Experiment: Plant=Brachypodium_distachyon; PreTreatment=None; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=R2A_with_kan10; Time=21days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_000217 and ABIE53_000218 are separated by 174 nucleotides ABIE53_000218 and ABIE53_000219 are separated by 71 nucleotides ABIE53_000219 and ABIE53_000220 are separated by 58 nucleotides ABIE53_000220 and ABIE53_000221 overlap by 17 nucleotides ABIE53_000221 and ABIE53_000222 overlap by 8 nucleotides
ABIE53_000217: ABIE53_000217 - hypothetical protein, at 224,012 to 224,290
_000217
ABIE53_000218: ABIE53_000218 - DNA-binding transcriptional MerR regulator, at 224,465 to 224,677
_000218
ABIE53_000219: ABIE53_000219 - excisionase family DNA binding protein, at 224,749 to 224,949
_000219
ABIE53_000220: ABIE53_000220 - hypothetical protein, at 225,008 to 225,331
_000220
ABIE53_000221: ABIE53_000221 - hypothetical protein, at 225,315 to 226,325
_000221
ABIE53_000222: ABIE53_000222 - hypothetical protein, at 226,318 to 226,605
_000222
Position (kb) 225
226 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 224.013 kb on + strand at 224.013 kb on + strand at 224.013 kb on + strand at 224.014 kb on - strand at 224.014 kb on - strand at 224.033 kb on - strand at 224.033 kb on - strand at 224.097 kb on - strand, within ABIE53_000217 at 224.205 kb on + strand, within ABIE53_000217 at 224.205 kb on + strand, within ABIE53_000217 at 224.205 kb on + strand, within ABIE53_000217 at 224.205 kb on + strand, within ABIE53_000217 at 224.205 kb on + strand, within ABIE53_000217 at 224.205 kb on + strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.206 kb on - strand, within ABIE53_000217 at 224.228 kb on + strand, within ABIE53_000217 at 224.229 kb on - strand, within ABIE53_000217 at 224.229 kb on - strand, within ABIE53_000217 at 224.229 kb on - strand, within ABIE53_000217 at 224.229 kb on - strand, within ABIE53_000217 at 224.229 kb on - strand, within ABIE53_000217 at 224.284 kb on - strand at 224.290 kb on + strand at 224.290 kb on + strand at 224.338 kb on + strand at 224.338 kb on + strand at 224.338 kb on + strand at 224.339 kb on - strand at 224.340 kb on + strand at 224.341 kb on - strand at 224.399 kb on + strand at 224.400 kb on - strand at 224.400 kb on - strand at 224.400 kb on - strand at 224.410 kb on + strand at 224.411 kb on - strand at 224.411 kb on - strand at 224.411 kb on - strand at 224.455 kb on + strand at 224.456 kb on - strand at 224.456 kb on - strand at 224.508 kb on + strand, within ABIE53_000218 at 224.508 kb on + strand, within ABIE53_000218 at 224.508 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.571 kb on + strand, within ABIE53_000218 at 224.572 kb on - strand, within ABIE53_000218 at 224.572 kb on - strand, within ABIE53_000218 at 224.572 kb on - strand, within ABIE53_000218 at 224.572 kb on - strand, within ABIE53_000218 at 224.572 kb on - strand, within ABIE53_000218 at 224.626 kb on + strand, within ABIE53_000218 at 224.648 kb on + strand, within ABIE53_000218 at 224.648 kb on + strand, within ABIE53_000218 at 224.649 kb on - strand, within ABIE53_000218 at 224.649 kb on - strand, within ABIE53_000218 at 224.649 kb on - strand, within ABIE53_000218 at 224.649 kb on - strand, within ABIE53_000218 at 224.649 kb on - strand, within ABIE53_000218 at 224.649 kb on - strand, within ABIE53_000218 at 224.677 kb on + strand at 