Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_33 and Pf6N2E2_34 are separated by 56 nucleotides Pf6N2E2_34 and Pf6N2E2_35 are separated by 12 nucleotides
Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999
_33
Pf6N2E2_34 - Cytosine deaminase (EC 3.5.4.1), at 73,056 to 74,303
_34
Pf6N2E2_35 - Cytosine permease, at 74,316 to 75,587
_35
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 72.255 kb on + strand, within Pf6N2E2_33 at 72.694 kb on + strand, within Pf6N2E2_33 at 72.695 kb on - strand, within Pf6N2E2_33 at 73.130 kb on - strand at 73.162 kb on + strand at 73.163 kb on - strand at 73.340 kb on + strand, within Pf6N2E2_34 at 73.458 kb on - strand, within Pf6N2E2_34 at 73.549 kb on + strand, within Pf6N2E2_34 at 73.550 kb on - strand, within Pf6N2E2_34 at 73.561 kb on + strand, within Pf6N2E2_34 at 73.562 kb on - strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 73.756 kb on + strand, within Pf6N2E2_34 at 74.317 kb on + strand at 74.317 kb on + strand at 74.359 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.371 kb on - strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.377 kb on + strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.378 kb on - strand at 74.593 kb on + strand, within Pf6N2E2_35 at 74.594 kb on - strand, within Pf6N2E2_35 at 74.612 kb on - strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.716 kb on + strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.717 kb on - strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.821 kb on + strand, within Pf6N2E2_35 at 74.822 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.107 kb on - strand, within Pf6N2E2_35 at 75.139 kb on + strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.140 kb on - strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.211 kb on + strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.212 kb on - strand, within Pf6N2E2_35 at 75.253 kb on + strand, within Pf6N2E2_35
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 72,255 + Pf6N2E2_33 0.26 -0.1 72,694 + Pf6N2E2_33 0.69 +0.2 72,695 - Pf6N2E2_33 0.70 +2.5 73,130 - -1.0 73,162 + -1.9 73,163 - -1.3 73,340 + Pf6N2E2_34 0.23 -1.9 73,458 - Pf6N2E2_34 0.32 -2.1 73,549 + Pf6N2E2_34 0.40 -1.5 73,550 - Pf6N2E2_34 0.40 -2.3 73,561 + Pf6N2E2_34 0.40 -3.1 73,562 - Pf6N2E2_34 0.41 -1.1 73,756 + Pf6N2E2_34 0.56 -0.1 73,756 + Pf6N2E2_34 0.56 -1.9 74,317 + -1.4 74,317 + -2.7 74,359 - -0.5 74,371 - +0.4 74,371 - +0.0 74,371 - +0.2 74,371 - +1.0 74,371 - +0.0 74,377 + +0.1 74,377 + -2.4 74,377 + +0.6 74,377 + -1.0 74,377 + -2.6 74,377 + -0.5 74,378 - +0.3 74,378 - -0.9 74,378 - -0.4 74,378 - -3.4 74,593 + Pf6N2E2_35 0.22 -0.7 74,594 - Pf6N2E2_35 0.22 -0.2 74,612 - Pf6N2E2_35 0.23 -0.2 74,716 + Pf6N2E2_35 0.31 -2.4 74,716 + Pf6N2E2_35 0.31 -0.8 74,716 + Pf6N2E2_35 0.31 -0.0 74,716 + Pf6N2E2_35 0.31 -2.9 74,716 + Pf6N2E2_35 0.31 -0.8 74,717 - Pf6N2E2_35 0.32 -0.9 74,717 - Pf6N2E2_35 0.32 -0.7 74,821 + Pf6N2E2_35 0.40 -0.7 74,821 + Pf6N2E2_35 0.40 -0.5 74,821 + Pf6N2E2_35 0.40 -0.9 74,821 + Pf6N2E2_35 0.40 -0.5 74,821 + Pf6N2E2_35 0.40 -2.1 74,822 - Pf6N2E2_35 0.40 -1.9 75,107 - Pf6N2E2_35 0.62 -1.4 75,107 - Pf6N2E2_35 0.62 -0.3 75,139 + Pf6N2E2_35 0.65 -1.1 75,140 - Pf6N2E2_35 0.65 -0.8 75,140 - Pf6N2E2_35 0.65 -0.1 75,211 + Pf6N2E2_35 0.70 -0.4 75,211 + Pf6N2E2_35 0.70 -1.1 75,211 + Pf6N2E2_35 0.70 -1.4 75,212 - Pf6N2E2_35 0.70 -0.4 75,212 - Pf6N2E2_35 0.70 -1.2 75,253 + Pf6N2E2_35 0.74 +0.1
Or see this region's nucleotide sequence