Experiment: Cytosine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1827 and Pf6N2E2_1828 are separated by 26 nucleotides Pf6N2E2_1828 and Pf6N2E2_1829 are separated by 479 nucleotides
Pf6N2E2_1827 - Choline-sulfatase (EC 3.1.6.6), at 2,162,920 to 2,164,599
_1827
Pf6N2E2_1828 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 2,164,626 to 2,166,020
_1828
Pf6N2E2_1829 - Mesaconyl-CoA C1-C4 CoA transferase, at 2,166,500 to 2,167,717
_1829
Position (kb)
2164
2165
2166
2167 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.633 kb on + strand, within Pf6N2E2_1827 at 2163.634 kb on - strand, within Pf6N2E2_1827 at 2163.702 kb on - strand, within Pf6N2E2_1827 at 2163.765 kb on + strand, within Pf6N2E2_1827 at 2163.765 kb on + strand, within Pf6N2E2_1827 at 2163.766 kb on - strand, within Pf6N2E2_1827 at 2163.843 kb on - strand, within Pf6N2E2_1827 at 2163.890 kb on - strand, within Pf6N2E2_1827 at 2163.957 kb on - strand, within Pf6N2E2_1827 at 2163.975 kb on + strand, within Pf6N2E2_1827 at 2164.013 kb on - strand, within Pf6N2E2_1827 at 2164.034 kb on + strand, within Pf6N2E2_1827 at 2164.068 kb on + strand, within Pf6N2E2_1827 at 2164.068 kb on + strand, within Pf6N2E2_1827 at 2164.115 kb on + strand, within Pf6N2E2_1827 at 2164.116 kb on - strand, within Pf6N2E2_1827 at 2164.123 kb on - strand, within Pf6N2E2_1827 at 2164.389 kb on - strand, within Pf6N2E2_1827 at 2164.407 kb on + strand, within Pf6N2E2_1827 at 2164.408 kb on - strand, within Pf6N2E2_1827 at 2164.466 kb on - strand at 2164.648 kb on - strand at 2164.648 kb on - strand at 2164.719 kb on - strand at 2164.720 kb on + strand at 2164.720 kb on + strand at 2164.721 kb on - strand at 2164.774 kb on + strand, within Pf6N2E2_1828 at 2164.835 kb on - strand, within Pf6N2E2_1828 at 2164.925 kb on - strand, within Pf6N2E2_1828 at 2164.925 kb on - strand, within Pf6N2E2_1828 at 2164.928 kb on + strand, within Pf6N2E2_1828 at 2164.929 kb on - strand, within Pf6N2E2_1828 at 2164.973 kb on - strand, within Pf6N2E2_1828 at 2164.993 kb on + strand, within Pf6N2E2_1828 at 2164.994 kb on - strand, within Pf6N2E2_1828 at 2164.994 kb on - strand, within Pf6N2E2_1828 at 2165.128 kb on - strand, within Pf6N2E2_1828 at 2165.241 kb on + strand, within Pf6N2E2_1828 at 2165.241 kb on + strand, within Pf6N2E2_1828 at 2165.241 kb on + strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.242 kb on - strand, within Pf6N2E2_1828 at 2165.245 kb on + strand, within Pf6N2E2_1828 at 2165.245 kb on + strand, within Pf6N2E2_1828 at 2165.245 kb on + strand, within Pf6N2E2_1828 at 2165.246 kb on - strand, within Pf6N2E2_1828 at 2165.246 kb on - strand, within Pf6N2E2_1828 at 2165.246 kb on - strand, within Pf6N2E2_1828 at 2165.261 kb on + strand, within Pf6N2E2_1828 at 2165.262 kb on - strand, within Pf6N2E2_1828 at 2165.262 kb on - strand, within Pf6N2E2_1828 at 2165.262 kb on - strand, within Pf6N2E2_1828 at 2165.287 kb on - strand, within Pf6N2E2_1828 at 2165.358 kb on + strand, within Pf6N2E2_1828 at 2165.391 kb on - strand, within Pf6N2E2_1828 at 2165.419 kb on + strand, within Pf6N2E2_1828 at 2165.419 kb