand, within BWI76_RS00985 at 202.264 kb on + strand, within BWI76_RS00985 at 202.444 kb on + strand, within BWI76_RS00985 at 202.515 kb on + strand, within BWI76_RS00985 at 202.560 kb on + strand at 202.610 kb on + strand at 202.625 kb on - strand at 202.796 kb on + strand at 202.862 kb on - strand at 202.960 kb on + strand, within BWI76_RS00990 at 202.968 kb on - strand, within BWI76_RS00990 at 202.971 kb on + strand, within BWI76_RS00990 at 202.976 kb on + strand, within BWI76_RS00990 at 203.048 kb on - strand, within BWI76_RS00990 at 203.182 kb on + strand, within BWI76_RS00990 at 203.379 kb on + strand, within BWI76_RS00990 at 203.617 kb on - strand, within BWI76_RS00990 at 203.617 kb on - strand, within BWI76_RS00990
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 20mM remove 200,792 + BWI76_RS00980 0.42 -2.2 200,845 - BWI76_RS00980 0.45 -0.2 200,845 - BWI76_RS00980 0.45 -3.3 200,869 - BWI76_RS00980 0.47 -4.0 200,929 + BWI76_RS00980 0.51 -5.0 201,380 - BWI76_RS00980 0.80 -1.4 201,454 - -0.3 201,505 + BWI76_RS00980 0.88 -3.5 201,565 - -4.3 201,629 - -2.2 201,918 - BWI76_RS00985 0.19 -1.5 201,928 - BWI76_RS00985 0.20 -3.1 201,974 + BWI76_RS00985 0.25 -1.5 202,030 - BWI76_RS00985 0.31 -4.3 202,043 - BWI76_RS00985 0.33 -3.7 202,101 - BWI76_RS00985 0.39 -3.7 202,114 + BWI76_RS00985 0.41 -3.3 202,114 + BWI76_RS00985 0.41 -4.1 202,177 + BWI76_RS00985 0.48 -4.5 202,264 + BWI76_RS00985 0.58 -3.2 202,444 + BWI76_RS00985 0.78 -1.2 202,515 + BWI76_RS00985 0.86 -2.6 202,560 + -2.1 202,610 + -1.2 202,625 - -3.6 202,796 + -2.8 202,862 - -4.4 202,960 + BWI76_RS00990 0.11 +2.2 202,968 - BWI76_RS00990 0.11 -5.8 202,971 + BWI76_RS00990 0.11 -5.2 202,976 + BWI76_RS00990 0.12 -5.1 203,048 - BWI76_RS00990 0.17 -2.1 203,182 + BWI76_RS00990 0.26 -5.5 203,379 + BWI76_RS00990 0.39 -3.7 203,617 - BWI76_RS00990 0.55 -0.8 203,617 - BWI76_RS00990 0.55 -4.5
Or see this region's nucleotide sequence