Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_498 and Pf6N2E2_499 are separated by 608 nucleotides Pf6N2E2_499 and Pf6N2E2_500 are separated by 78 nucleotides
Pf6N2E2_498 - Dihydropyrimidinase (EC 3.5.2.2) <small>(from data)</small>, at 565,573 to 567,012
_498
Pf6N2E2_499 - Possible pyrimidine permease in reductive pathway, at 567,621 to 569,099
_499
Pf6N2E2_500 - Beta-ureidopropionase (EC 3.5.1.6), at 569,178 to 570,461
_500
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.865 kb on - strand, within Pf6N2E2_498 at 566.979 kb on + strand at 567.061 kb on + strand at 567.062 kb on - strand at 567.077 kb on + strand at 567.095 kb on + strand at 567.096 kb on - strand at 567.181 kb on - strand at 567.288 kb on + strand at 567.392 kb on - strand at 567.406 kb on + strand at 567.473 kb on + strand at 567.473 kb on + strand at 567.475 kb on + strand at 567.567 kb on + strand at 567.567 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.702 kb on - strand at 567.798 kb on + strand, within Pf6N2E2_499 at 567.798 kb on + strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 568.317 kb on + strand, within Pf6N2E2_499 at 568.317 kb on + strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.318 kb on - strand, within Pf6N2E2_499 at 568.783 kb on - strand, within Pf6N2E2_499 at 568.848 kb on + strand, within Pf6N2E2_499 at 568.849 kb on - strand, within Pf6N2E2_499 at 568.905 kb on + strand, within Pf6N2E2_499 at 568.998 kb on + strand at 568.998 kb on + strand at 568.999 kb on - strand at 569.043 kb on + strand at 569.200 kb on + strand at 569.200 kb on + strand at 569.201 kb on - strand at 569.760 kb on + strand, within Pf6N2E2_500 at 569.761 kb on - strand, within Pf6N2E2_500 at 569.909 kb on + strand, within Pf6N2E2_500 at 569.910 kb on - strand, within Pf6N2E2_500
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 566,829 + Pf6N2E2_498 0.87 -1.5 566,829 + Pf6N2E2_498 0.87 -2.5 566,829 + Pf6N2E2_498 0.87 -2.4 566,830 - Pf6N2E2_498 0.87 -2.0 566,830 - Pf6N2E2_498 0.87 -1.6 566,864 + Pf6N2E2_498 0.90 -1.3 566,864 + Pf6N2E2_498 0.90 -1.1 566,864 + Pf6N2E2_498 0.90 -0.3 566,865 - Pf6N2E2_498 0.90 -2.3 566,979 + -1.9 567,061 + -1.4 567,062 - -0.6 567,077 + -2.0 567,095 + -0.8 567,096 - -0.9 567,181 - +0.9 567,288 + -0.9 567,392 - -1.8 567,406 + -1.8 567,473 + -0.2 567,473 + -1.2 567,475 + -1.1 567,567 + -1.0 567,567 + -1.9 567,701 + -1.9 567,701 + -3.0 567,701 + -1.3 567,702 - -2.1 567,798 + Pf6N2E2_499 0.12 -2.1 567,798 + Pf6N2E2_499 0.12 +1.2 567,799 - Pf6N2E2_499 0.12 -0.9 567,799 - Pf6N2E2_499 0.12 -2.1 568,317 + Pf6N2E2_499 0.47 -1.7 568,317 + Pf6N2E2_499 0.47 -3.3 568,318 - Pf6N2E2_499 0.47 -2.8 568,318 - Pf6N2E2_499 0.47 -1.3 568,318 - Pf6N2E2_499 0.47 -2.8 568,783 - Pf6N2E2_499 0.79 -2.5 568,848 + Pf6N2E2_499 0.83 -0.4 568,849 - Pf6N2E2_499 0.83 -1.1 568,905 + Pf6N2E2_499 0.87 +0.2 568,998 + -0.1 568,998 + -0.3 568,999 - -1.8 569,043 + +0.2 569,200 + -1.1 569,200 + -0.9 569,201 - -1.0 569,760 + Pf6N2E2_500 0.45 -1.6 569,761 - Pf6N2E2_500 0.45 -2.9 569,909 + Pf6N2E2_500 0.57 -0.8 569,910 - Pf6N2E2_500 0.57 -1.2
Or see this region's nucleotide sequence