Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23270

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23265 and BWI76_RS23270 are separated by 227 nucleotidesBWI76_RS23270 and BWI76_RS23275 are separated by 12 nucleotidesBWI76_RS23275 and BWI76_RS23280 are separated by 39 nucleotidesBWI76_RS23280 and BWI76_RS23285 are separated by 219 nucleotides BWI76_RS23265: BWI76_RS23265 - hypothetical protein, at 4,852,180 to 4,853,856 _RS23265 BWI76_RS23270: BWI76_RS23270 - phosphoenolpyruvate--protein phosphotransferase PtsP, at 4,854,084 to 4,856,330 _RS23270 BWI76_RS23275: BWI76_RS23275 - RNA pyrophosphohydrolase, at 4,856,343 to 4,856,873 _RS23275 BWI76_RS23280: BWI76_RS23280 - hypothetical protein, at 4,856,913 to 4,857,095 _RS23280 BWI76_RS23285: BWI76_RS23285 - hypothetical protein, at 4,857,315 to 4,857,539 _RS23285 Position (kb) 4854 4855 4856 4857Strain fitness (log2 ratio) -2 -1 0 1at 4854.079 kb on - strandat 4854.670 kb on + strand, within BWI76_RS23270at 4854.740 kb on + strand, within BWI76_RS23270at 4854.877 kb on + strand, within BWI76_RS23270at 4854.885 kb on - strand, within BWI76_RS23270at 4854.885 kb on - strand, within BWI76_RS23270at 4854.992 kb on + strand, within BWI76_RS23270at 4855.076 kb on - strand, within BWI76_RS23270at 4855.181 kb on + strand, within BWI76_RS23270at 4855.181 kb on + strand, within BWI76_RS23270at 4855.189 kb on - strand, within BWI76_RS23270at 4855.189 kb on - strand, within BWI76_RS23270at 4855.329 kb on + strand, within BWI76_RS23270at 4855.370 kb on + strand, within BWI76_RS23270at 4855.473 kb on - strand, within BWI76_RS23270at 4855.601 kb on + strand, within BWI76_RS23270at 4855.717 kb on + strand, within BWI76_RS23270at 4855.935 kb on - strand, within BWI76_RS23270at 4856.079 kb on - strand, within BWI76_RS23270at 4856.334 kb on - strandat 4856.389 kb on - strandat 4856.929 kb on + strandat 4856.995 kb on + strand, within BWI76_RS23280at 4857.274 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
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4,854,079 - -0.6
4,854,670 + BWI76_RS23270 0.26 -2.3
4,854,740 + BWI76_RS23270 0.29 -2.6
4,854,877 + BWI76_RS23270 0.35 -0.1
4,854,885 - BWI76_RS23270 0.36 -1.2
4,854,885 - BWI76_RS23270 0.36 -0.7
4,854,992 + BWI76_RS23270 0.40 -0.4
4,855,076 - BWI76_RS23270 0.44 -1.1
4,855,181 + BWI76_RS23270 0.49 -2.2
4,855,181 + BWI76_RS23270 0.49 +0.0
4,855,189 - BWI76_RS23270 0.49 -1.1
4,855,189 - BWI76_RS23270 0.49 -1.5
4,855,329 + BWI76_RS23270 0.55 -1.6
4,855,370 + BWI76_RS23270 0.57 -0.5
4,855,473 - BWI76_RS23270 0.62 -0.5
4,855,601 + BWI76_RS23270 0.68 -0.4
4,855,717 + BWI76_RS23270 0.73 -1.8
4,855,935 - BWI76_RS23270 0.82 -2.1
4,856,079 - BWI76_RS23270 0.89 -1.6
4,856,334 - +0.6
4,856,389 - +1.0
4,856,929 + -0.4
4,856,995 + BWI76_RS23280 0.45 -0.2
4,857,274 - +1.4

Or see this region's nucleotide sequence