Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1840 and Pf6N2E2_1841 are separated by 10 nucleotides Pf6N2E2_1841 and Pf6N2E2_1842 are separated by 179 nucleotides Pf6N2E2_1842 and Pf6N2E2_1843 are separated by 429 nucleotides
Pf6N2E2_1840 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,178,554 to 2,179,366
_1840
Pf6N2E2_1841 - hypothetical protein, at 2,179,377 to 2,179,526
_1841
Pf6N2E2_1842 - Transcriptional regulator, TetR family, at 2,179,706 to 2,180,350
_1842
Pf6N2E2_1843 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,180,780 to 2,181,541
_1843
Position (kb)
2179
2180
2181 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2178.707 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.105 kb on + strand, within Pf6N2E2_1840 at 2179.107 kb on + strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.142 kb on - strand, within Pf6N2E2_1840 at 2179.560 kb on + strand at 2179.566 kb on - strand at 2179.744 kb on + strand at 2179.773 kb on + strand, within Pf6N2E2_1842 at 2179.773 kb on + strand, within Pf6N2E2_1842 at 2179.774 kb on - strand, within Pf6N2E2_1842 at 2179.774 kb on - strand, within Pf6N2E2_1842 at 2179.996 kb on - strand, within Pf6N2E2_1842 at 2180.171 kb on - strand, within Pf6N2E2_1842 at 2180.171 kb on - strand, within Pf6N2E2_1842 at 2180.178 kb on - strand, within Pf6N2E2_1842 at 2180.224 kb on + strand, within Pf6N2E2_1842 at 2180.224 kb on + strand, within Pf6N2E2_1842 at 2180.225 kb on - strand, within Pf6N2E2_1842 at 2180.225 kb on - strand, within Pf6N2E2_1842 at 2180.241 kb on + strand, within Pf6N2E2_1842 at 2180.360 kb on - strand at 2180.678 kb on + strand at 2180.937 kb on + strand, within Pf6N2E2_1843 at 2181.173 kb on + strand, within Pf6N2E2_1843 at 2181.174 kb on - strand, within Pf6N2E2_1843 at 2181.206 kb on + strand, within Pf6N2E2_1843 at 2181.206 kb on + strand, within Pf6N2E2_1843 at 2181.206 kb on + strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.207 kb on - strand, within Pf6N2E2_1843 at 2181.219 kb on - strand, within Pf6N2E2_1843 at 2181.249 kb on - strand, within Pf6N2E2_1843
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 2,178,707 - Pf6N2E2_1840 0.19 +0.1 2,179,087 - Pf6N2E2_1840 0.66 -0.1 2,179,087 - Pf6N2E2_1840 0.66 +1.5 2,179,105 + Pf6N2E2_1840 0.68 -1.9 2,179,107 + Pf6N2E2_1840 0.68 +1.8 2,179,108 - Pf6N2E2_1840 0.68 -3.0 2,179,108 - Pf6N2E2_1840 0.68 -1.6 2,179,141 + Pf6N2E2_1840 0.72 +0.9 2,179,141 + Pf6N2E2_1840 0.72 +1.2 2,179,142 - Pf6N2E2_1840 0.72 -0.5 2,179,560 + +0.2 2,179,566 - +2.3 2,179,744 + -4.0 2,179,773 + Pf6N2E2_1842 0.10 -3.1 2,179,773 + Pf6N2E2_1842 0.10 -0.9 2,179,774 - Pf6N2E2_1842 0.11 +0.2 2,179,774 - Pf6N2E2_1842 0.11 +0.4 2,179,996 - Pf6N2E2_1842 0.45 -3.4 2,180,171 - Pf6N2E2_1842 0.72 -1.3 2,180,171 - Pf6N2E2_1842 0.72 +0.3 2,180,178 - Pf6N2E2_1842 0.73 -0.0 2,180,224 + Pf6N2E2_1842 0.80 +0.5 2,180,224 + Pf6N2E2_1842 0.80 -2.6 2,180,225 - Pf6N2E2_1842 0.80 +1.3 2,180,225 - Pf6N2E2_1842 0.80 -0.9 2,180,241 + Pf6N2E2_1842 0.83 -1.0 2,180,360 - -1.4 2,180,678 + +0.7 2,180,937 + Pf6N2E2_1843 0.21 -0.6 2,181,173 + Pf6N2E2_1843 0.52 +2.5 2,181,174 - Pf6N2E2_1843 0.52 -0.3 2,181,206 + Pf6N2E2_1843 0.56 -1.1 2,181,206 + Pf6N2E2_1843 0.56 -2.1 2,181,206 + Pf6N2E2_1843 0.56 +0.2 2,181,207 - Pf6N2E2_1843 0.56 -0.6 2,181,207 - Pf6N2E2_1843 0.56 +1.1 2,181,207 - Pf6N2E2_1843 0.56 +1.5 2,181,207 - Pf6N2E2_1843 0.56 +0.6 2,181,219 - Pf6N2E2_1843 0.58 +0.1 2,181,249 - Pf6N2E2_1843 0.62 +0.0
Or see this region's nucleotide sequence