Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_549 and Pf6N2E2_550 are separated by 1 nucleotides Pf6N2E2_550 and Pf6N2E2_551 overlap by 37 nucleotides
Pf6N2E2_549 - ClpB protein, at 619,344 to 621,887
_549
Pf6N2E2_550 - Uncharacterized protein ImpH/VasB, at 621,889 to 622,914
_550
Pf6N2E2_551 - Protein ImpG/VasA, at 622,878 to 624,668
_551
Position (kb) 621
622
623 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 621.352 kb on + strand, within Pf6N2E2_549 at 621.353 kb on - strand, within Pf6N2E2_549 at 621.353 kb on - strand, within Pf6N2E2_549 at 621.450 kb on + strand, within Pf6N2E2_549 at 621.450 kb on + strand, within Pf6N2E2_549 at 621.450 kb on + strand, within Pf6N2E2_549 at 621.451 kb on - strand, within Pf6N2E2_549 at 621.582 kb on + strand, within Pf6N2E2_549 at 621.583 kb on - strand, within Pf6N2E2_549 at 621.793 kb on + strand at 621.793 kb on + strand at 621.794 kb on - strand at 622.061 kb on + strand, within Pf6N2E2_550 at 622.071 kb on - strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.520 kb on + strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.521 kb on - strand, within Pf6N2E2_550 at 622.620 kb on - strand, within Pf6N2E2_550 at 622.975 kb on + strand at 622.976 kb on - strand at 623.542 kb on + strand, within Pf6N2E2_551 at 623.575 kb on + strand, within Pf6N2E2_551 at 623.575 kb on + strand, within Pf6N2E2_551 at 623.576 kb on - strand, within Pf6N2E2_551 at 623.578 kb on + strand, within Pf6N2E2_551 at 623.815 kb on + strand, within Pf6N2E2_551 at 623.815 kb on + strand, within Pf6N2E2_551 at 623.815 kb on + strand, within Pf6N2E2_551 at 623.821 kb on + strand, within Pf6N2E2_551 at 623.821 kb on + strand, within Pf6N2E2_551 at 623.821 kb on + strand, within Pf6N2E2_551 at 623.896 kb on + strand, within Pf6N2E2_551 at 623.897 kb on - strand, within Pf6N2E2_551
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 621,352 + Pf6N2E2_549 0.79 -0.1 621,353 - Pf6N2E2_549 0.79 -1.8 621,353 - Pf6N2E2_549 0.79 -0.3 621,450 + Pf6N2E2_549 0.83 -0.1 621,450 + Pf6N2E2_549 0.83 +0.3 621,450 + Pf6N2E2_549 0.83 +1.4 621,451 - Pf6N2E2_549 0.83 -1.2 621,582 + Pf6N2E2_549 0.88 -4.0 621,583 - Pf6N2E2_549 0.88 +0.6 621,793 + +0.8 621,793 + +0.3 621,794 - -4.0 622,061 + Pf6N2E2_550 0.17 -3.3 622,071 - Pf6N2E2_550 0.18 +1.1 622,520 + Pf6N2E2_550 0.62 +0.5 622,520 + Pf6N2E2_550 0.62 +0.6 622,520 + Pf6N2E2_550 0.62 +1.1 622,521 - Pf6N2E2_550 0.62 +0.3 622,521 - Pf6N2E2_550 0.62 +1.4 622,521 - Pf6N2E2_550 0.62 +0.7 622,620 - Pf6N2E2_550 0.71 +1.3 622,975 + -1.2 622,976 - +1.0 623,542 + Pf6N2E2_551 0.37 +0.3 623,575 + Pf6N2E2_551 0.39 +1.0 623,575 + Pf6N2E2_551 0.39 -0.7 623,576 - Pf6N2E2_551 0.39 -0.1 623,578 + Pf6N2E2_551 0.39 +0.1 623,815 + Pf6N2E2_551 0.52 -0.8 623,815 + Pf6N2E2_551 0.52 -0.7 623,815 + Pf6N2E2_551 0.52 +0.3 623,821 + Pf6N2E2_551 0.53 +0.6 623,821 + Pf6N2E2_551 0.53 +0.4 623,821 + Pf6N2E2_551 0.53 +0.8 623,896 + Pf6N2E2_551 0.57 +1.0 623,897 - Pf6N2E2_551 0.57 -0.6
Or see this region's nucleotide sequence