Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2602 and Pf6N2E2_2603 are separated by 249 nucleotides Pf6N2E2_2603 and Pf6N2E2_2604 are separated by 83 nucleotides Pf6N2E2_2604 and Pf6N2E2_2605 are separated by 83 nucleotides Pf6N2E2_2605 and Pf6N2E2_2606 overlap by 18 nucleotides
Pf6N2E2_2602 - Flagellin protein FlaA, at 3,059,480 to 3,060,331
_2602
Pf6N2E2_2603 - hypothetical protein, at 3,060,581 to 3,060,763
_2603
Pf6N2E2_2604 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,060,847 to 3,062,139
_2604
Pf6N2E2_2605 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 3,062,223 to 3,063,104
_2605
Pf6N2E2_2606 - hypothetical protein, at 3,063,087 to 3,063,332
_2606
Position (kb)
3060
3061
3062
3063 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3059.899 kb on + strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3059.900 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.059 kb on - strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.118 kb on + strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.119 kb on - strand, within Pf6N2E2_2602 at 3060.172 kb on + strand, within Pf6N2E2_2602 at 3060.173 kb on - strand, within Pf6N2E2_2602 at 3060.317 kb on + strand at 3060.323 kb on + strand at 3060.393 kb on + strand at 3060.394 kb on - strand at 3060.407 kb on - strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.409 kb on + strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.410 kb on - strand at 3060.413 kb on + strand at 3060.414 kb on - strand at 3060.414 kb on - strand at 3060.437 kb on + strand at 3060.437 kb on + strand at 3060.505 kb on - strand at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.622 kb on - strand, within Pf6N2E2_2603 at 3060.631 kb on - strand, within Pf6N2E2_2603 at 3060.671 kb on + strand, within Pf6N2E2_2603 at 3060.671 kb on + strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.672 kb on - strand, within Pf6N2E2_2603 at 3060.686 kb on + strand, within Pf6N2E2_2603 at 3060.686 kb on + strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.687 kb on - strand, within Pf6N2E2_2603 at 3060.858 kb on - strand at 3060.858 kb on - strand at 3060.962 kb on + strand at 3060.962 kb on + strand at 3060.963 kb on - strand at 3061.174 kb on + strand, within Pf6N2E2_2604 at 3061.174 kb on + strand, within Pf6N2E2_2604 at 3061.175 kb on - strand, within Pf6N2E2_2604 at 3061.175 kb on - strand, within Pf6N2E2_2604 at 3061.217 kb on + strand, within Pf6N2E2_2604 at 3061.217 kb on + strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.218 kb on - strand, within Pf6N2E2_2604 at 3061.296 kb on - strand, within Pf6N2E2_2604 