Experiment: Glucuronamide (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1080 and Pf6N2E2_1081 are separated by 85 nucleotides Pf6N2E2_1081 and Pf6N2E2_1082 overlap by 1 nucleotides Pf6N2E2_1082 and Pf6N2E2_1083 are separated by 230 nucleotides Pf6N2E2_1083 and Pf6N2E2_1084 are separated by 150 nucleotides
Pf6N2E2_1080 - hydrolase, alpha/beta fold family, at 1,264,415 to 1,265,446
_1080
Pf6N2E2_1081 - probable membrane protein YPO3302, at 1,265,532 to 1,265,966
_1081
Pf6N2E2_1082 - CopG protein, at 1,265,966 to 1,266,412
_1082
Pf6N2E2_1083 - hypothetical protein, at 1,266,643 to 1,266,780
_1083
Pf6N2E2_1084 - hypothetical protein, at 1,266,931 to 1,267,119
_1084
Position (kb)
1265
1266 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1264.554 kb on - strand, within Pf6N2E2_1080 at 1264.560 kb on + strand, within Pf6N2E2_1080 at 1264.560 kb on + strand, within Pf6N2E2_1080 at 1264.674 kb on + strand, within Pf6N2E2_1080 at 1264.675 kb on - strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.701 kb on + strand, within Pf6N2E2_1080 at 1264.702 kb on - strand, within Pf6N2E2_1080 at 1264.893 kb on - strand at 1265.028 kb on - strand, within Pf6N2E2_1080 at 1265.089 kb on - strand, within Pf6N2E2_1080 at 1265.102 kb on - strand, within Pf6N2E2_1080 at 1265.263 kb on - strand, within Pf6N2E2_1080 at 1265.316 kb on + strand, within Pf6N2E2_1080 at 1265.317 kb on - strand, within Pf6N2E2_1080 at 1265.322 kb on + strand, within Pf6N2E2_1080 at 1265.350 kb on - strand at 1265.492 kb on - strand at 1265.710 kb on + strand, within Pf6N2E2_1081 at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.953 kb on + strand at 1265.954 kb on - strand at 1265.954 kb on - strand at 1266.043 kb on + strand, within Pf6N2E2_1082 at 1266.043 kb on + strand, within Pf6N2E2_1082 at 1266.044 kb on - strand, within Pf6N2E2_1082 at 1266.495 kb on - strand at 1266.587 kb on - strand at 1266.602 kb on + strand at 1266.602 kb on + strand at 1266.602 kb on + strand at 1266.644 kb on + strand at 1266.760 kb on + strand, within Pf6N2E2_1083 at 1266.760 kb on + strand, within Pf6N2E2_1083 at 1266.761 kb on - strand, within Pf6N2E2_1083 at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.780 kb on + strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.781 kb on - strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.784 kb on + strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.785 kb on - strand at 1266.819 kb on + strand at 1266.820 kb on - strand at 1266.835 kb on + strand at 1266.874 kb on - strand at 1266.912 kb on + strand at 1266.913 kb on - strand at 1266.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Glucuronamide (N) remove 1,264,554 - Pf6N2E2_1080 0.13 +0.1 1,264,560 + Pf6N2E2_1080 0.14 +1.0 1,264,560 + Pf6N2E2_1080 0.14 -0.2 1,264,674 + Pf6N2E2_1080 0.25 +0.9 1,264,675 - Pf6N2E2_1080 0.25 -1.0 1,264,701 + Pf6N2E2_1080 0.28 -3.4 1,264,701 + Pf6N2E2_1080 0.28 +1.1 1,264,701 + Pf6N2E2_1080 0.28 +0.4 1,264,702 - Pf6N2E2_1080 0.28 +0.8 1,264,893 - +0.9 1,265,028 - Pf6N2E2_1080 0.59 -1.4 1,265,089 - Pf6N2E2_1080 0.65 -0.3 1,265,102 - Pf6N2E2_1080 0.67 -3.1 1,265,263 - Pf6N2E2_1080 0.82 -2.7 1,265,316 + Pf6N2E2_1080 0.87 -0.6 1,265,317 - Pf6N2E2_1080 0.87 -2.0 1,265,322 + Pf6N2E2_1080 0.88 -2.6 1,265,350 - -0.7 1,265,492 - -0.2 1,265,710 + Pf6N2E2_1081 0.41 -0.8 1,265,953 + +0.4 1,265,953 + -2.6 1,265,953 + +0.6 1,265,953 + -0.9 1,265,953 + +1.0 1,265,954 - +0.4 1,265,954 - -0.3 1,266,043 + Pf6N2E2_1082 0.17 -0.0 1,266,043 + Pf6N2E2_1082 0.17 -2.3 1,266,044 - Pf6N2E2_1082 0.17 +1.5 1,266,495 - +0.1 1,266,587 - -2.0 1,266,602 + +2.4 1,266,602 + +2.0 1,266,602 + +1.3 1,266,644 + -3.0 1,266,760 + Pf6N2E2_1083 0.85 +2.8 1,266,760 + Pf6N2E2_1083 0.85 -0.1 1,266,761 - Pf6N2E2_1083 0.86 +0.7 1,266,780 + +0.6 1,266,780 + +1.4 1,266,780 + +0.8 1,266,780 + -0.4 1,266,780 + +0.2 1,266,780 + +1.9 1,266,781 - +0.2 1,266,781 - -0.9 1,266,781 - -0.2 1,266,781 - -1.2 1,266,781 - +1.0 1,266,784 + -0.1 1,266,784 + +1.6 1,266,784 + +0.9 1,266,784 + +1.0 1,266,784 + +0.1 1,266,785 - +1.3 1,266,785 - -1.3 1,266,785 - +0.2 1,266,785 - +0.6 1,266,785 - +1.1 1,266,785 - -3.0 1,266,785 - -0.7 1,266,785 - +0.4 1,266,785 - +0.0 1,266,819 + -1.6 1,266,820 - -0.9 1,266,835 + +1.1 1,266,874 - -1.1 1,266,912 + -0.2 1,266,913 - -1.5 1,266,929 + -2.0
Or see this region's nucleotide sequence