Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01165

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01145 and BWI76_RS01150 are separated by 55 nucleotidesBWI76_RS01150 and BWI76_RS01155 are separated by 22 nucleotidesBWI76_RS01155 and BWI76_RS01160 are separated by 45 nucleotidesBWI76_RS01160 and BWI76_RS01165 are separated by 461 nucleotidesBWI76_RS01165 and BWI76_RS01170 are separated by 3 nucleotides BWI76_RS01145: BWI76_RS01145 - tRNA-Arg, at 236,726 to 236,802 _RS01145 BWI76_RS01150: BWI76_RS01150 - tRNA-His, at 236,858 to 236,933 _RS01150 BWI76_RS01155: BWI76_RS01155 - tRNA-Leu, at 236,956 to 237,042 _RS01155 BWI76_RS01160: BWI76_RS01160 - tRNA-Pro, at 237,088 to 237,164 _RS01160 BWI76_RS01165: BWI76_RS01165 - protoheme IX biogenesis protein HemY, at 237,626 to 238,822 _RS01165 BWI76_RS01170: BWI76_RS01170 - uroporphyrinogen-III C-methyltransferase, at 238,826 to 239,995 _RS01170 Position (kb) 237 238 239Strain fitness (log2 ratio) -2 -1 0 1at 237.382 kb on - strandat 237.411 kb on + strandat 237.651 kb on + strandat 237.742 kb on - strandat 237.830 kb on + strand, within BWI76_RS01165at 237.855 kb on + strand, within BWI76_RS01165at 237.889 kb on - strand, within BWI76_RS01165at 238.154 kb on + strand, within BWI76_RS01165at 238.162 kb on - strand, within BWI76_RS01165at 238.230 kb on + strand, within BWI76_RS01165at 238.564 kb on + strand, within BWI76_RS01165at 238.944 kb on - strand, within BWI76_RS01170at 239.119 kb on - strand, within BWI76_RS01170at 239.155 kb on + strand, within BWI76_RS01170at 239.528 kb on + strand, within BWI76_RS01170at 239.545 kb on + strand, within BWI76_RS01170

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
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237,382 - -0.8
237,411 + +0.5
237,651 + +0.0
237,742 - -0.1
237,830 + BWI76_RS01165 0.17 -0.0
237,855 + BWI76_RS01165 0.19 -2.1
237,889 - BWI76_RS01165 0.22 -0.3
238,154 + BWI76_RS01165 0.44 -1.4
238,162 - BWI76_RS01165 0.45 +0.8
238,230 + BWI76_RS01165 0.50 -0.4
238,564 + BWI76_RS01165 0.78 +0.1
238,944 - BWI76_RS01170 0.10 -0.0
239,119 - BWI76_RS01170 0.25 +0.6
239,155 + BWI76_RS01170 0.28 -0.1
239,528 + BWI76_RS01170 0.60 +1.2
239,545 + BWI76_RS01170 0.61 -0.2

Or see this region's nucleotide sequence