224.677 kb on + strand at 224.678 kb on - strand at 224.678 kb on - strand at 224.678 kb on - strand at 224.678 kb on - strand at 224.678 kb on - strand at 224.678 kb on - strand at 224.678 kb on - strand at 224.681 kb on + strand at 224.681 kb on + strand at 224.681 kb on + strand at 224.681 kb on + strand at 224.681 kb on + strand at 224.681 kb on + strand at 224.750 kb on + strand at 224.750 kb on + strand at 224.751 kb on - strand at 224.751 kb on - strand at 224.813 kb on + strand, within ABIE53_000219 at 224.847 kb on + strand, within ABIE53_000219 at 224.847 kb on + strand, within ABIE53_000219 at 224.847 kb on + strand, within ABIE53_000219 at 224.847 kb on + strand, within ABIE53_000219 at 224.847 kb on + strand, within ABIE53_000219 at 224.890 kb on + strand, within ABIE53_000219 at 224.891 kb on - strand, within ABIE53_000219 at 224.891 kb on - strand, within ABIE53_000219 at 224.891 kb on - strand, within ABIE53_000219 at 224.903 kb on + strand, within ABIE53_000219 at 224.903 kb on + strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.904 kb on - strand, within ABIE53_000219 at 224.950 kb on - strand at 224.950 kb on - strand at 224.950 kb on - strand at 224.950 kb on - strand at 224.967 kb on + strand at 224.967 kb on + strand at 224.967 kb on + strand at 224.967 kb on + strand at 224.968 kb on - strand at 224.986 kb on + strand at 224.986 kb on + strand at 224.986 kb on + strand at 224.986 kb on + strand at 224.986 kb on + strand at 224.987 kb on - strand at 225.010 kb on - strand at 225.132 kb on + strand, within ABIE53_000220 at 225.133 kb on - strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.188 kb on + strand, within ABIE53_000220 at 225.189 kb on - strand, within ABIE53_000220 at 225.189 kb on - strand, within ABIE53_000220 at 225.189 kb on - strand, within ABIE53_000220 at 225.189 kb on - strand, within ABIE53_000220 at 225.189 kb on - strand, within ABIE53_000220 at 225.265 kb on + strand, within ABIE53_000220 at 225.266 kb on - strand, within ABIE53_000220 at 225.266 kb on - strand, within ABIE53_000220 at 225.266 kb on - strand, within ABIE53_000220 at 225.282 kb on - strand, within ABIE53_000220 at 225.362 kb on + strand at 225.362 kb on + strand at 225.362 kb on + strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.369 kb on - strand at 225.408 kb on - strand at 225.531 kb on - strand, within ABIE53_000221 at 225.572 kb on - strand, within ABIE53_000221 at 225.572 kb on - strand, within ABIE53_000221 at 225.579 kb on + strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.580 kb on - strand, within ABIE53_000221 at 225.647 kb on - strand, within ABIE53_000221 at 225.691 kb on + strand, within ABIE53_000221 at 225.691 kb on + strand, within ABIE53_000221 at 225.692 kb on - strand, within ABIE53_000221 at 225.692 kb on - strand, within ABIE53_000221 at 225.692 kb on - strand, within ABIE53_000221 at 225.747 kb on + strand, within ABIE53_000221 at 225.858 kb on + strand, within ABIE53_000221 at 225.858 kb on + strand, within ABIE53_000221 at 225.859 kb on - strand, within ABIE53_000221 at 225.859 kb on - strand, within ABIE53_000221 at 225.859 kb on - strand, within ABIE53_000221 at 225.925 kb on + strand, within ABIE53_000221 at 225.925 kb on + strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.926 kb on - strand, within ABIE53_000221 at 225.970 kb on - strand, within ABIE53_000221 at 226.029 