Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_34 and Pf6N2E2_35 are separated by 12 nucleotides Pf6N2E2_35 and Pf6N2E2_36 are separated by 186 nucleotides
Pf6N2E2_34 - Cytosine deaminase (EC 3.5.4.1), at 73,056 to 74,303
_34
Pf6N2E2_35 - Cytosine permease, at 74,316 to 75,587
_35
Pf6N2E2_36 - Possible oxidoreductase, at 75,774 to 76,604
_36
Position (kb)
74
75
76 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 73.340 kb on + strand, within Pf6N2E2_34 at 73.458 kb on - strand, within Pf6N2E2_34 at 73.549 kb on + strand, within Pf6N2E2_34 at 73.550 kb on - strand, within Pf6N2E2_34 at 73.561 kb on + strand, within Pf6N2E2_34 at 73.562 kb on - strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 74.317 kb on + strand at 74.317 kb on + strand at 74.359 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.593 kb on + strand, within Pf6N2E2_35 at 74.594 kb on - strand, within Pf6N2E2_35 at 74.612 kb on - strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.822 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.139 kb on + strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.253 kb on + strand, within Pf6N2E2_35 at 75.362 kb on + strand, within Pf6N2E2_35 at 75.365 kb on - strand, within Pf6N2E2_35 at 75.365 kb on - strand, within Pf6N2E2_35 at 75.382 kb on + strand, within Pf6N2E2_35 at 75.382 kb on + strand, within Pf6N2E2_35 at 75.560 kb on - strand at 75.560 kb on - strand at 75.560 kb on - strand at 75.612 kb on + strand at 75.612 kb on + strand at 75.612 kb on + strand at 75.612 kb on + strand at 75.612 kb on + strand at 75.612 kb on + strand at 75.612 kb on + strand at 75.613 kb on - strand at 75.613 kb on - strand at 75.613 kb on - strand at 75.613 kb on - strand at 75.659 kb on + strand at 75.660 kb on - strand at 75.660 kb on - strand at 75.660 kb on - strand at 75.660 kb on - strand at 75.663 kb on + strand at 75.663 kb on + strand at 76.003 kb on + strand, within Pf6N2E2_36 at 76.003 kb on + strand, within Pf6N2E2_36 at 76.003 kb on + strand, within Pf6N2E2_36 at 76.075 kb on + strand, within Pf6N2E2_36 at 76.076 kb on - strand, within Pf6N2E2_36 at 76.076 kb on - strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.126 kb on + strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.127 kb on - strand, within Pf6N2E2_36 at 76.153 kb on + strand, within Pf6N2E2_36 at 76.153 kb on + strand, within Pf6N2E2_36 at 76.153 kb on + strand, within Pf6N2E2_36 at 76.154 kb on - strand, within Pf6N2E2_36 at 76.154 kb on - strand, within Pf6N2E2_36 at 76.154 kb on - strand, within Pf6N2E2_36 at 76.272 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.438 kb on + strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.439 kb on - strand, within Pf6N2E2_36 at 76.489 kb on + strand, within Pf6N2E2_36 at 76.489 kb on + strand, within Pf6N2E2_36 at 76.489 kb on + strand, within Pf6N2E2_36 at 76.490 kb on - strand, within Pf6N2E2_36 at 76.490 kb on - strand, within Pf6N2E2_36 at 76.576 kb on + strand at 76.577 kb on - strand at 76.577 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 73,340 + Pf6N2E2_34 0.23 -1.9 73,458 - Pf6N2E2_34 0.32 -2.1 73,549 + Pf6N2E2_34 0.40 -1.5 73,550 - Pf6N2E2_34 0.40 -2.3 73,561 + Pf6N2E2_34 0.40 -3.1 73,562 - Pf6N2E2_34 0.41 -1.1 73,756 + Pf6N2E2_34 0.56 -0.1 73,756 + Pf6N2E2_34 0.56 -1.9 74,317 + -1.4 74,317 + -2.7 74,359 - -0.5 74,371 - +0.4 74,371 - +0.0 74,371 - +0.2 74,371 - +1.0 74,371 - +0.0 74,377 + +0.1 74,377 + -2.4 74,377 + +0.6 74,377 + -1.0 74,377 + -2.6 74,377 + -0.5 74,378 - +0.3 74,378 - -0.9 74,378 - -0.4 74,378 - -3.4 74,593 + Pf6N2E2_35 0.22 -0.7 74,594 - Pf6N2E2_35 0.22 -0.2 74,612 - Pf6N2E2_35 0.23 -0.2 74,716 + Pf6N2E2_35 0.31 -2.4 74,716 + Pf6N2E2_35 0.31 -0.8 74,716 + Pf6N2E2_35 0.31 -0.0 74,716 + Pf6N2E2_35 0.31 -2.9 74,716 + Pf6N2E2_35 0.31 -0.8 74,717 - Pf6N2E2_35 0.32 -0.9 74,717 - Pf6N2E2_35 0.32 -0.7 74,821 + Pf6N2E2_35 0.40 -0.7 74,821 + Pf6N2E2_35 0.40 -0.5 74,821 + Pf6N2E2_35 0.40 -0.9 74,821 + Pf6N2E2_35 0.40 -0.5 74,821 + Pf6N2E2_35 0.40 -2.1 74,822 - Pf6N2E2_35 0.40 -1.9 75,107 - Pf6N2E2_35 0.62 -1.4 75,107 - Pf6N2E2_35 0.62 -0.3 75,139 + Pf6N2E2_35 0.65 -1.1 75,140 - Pf6N2E2_35 0.65 -0.8 75,140 - Pf6N2E2_35 0.65 -0.1 75,211 + Pf6N2E2_35 0.70 -0.4 75,211 + Pf6N2E2_35 0.70 -1.1 75,211 + Pf6N2E2_35 0.70 -1.4 75,212 - Pf6N2E2_35 0.70 -0.4 75,212 - Pf6N2E2_35 0.70 -1.2 75,253 + Pf6N2E2_35 0.74 +0.1 75,362 + Pf6N2E2_35 0.82 -0.4 75,365 - Pf6N2E2_35 0.82 +0.7 75,365 - Pf6N2E2_35 0.82 +0.1 75,382 + Pf6N2E2_35 0.84 +0.7 75,382 + Pf6N2E2_35 0.84 -2.6 75,560 - -1.5 75,560 - -1.2 75,560 - -0.3 75,612 + -1.6 75,612 + -0.9 75,612 + +0.1 75,612 + -2.0 75,612 + -1.0 75,612 + -1.4 75,612 + +1.1 75,613 - +0.5 75,613 - -0.5 75,613 - +0.2 75,613 - +0.2 75,659 + -0.8 75,660 - +0.9 75,660 - -2.3 75,660 - -0.2 75,660 - +1.1 75,663 + +0.1 75,663 + -0.5 76,003 + Pf6N2E2_36 0.28 +1.2 76,003 + Pf6N2E2_36 0.28 +0.3 76,003 + Pf6N2E2_36 0.28 +0.6 76,075 + Pf6N2E2_36 0.36 +0.7 76,076 - Pf6N2E2_36 0.36 +0.5 76,076 - Pf6N2E2_36 0.36 +0.9 76,126 + Pf6N2E2_36 0.42 +0.1 76,126 + Pf6N2E2_36 0.42 -0.1 76,126 + Pf6N2E2_36 0.42 +0.1 76,126 + Pf6N2E2_36 0.42 +0.4 76,126 + Pf6N2E2_36 0.42 -1.1 76,126 + Pf6N2E2_36 0.42 +0.5 76,126 + Pf6N2E2_36 0.42 +0.1 76,127 - Pf6N2E2_36 0.42 +0.9 76,127 - Pf6N2E2_36 0.42 -0.6 76,127 - Pf6N2E2_36 0.42 +0.9 76,127 - Pf6N2E2_36 0.42 -0.4 76,127 - Pf6N2E2_36 0.42 -0.7 76,127 - Pf6N2E2_36 0.42 +0.4 76,127 - Pf6N2E2_36 0.42 +0.0 76,127 - Pf6N2E2_36 0.42 +1.5 76,153 + Pf6N2E2_36 0.46 -1.0 76,153 + Pf6N2E2_36 0.46 +0.0 76,153 + Pf6N2E2_36 0.46 -0.6 76,154 - Pf6N2E2_36 0.46 -0.1 76,154 - Pf6N2E2_36 0.46 +0.6 76,154 - Pf6N2E2_36 0.46 +0.9 76,272 + Pf6N2E2_36 0.60 -0.9 76,438 + Pf6N2E2_36 0.80 -0.2 76,438 + Pf6N2E2_36 0.80 -0.3 76,438 + Pf6N2E2_36 0.80 -0.7 76,438 + Pf6N2E2_36 0.80 -1.2 76,438 + Pf6N2E2_36 0.80 +0.0 76,438 + Pf6N2E2_36 0.80 -0.4 76,438 + Pf6N2E2_36 0.80 +0.2 76,438 + Pf6N2E2_36 0.80 +1.3 76,439 - Pf6N2E2_36 0.80 -0.5 76,439 - Pf6N2E2_36 0.80 -3.0 76,439 - Pf6N2E2_36 0.80 +0.9 76,439 - Pf6N2E2_36 0.80 +0.6 76,439 - Pf6N2E2_36 0.80 +0.3 76,439 - Pf6N2E2_36 0.80 +0.4 76,439 - Pf6N2E2_36 0.80 +0.5 76,439 - Pf6N2E2_36 0.80 -0.2 76,489 + Pf6N2E2_36 0.86 -0.5 76,489 + Pf6N2E2_36 0.86 -0.0 76,489 + Pf6N2E2_36 0.86 -1.6 76,490 - Pf6N2E2_36 0.86 -1.1 76,490 - Pf6N2E2_36 0.86 +0.4 76,576 + +0.4 76,577 - -1.6 76,577 - -0.7
Or see this region's nucleotide sequence