Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01180

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01170 and BWI76_RS01175 are separated by 21 nucleotidesBWI76_RS01175 and BWI76_RS01180 overlap by 4 nucleotidesBWI76_RS01180 and BWI76_RS01185 are separated by 346 nucleotides BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995 _RS01170 BWI76_RS01175: BWI76_RS01175 - uroporphyrinogen-III synthase, at 240,017 to 240,757 _RS01175 BWI76_RS01180: BWI76_RS01180 - porphobilinogen deaminase, at 240,754 to 241,695 _RS01180 BWI76_RS01185: BWI76_RS01185 - adenylate cyclase, at 242,042 to 244,591 _RS01185 Position (kb) 240 241 242Strain fitness (log2 ratio) -2 -1 0 1at 239.939 kb on + strandat 241.724 kb on - strandat 241.765 kb on + strandat 241.857 kb on - strandat 241.938 kb on + strandat 242.047 kb on + strandat 242.055 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
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239,939 + +0.2
241,724 - -0.6
241,765 + +0.1
241,857 - +0.3
241,938 + -1.0
242,047 + -2.3
242,055 - -0.6

Or see this region's nucleotide sequence