kb on + strand, within ABIE53_000221 at 226.080 kb on + strand, within ABIE53_000221 at 226.080 kb on + strand, within ABIE53_000221 at 226.080 kb on + strand, within ABIE53_000221 at 226.081 kb on - strand, within ABIE53_000221 at 226.081 kb on - strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.132 kb on + strand, within ABIE53_000221 at 226.133 kb on - strand, within ABIE53_000221 at 226.133 kb on - strand, within ABIE53_000221 at 226.143 kb on + strand, within ABIE53_000221 at 226.144 kb on - strand, within ABIE53_000221 at 226.257 kb on + strand at 226.257 kb on + strand at 226.258 kb on - strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.277 kb on - strand at 226.277 kb on - strand at 226.281 kb on - strand at 226.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Plant=Brachypodium_distachyon; PreTreatment=None; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=R2A_with_kan10; Time=21days remove 224,013 + +0.2 224,013 + -1.2 224,013 + +2.2 224,014 - -2.1 224,014 - +0.5 224,033 - +2.8 224,033 - -2.1 224,097 - ABIE53_000217 0.30 -1.4 224,205 + ABIE53_000217 0.69 -1.8 224,205 + ABIE53_000217 0.69 -0.7 224,205 + ABIE53_000217 0.69 +8.3 224,205 + ABIE53_000217 0.69 -2.6 224,205 + ABIE53_000217 0.69 -0.7 224,205 + ABIE53_000217 0.69 -1.0 224,206 - ABIE53_000217 0.70 -2.5 224,206 - ABIE53_000217 0.70 +2.5 224,206 - ABIE53_000217 0.70 +0.0 224,206 - ABIE53_000217 0.70 -2.8 224,206 - ABIE53_000217 0.70 +2.5 224,206 - ABIE53_000217 0.70 +1.7 224,206 - ABIE53_000217 0.70 -0.5 224,206 - ABIE53_000217 0.70 +0.9 224,206 - ABIE53_000217 0.70 -0.6 224,206 - ABIE53_000217 0.70 -1.1 224,228 + ABIE53_000217 0.77 -0.3 224,229 - ABIE53_000217 0.78 +0.9 224,229 - ABIE53_000217 0.78 +1.3 224,229 - ABIE53_000217 0.78 -0.8 224,229 - ABIE53_000217 0.78 -0.7 224,229 - ABIE53_000217 0.78 +2.5 224,284 - -0.8 224,290 + -0.1 224,290 + +2.5 224,338 + +0.5 224,338 + +0.2 224,338 + -0.1 224,339 - -2.6 224,340 + -2.3 224,341 - -0.1 224,399 + -3.7 224,400 - +0.2 224,400 - -0.1 224,400 - -1.8 224,410 + -2.7 224,411 - +4.4 224,411 - +0.6 224,411 - -0.9 224,455 + -0.1 224,456 - +1.6 224,456 - -0.8 224,508 + ABIE53_000218 0.20 +0.5 224,508 + ABIE53_000218 0.20 -0.1 224,508 + ABIE53_000218 0.20 -1.2 224,571 + ABIE53_000218 0.50 -2.4 224,571 + ABIE53_000218 0.50 -1.1 224,571 + ABIE53_000218 0.50 -2.3 224,571 + ABIE53_000218 0.50 +0.7 224,571 + ABIE53_000218 0.50 +1.2 224,571 + ABIE53_000218 0.50 -0.3 224,571 + ABIE53_000218 0.50 +1.0 224,572 - ABIE53_000218 0.50 +0.5 224,572 - ABIE53_000218 0.50 -0.5 224,572 - ABIE53_000218 0.50 -0.8 224,572 - ABIE53_000218 0.50 -1.0 224,572 - ABIE53_000218 0.50 -0.1 224,626 + ABIE53_000218 0.76 -0.0 224,648 + ABIE53_000218 0.86 +0.4 224,648 + ABIE53_000218 0.86 -1.3 224,649 - ABIE53_000218 0.86 -1.8 224,649 - ABIE53_000218 0.86 -2.0 224,649 - ABIE53_000218 0.86 -0.3 224,649 - ABIE53_000218 0.86 -1.1 224,649 - ABIE53_000218 0.86 +3.0 224,649 - ABIE53_000218 0.86 -0.5 224,677 + -0.1 224,677 + +2.1 224,678 - +0.5 224,678 - +0.7 224,678 - +0.5 224,678 - +0.5 224,678 - +1.6 224,678 - -1.6 224,678 - -2.8 224,681 + +0.5 224,681 + +2.5 224,681 + -0.7 224,681 + -2.7 224,681 + +0.5 224,681 + -0.5 224,750 + -1.5 224,750 + -0.2 224,751 - -0.5 224,751 - +0.5 224,813 + ABIE53_000219 0.32 -0.1 224,847 + ABIE53_000219 0.49 -1.9 224,847 + ABIE53_000219 0.49 +1.1 224,847 + ABIE53_000219 0.49 -0.3 224,847 + ABIE53_000219 0.49 -2.2 224,847 + ABIE53_000219 0.49 -0.3 224,890 + ABIE53_000219 0.70 -0.7 224,891 - ABIE53_000219 0.71 +1.4 224,891 - ABIE53_000219 0.71 +1.2 224,891 - ABIE53_000219 0.71 -0.5 224,903 + ABIE53_000219 0.77 -0.3 224,903 + ABIE53_000219 0.77 +0.2 224,904 - ABIE53_000219 0.77 -1.0 224,904 - ABIE53_000219 0.77 -0.5 224,904 - ABIE53_000219 0.77 -1.1 224,904 - ABIE53_000219 0.77 -1.0 224,904 - ABIE53_000219 0.77 -1.7 224,904 - ABIE53_000219 0.77 +0.0 224,904 - ABIE53_000219 0.77 -2.0 224,904 - ABIE53_000219 0.77 +0.6 224,904 - ABIE53_000219 0.77 +0.2 224,904 - ABIE53_000219 0.77 -1.0 224,904 - ABIE53_000219 0.77 -0.8 224,904 - ABIE53_000219 0.77 +1.7 224,904 - ABIE53_000219 0.77 +0.8 224,950 - -0.1 224,950 - -1.8 224,950 - +2.5 224,950 - -0.5 224,967 + -1.5 224,967 + -2.8 224,967 + -0.3 224,967 + +0.6 224,968 - +0.0 224,986 + +3.0 224,986 + -0.7 224,986 + +0.2 224,986 + +0.2 224,986 + -0.8 224,987 - +2.5 225,010 - -0.9 225,132 + ABIE53_000220 0.38 +1.1 225,133 - ABIE53_000220 0.39 -0.1 225,188 + ABIE53_000220 0.56 +1.5 225,188 + ABIE53_000220 0.56 +0.2 225,188 + ABIE53_000220 0.56 -1.6 225,188 + ABIE53_000220 0.56 -0.3 225,188 + ABIE53_000220 0.56 -0.7 225,188 + ABIE53_000220 0.56 -0.5 225,188 + ABIE53_000220 0.56 -2.7 225,188 + ABIE53_000220 0.56 -1.4 225,189 - ABIE53_000220 0.56 -2.1 225,189 - ABIE53_000220 0.56 -0.8 225,189 - ABIE53_000220 0.56 -0.3 225,189 - ABIE53_000220 0.56 -1.1 225,189 - ABIE53_000220 0.56 +0.5 225,265 + ABIE53_000220 0.79 +0.5 225,266 - ABIE53_000220 0.80 +0.5 225,266 - ABIE53_000220 0.80 -0.4 225,266 - ABIE53_000220 0.80 +1.8 225,282 - ABIE53_000220 0.85 -2.8 225,362 + -2.6 225,362 + +0.1 225,362 + -0.5 225,369 - -0.1 225,369 - -0.7 225,369 - -0.0 225,369 - +0.5 225,369 - +1.0 225,369 - -1.8 225,408 - -0.7 225,531 - ABIE53_000221 0.21 +1.1 225,572 - ABIE53_000221 0.25 -1.8 225,572 - ABIE53_000221 0.25 +1.4 225,579 + ABIE53_000221 0.26 -0.7 225,580 - ABIE53_000221 0.26 -2.8 225,580 - ABIE53_000221 0.26 +1.5 225,580 - ABIE53_000221 0.26 -2.8 225,580 - ABIE53_000221 0.26 -2.3 225,647 - ABIE53_000221 0.33 -2.2 225,691 + ABIE53_000221 0.37 -1.5 225,691 + ABIE53_000221 0.37 -0.3 225,692 - ABIE53_000221 0.37 -1.7 225,692 - ABIE53_000221 0.37 -0.1 225,692 - ABIE53_000221 0.37 -0.5 225,747 + ABIE53_000221 0.43 +3.2 225,858 + ABIE53_000221 0.54 -0.8 225,858 + ABIE53_000221 0.54 +0.9 225,859 - ABIE53_000221 0.54 -0.1 225,859 - ABIE53_000221 0.54 +0.9 225,859 - ABIE53_000221 0.54 +0.9 225,925 + ABIE53_000221 0.60 -0.6 225,925 + ABIE53_000221 0.60 +0.8 225,926 - ABIE53_000221 0.60 -1.3 225,926 - ABIE53_000221 0.60 -0.3 225,926 - ABIE53_000221 0.60 -0.1 225,926 - ABIE53_000221 0.60 -1.0 225,926 - ABIE53_000221 0.60 -2.4 225,970 - ABIE53_000221 0.65 +0.6 226,029 + ABIE53_000221 0.71 -1.6 226,080 + ABIE53_000221 0.76 -1.2 226,080 + ABIE53_000221 0.76 -1.4 226,080 + ABIE53_000221 0.76 +1.2 226,081 - ABIE53_000221 0.76 +0.0 226,081 - ABIE53_000221 0.76 +1.5 226,132 + ABIE53_000221 0.81 -1.8 226,132 + ABIE53_000221 0.81 +0.1 226,132 + ABIE53_000221 0.81 -0.1 226,132 + ABIE53_000221 0.81 +2.7 226,132 + ABIE53_000221 0.81 +1.5 226,133 - ABIE53_000221 0.81 -0.3 226,133 - ABIE53_000221 0.81 -1.2 226,143 + ABIE53_000221 0.82 +1.2 226,144 - ABIE53_000221 0.82 -1.1 226,257 + +1.5 226,257 + -0.2 226,258 - -0.5 226,276 + -1.0 226,276 + +0.9 226,276 + +0.8 226,276 + -0.5 226,277 - -1.8 226,277 - -0.5 226,281 - +0.6 226,320 - -2.4
Or see this region's nucleotide sequence