at 3061.347 kb on + strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.348 kb on - strand, within Pf6N2E2_2604 at 3061.414 kb on - strand, within Pf6N2E2_2604 at 3061.498 kb on - strand, within Pf6N2E2_2604 at 3061.682 kb on + strand, within Pf6N2E2_2604 at 3061.682 kb on + strand, within Pf6N2E2_2604 at 3061.683 kb on - strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.754 kb on + strand, within Pf6N2E2_2604 at 3061.911 kb on - strand, within Pf6N2E2_2604 at 3061.911 kb on - strand, within Pf6N2E2_2604 at 3061.911 kb on - strand, within Pf6N2E2_2604 at 3061.998 kb on + strand, within Pf6N2E2_2604 at 3061.999 kb on - strand, within Pf6N2E2_2604 at 3062.107 kb on + strand at 3062.108 kb on - strand at 3062.170 kb on + strand at 3062.183 kb on + strand at 3062.263 kb on - strand at 3062.337 kb on - strand, within Pf6N2E2_2605 at 3062.337 kb on - strand, within Pf6N2E2_2605 at 3062.785 kb on + strand, within Pf6N2E2_2605 at 3062.786 kb on - strand, within Pf6N2E2_2605 at 3062.786 kb on - strand, within Pf6N2E2_2605 at 3062.795 kb on - strand, within Pf6N2E2_2605 at 3062.795 kb on - strand, within Pf6N2E2_2605 at 3062.800 kb on + strand, within Pf6N2E2_2605 at 3062.801 kb on - strand, within Pf6N2E2_2605 at 3062.822 kb on + strand, within Pf6N2E2_2605 at 3062.823 kb on - strand, within Pf6N2E2_2605 at 3062.823 kb on - strand, within Pf6N2E2_2605 at 3062.823 kb on - strand, within Pf6N2E2_2605 at 3062.844 kb on - strand, within Pf6N2E2_2605 at 3063.094 kb on + strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.095 kb on - strand at 3063.122 kb on - strand, within Pf6N2E2_2606 at 3063.122 kb on - strand, within Pf6N2E2_2606 at 3063.138 kb on + strand, within Pf6N2E2_2606 at 3063.139 kb on - strand, within Pf6N2E2_2606
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 3,059,899 + Pf6N2E2_2602 0.49 +0.9 3,059,900 - Pf6N2E2_2602 0.49 +0.4 3,059,900 - Pf6N2E2_2602 0.49 +0.6 3,059,900 - Pf6N2E2_2602 0.49 -0.0 3,059,900 - Pf6N2E2_2602 0.49 +0.2 3,059,900 - Pf6N2E2_2602 0.49 +0.1 3,059,900 - Pf6N2E2_2602 0.49 -0.3 3,060,059 - Pf6N2E2_2602 0.68 +0.6 3,060,059 - Pf6N2E2_2602 0.68 +0.4 3,060,059 - Pf6N2E2_2602 0.68 +0.7 3,060,118 + Pf6N2E2_2602 0.75 +0.5 3,060,118 + Pf6N2E2_2602 0.75 +1.5 3,060,119 - Pf6N2E2_2602 0.75 -0.5 3,060,119 - Pf6N2E2_2602 0.75 +1.3 3,060,119 - Pf6N2E2_2602 0.75 -1.0 3,060,172 + Pf6N2E2_2602 0.81 +0.5 3,060,173 - Pf6N2E2_2602 0.81 -0.4 3,060,317 + -0.9 3,060,323 + +0.0 3,060,393 + -0.7 3,060,394 - -0.2 3,060,407 - -0.2 3,060,409 + +0.3 3,060,409 + +0.8 3,060,409 + -1.3 3,060,410 - +0.4 3,060,410 - +1.0 3,060,410 - +0.6 3,060,413 + +0.6 3,060,414 - -1.3 3,060,414 - +0.6 3,060,437 + +1.0 3,060,437 + +2.0 3,060,505 - +0.4 3,060,622 - Pf6N2E2_2603 0.22 -2.0 3,060,622 - Pf6N2E2_2603 0.22 -0.1 3,060,622 - Pf6N2E2_2603 0.22 -1.1 3,060,631 - Pf6N2E2_2603 0.27 -0.1 3,060,671 + Pf6N2E2_2603 0.49 +1.4 3,060,671 + Pf6N2E2_2603 0.49 +0.5 3,060,672 - Pf6N2E2_2603 0.50 +0.5 3,060,672 - Pf6N2E2_2603 0.50 -0.1 3,060,672 - Pf6N2E2_2603 0.50 -0.5 3,060,672 - Pf6N2E2_2603 0.50 +1.4 3,060,672 - Pf6N2E2_2603 0.50 +0.5 3,060,686 + Pf6N2E2_2603 0.57 +0.1 3,060,686 + Pf6N2E2_2603 0.57 +0.6 3,060,687 - Pf6N2E2_2603 0.58 -0.7 3,060,687 - Pf6N2E2_2603 0.58 +0.8 3,060,687 - Pf6N2E2_2603 0.58 +0.1 3,060,687 - Pf6N2E2_2603 0.58 +0.0 3,060,687 - Pf6N2E2_2603 0.58 +0.6 3,060,858 - +0.1 3,060,858 - +1.7 3,060,962 + -3.0 3,060,962 + +1.1 3,060,963 - +0.2 3,061,174 + Pf6N2E2_2604 0.25 -0.2 3,061,174 + Pf6N2E2_2604 0.25 -1.4 3,061,175 - Pf6N2E2_2604 0.25 +0.4 3,061,175 - Pf6N2E2_2604 0.25 +0.0 3,061,217 + Pf6N2E2_2604 0.29 +0.6 3,061,217 + Pf6N2E2_2604 0.29 -1.1 3,061,218 - Pf6N2E2_2604 0.29 +0.5 3,061,218 - Pf6N2E2_2604 0.29 +0.6 3,061,218 - Pf6N2E2_2604 0.29 +0.9 3,061,296 - Pf6N2E2_2604 0.35 -0.5 3,061,347 + Pf6N2E2_2604 0.39 +0.6 3,061,348 - Pf6N2E2_2604 0.39 -0.7 3,061,348 - Pf6N2E2_2604 0.39 +0.1 3,061,348 - Pf6N2E2_2604 0.39 +0.5 3,061,348 - Pf6N2E2_2604 0.39 +0.3 3,061,414 - Pf6N2E2_2604 0.44 +0.6 3,061,498 - Pf6N2E2_2604 0.50 +0.5 3,061,682 + Pf6N2E2_2604 0.65 -0.6 3,061,682 + Pf6N2E2_2604 0.65 -0.3 3,061,683 - Pf6N2E2_2604 0.65 +0.6 3,061,754 + Pf6N2E2_2604 0.70 +0.0 3,061,754 + Pf6N2E2_2604 0.70 +0.0 3,061,754 + Pf6N2E2_2604 0.70 -0.0 3,061,911 - Pf6N2E2_2604 0.82 +0.8 3,061,911 - Pf6N2E2_2604 0.82 +1.4 3,061,911 - Pf6N2E2_2604 0.82 +0.5 3,061,998 + Pf6N2E2_2604 0.89 -0.0 3,061,999 - Pf6N2E2_2604 0.89 -0.1 3,062,107 + +0.0 3,062,108 - +1.4 3,062,170 + -0.2 3,062,183 + +1.0 3,062,263 - -0.4 3,062,337 - Pf6N2E2_2605 0.13 -1.3 3,062,337 - Pf6N2E2_2605 0.13 -1.5 3,062,785 + Pf6N2E2_2605 0.64 -1.3 3,062,786 - Pf6N2E2_2605 0.64 +1.4 3,062,786 - Pf6N2E2_2605 0.64 +0.0 3,062,795 - Pf6N2E2_2605 0.65 -1.3 3,062,795 - Pf6N2E2_2605 0.65 -2.7 3,062,800 + Pf6N2E2_2605 0.65 -1.6 3,062,801 - Pf6N2E2_2605 0.66 -0.2 3,062,822 + Pf6N2E2_2605 0.68 -0.2 3,062,823 - Pf6N2E2_2605 0.68 -3.4 3,062,823 - Pf6N2E2_2605 0.68 +0.8 3,062,823 - Pf6N2E2_2605 0.68 -0.2 3,062,844 - Pf6N2E2_2605 0.70 +0.6 3,063,094 + +0.0 3,063,095 - +0.0 3,063,095 - +0.0 3,063,095 - -0.4 3,063,095 - +0.3 3,063,095 - -1.9 3,063,122 - Pf6N2E2_2606 0.14 -0.8 3,063,122 - Pf6N2E2_2606 0.14 -0.9 3,063,138 + Pf6N2E2_2606 0.21 -0.3 3,063,139 - Pf6N2E2_2606 0.21 +0.3
Or see this region's nucleotide sequence