Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16970

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS16960 and BWI76_RS16965 are separated by 311 nucleotidesBWI76_RS16965 and BWI76_RS16970 are separated by 64 nucleotidesBWI76_RS16970 and BWI76_RS16975 are separated by 101 nucleotidesBWI76_RS16975 and BWI76_RS16980 are separated by 11 nucleotides BWI76_RS16960: BWI76_RS16960 - pyruvate kinase, at 3,517,562 to 3,518,974 _RS16960 BWI76_RS16965: BWI76_RS16965 - murein lipoprotein, at 3,519,286 to 3,519,522 _RS16965 BWI76_RS16970: BWI76_RS16970 - murein L,D-transpeptidase, at 3,519,587 to 3,520,570 _RS16970 BWI76_RS16975: BWI76_RS16975 - cysteine desufuration protein SufE, at 3,520,672 to 3,521,088 _RS16975 BWI76_RS16980: BWI76_RS16980 - bifunctional cysteine desulfurase/selenocysteine lyase, at 3,521,100 to 3,522,320 _RS16980 Position (kb) 3519 3520 3521Strain fitness (log2 ratio) -2 -1 0 1at 3519.241 kb on + strandat 3519.249 kb on + strandat 3519.266 kb on - strandat 3519.268 kb on + strandat 3519.276 kb on - strandat 3519.293 kb on + strandat 3519.326 kb on + strand, within BWI76_RS16965at 3519.481 kb on + strand, within BWI76_RS16965at 3519.692 kb on - strand, within BWI76_RS16970at 3519.769 kb on - strand, within BWI76_RS16970at 3519.776 kb on - strand, within BWI76_RS16970at 3520.206 kb on + strand, within BWI76_RS16970at 3520.272 kb on - strand, within BWI76_RS16970at 3520.441 kb on - strand, within BWI76_RS16970at 3520.443 kb on + strand, within BWI76_RS16970at 3520.550 kb on + strandat 3520.648 kb on - strandat 3520.953 kb on + strand, within BWI76_RS16975at 3521.043 kb on + strand, within BWI76_RS16975at 3521.051 kb on - strandat 3521.158 kb on + strandat 3521.161 kb on + strandat 3521.169 kb on - strandat 3521.211 kb on - strandat 3521.367 kb on + strand, within BWI76_RS16980at 3521.390 kb on + strand, within BWI76_RS16980

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
remove
3,519,241 + +0.5
3,519,249 + +0.3
3,519,266 - +1.7
3,519,268 + +0.2
3,519,276 - +0.2
3,519,293 + +0.4
3,519,326 + BWI76_RS16965 0.17 +0.0
3,519,481 + BWI76_RS16965 0.82 -0.9
3,519,692 - BWI76_RS16970 0.11 +0.4
3,519,769 - BWI76_RS16970 0.18 +0.7
3,519,776 - BWI76_RS16970 0.19 +1.1
3,520,206 + BWI76_RS16970 0.63 +0.7
3,520,272 - BWI76_RS16970 0.70 +0.5
3,520,441 - BWI76_RS16970 0.87 +0.1
3,520,443 + BWI76_RS16970 0.87 +1.0
3,520,550 + +0.1
3,520,648 - +0.6
3,520,953 + BWI76_RS16975 0.67 -1.0
3,521,043 + BWI76_RS16975 0.89 -1.1
3,521,051 - -0.9
3,521,158 + -1.3
3,521,161 + -0.7
3,521,169 - -0.6
3,521,211 - -1.4
3,521,367 + BWI76_RS16980 0.22 -1.9
3,521,390 + BWI76_RS16980 0.24 -1.2

Or see this region's nucleotide sequence