on + strand, within Pf6N2E2_1828 at 2165.419 kb on + strand, within Pf6N2E2_1828 at 2165.423 kb on - strand, within Pf6N2E2_1828 at 2165.423 kb on - strand, within Pf6N2E2_1828 at 2165.473 kb on - strand, within Pf6N2E2_1828 at 2165.532 kb on + strand, within Pf6N2E2_1828 at 2165.532 kb on + strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.533 kb on - strand, within Pf6N2E2_1828 at 2165.591 kb on - strand, within Pf6N2E2_1828 at 2165.591 kb on - strand, within Pf6N2E2_1828 at 2165.632 kb on + strand, within Pf6N2E2_1828 at 2165.704 kb on + strand, within Pf6N2E2_1828 at 2165.704 kb on + strand, within Pf6N2E2_1828 at 2165.704 kb on + strand, within Pf6N2E2_1828 at 2165.705 kb on - strand, within Pf6N2E2_1828 at 2165.706 kb on + strand, within Pf6N2E2_1828 at 2165.706 kb on + strand, within Pf6N2E2_1828 at 2165.707 kb on - strand, within Pf6N2E2_1828 at 2165.716 kb on - strand, within Pf6N2E2_1828 at 2165.799 kb on - strand, within Pf6N2E2_1828 at 2165.800 kb on + strand, within Pf6N2E2_1828 at 2165.801 kb on - strand, within Pf6N2E2_1828 at 2165.828 kb on - strand, within Pf6N2E2_1828 at 2165.871 kb on - strand, within Pf6N2E2_1828 at 2165.871 kb on - strand, within Pf6N2E2_1828 at 2165.873 kb on - strand, within Pf6N2E2_1828 at 2165.931 kb on - strand at 2165.933 kb on + strand at 2165.990 kb on - strand at 2166.020 kb on + strand at 2166.020 kb on + strand at 2166.021 kb on - strand at 2166.038 kb on - strand at 2166.054 kb on + strand at 2166.055 kb on - strand at 2166.081 kb on - strand at 2166.106 kb on + strand at 2166.107 kb on - strand at 2166.202 kb on - strand at 2166.341 kb on - strand at 2166.341 kb on - strand at 2166.412 kb on + strand at 2166.464 kb on + strand at 2166.464 kb on + strand at 2166.464 kb on + strand at 2166.464 kb on + strand at 2166.464 kb on + strand at 2166.464 kb on + strand at 2166.464 kb on + strand at 2166.465 kb on - strand at 2166.465 kb on - strand at 2166.468 kb on + strand at 2166.468 kb on + strand at 2166.502 kb on - strand at 2166.669 kb on - strand, within Pf6N2E2_1829 at 2166.804 kb on - strand, within Pf6N2E2_1829 at 2166.902 kb on + strand, within Pf6N2E2_1829 at 2166.902 kb on + strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.903 kb on - strand, within Pf6N2E2_1829 at 2166.938 kb on - strand, within Pf6N2E2_1829
Per-strain Table
Position Strand Gene LocusTag Fraction Cytosine (N) remove 2,163,633 + Pf6N2E2_1827 0.42 +0.4 2,163,633 + Pf6N2E2_1827 0.42 -0.4 2,163,633 + Pf6N2E2_1827 0.42 +0.3 2,163,633 + Pf6N2E2_1827 0.42 +0.2 2,163,634 - Pf6N2E2_1827 0.42 +0.7 2,163,702 - Pf6N2E2_1827 0.47 +0.1 2,163,765 + Pf6N2E2_1827 0.50 +0.2 2,163,765 + Pf6N2E2_1827 0.50 -0.4 2,163,766 - Pf6N2E2_1827 0.50 -0.6 2,163,843 - Pf6N2E2_1827 0.55 +0.5 2,163,890 - Pf6N2E2_1827 0.58 -0.2 2,163,957 - Pf6N2E2_1827 0.62 +0.1 2,163,975 + Pf6N2E2_1827 0.63 -0.2 2,164,013 - Pf6N2E2_1827 0.65 +0.3 2,164,034 + Pf6N2E2_1827 0.66 +0.1 2,164,068 + Pf6N2E2_1827 0.68 +1.6 2,164,068 + Pf6N2E2_1827 0.68 +0.9 2,164,115 + Pf6N2E2_1827 0.71 -0.3 2,164,116 - Pf6N2E2_1827 0.71 -0.3 2,164,123 - Pf6N2E2_1827 0.72 -1.5 2,164,389 - Pf6N2E2_1827 0.87 +0.9 2,164,407 + Pf6N2E2_1827 0.89 +2.0 2,164,408 - Pf6N2E2_1827 0.89 +0.9 2,164,466 - -0.9 2,164,648 - -0.6 2,164,648 - -0.4 2,164,719 - +0.4 2,164,720 + -0.4 2,164,720 + -0.7 2,164,721 - +0.1 2,164,774 + Pf6N2E2_1828 0.11 +0.5 2,164,835 - Pf6N2E2_1828 0.15 +0.1 2,164,925 - Pf6N2E2_1828 0.21 -0.5 2,164,925 - Pf6N2E2_1828 0.21 -0.5 2,164,928 + Pf6N2E2_1828 0.22 +0.1 2,164,929 - Pf6N2E2_1828 0.22 -0.6 2,164,973 - Pf6N2E2_1828 0.25 -1.9 2,164,993 + Pf6N2E2_1828 0.26 +0.1 2,164,994 - Pf6N2E2_1828 0.26 +1.0 2,164,994 - Pf6N2E2_1828 0.26 -0.7 2,165,128 - Pf6N2E2_1828 0.36 +0.2 2,165,241 + Pf6N2E2_1828 0.44 +0.1 2,165,241 + Pf6N2E2_1828 0.44 -0.4 2,165,241 + Pf6N2E2_1828 0.44 +1.1 2,165,242 - Pf6N2E2_1828 0.44 -1.9 2,165,242 - Pf6N2E2_1828 0.44 +0.4 2,165,242 - Pf6N2E2_1828 0.44 -0.8 2,165,242 - Pf6N2E2_1828 0.44 -0.2 2,165,245 + Pf6N2E2_1828 0.44 -2.8 2,165,245 + Pf6N2E2_1828 0.44 -0.6 2,165,245 + Pf6N2E2_1828 0.44 -0.4 2,165,246 - Pf6N2E2_1828 0.44 +0.1 2,165,246 - Pf6N2E2_1828 0.44 -0.1 2,165,246 - Pf6N2E2_1828 0.44 +0.4 2,165,261 + Pf6N2E2_1828 0.46 +0.0 2,165,262 - Pf6N2E2_1828 0.46 -0.1 2,165,262 - Pf6N2E2_1828 0.46 -1.9 2,165,262 - Pf6N2E2_1828 0.46 +0.2 2,165,287 - Pf6N2E2_1828 0.47 -0.6 2,165,358 + Pf6N2E2_1828 0.52 +0.3 2,165,391 - Pf6N2E2_1828 0.55 -1.4 2,165,419 + Pf6N2E2_1828 0.57 -0.5 2,165,419 + Pf6N2E2_1828 0.57 +0.1 2,165,419 + Pf6N2E2_1828 0.57 +1.2 2,165,423 - Pf6N2E2_1828 0.57 +0.6 2,165,423 - Pf6N2E2_1828 0.57 +0.1 2,165,473 - Pf6N2E2_1828 0.61 +0.5 2,165,532 + Pf6N2E2_1828 0.65 -0.6 2,165,532 + Pf6N2E2_1828 0.65 +0.6 2,165,533 - Pf6N2E2_1828 0.65 -0.1 2,165,533 - Pf6N2E2_1828 0.65 -0.7 2,165,533 - Pf6N2E2_1828 0.65 -0.1 2,165,533 - Pf6N2E2_1828 0.65 -0.7 2,165,591 - Pf6N2E2_1828 0.69 -0.0 2,165,591 - Pf6N2E2_1828 0.69 -0.4 2,165,632 + Pf6N2E2_1828 0.72 -0.4 2,165,704 + Pf6N2E2_1828 0.77 -1.9 2,165,704 + Pf6N2E2_1828 0.77 +0.6 2,165,704 + Pf6N2E2_1828 0.77 -0.7 2,165,705 - Pf6N2E2_1828 0.77 +0.9 2,165,706 + Pf6N2E2_1828 0.77 +0.4 2,165,706 + Pf6N2E2_1828 0.77 +0.1 2,165,707 - Pf6N2E2_1828 0.77 -0.2 2,165,716 - Pf6N2E2_1828 0.78 +0.3 2,165,799 - Pf6N2E2_1828 0.84 +1.2 2,165,800 + Pf6N2E2_1828 0.84 -1.7 2,165,801 - Pf6N2E2_1828 0.84 -1.6 2,165,828 - Pf6N2E2_1828 0.86 +0.4 2,165,871 - Pf6N2E2_1828 0.89 +0.1 2,165,871 - Pf6N2E2_1828 0.89 -0.8 2,165,873 - Pf6N2E2_1828 0.89 -4.6 2,165,931 - -1.5 2,165,933 + -0.9 2,165,990 - -0.8 2,166,020 + -3.1 2,166,020 + -0.7 2,166,021 - -0.1 2,166,038 - -0.7 2,166,054 + +1.8 2,166,055 - -0.6 2,166,081 - +0.2 2,166,106 + -0.3 2,166,107 - -2.1 2,166,202 - -0.7 2,166,341 - +1.4 2,166,341 - +0.1 2,166,412 + +0.5 2,166,464 + +1.5 2,166,464 + -1.1 2,166,464 + -0.5 2,166,464 + -0.6 2,166,464 + +0.6 2,166,464 + -1.3 2,166,464 + +0.2 2,166,465 - -0.4 2,166,465 - +1.0 2,166,468 + -0.3 2,166,468 + +1.0 2,166,502 - +0.2 2,166,669 - Pf6N2E2_1829 0.14 -0.2 2,166,804 - Pf6N2E2_1829 0.25 -0.6 2,166,902 + Pf6N2E2_1829 0.33 -0.4 2,166,902 + Pf6N2E2_1829 0.33 +0.1 2,166,903 - Pf6N2E2_1829 0.33 +0.1 2,166,903 - Pf6N2E2_1829 0.33 +0.2 2,166,903 - Pf6N2E2_1829 0.33 -0.6 2,166,903 - Pf6N2E2_1829 0.33 -0.7 2,166,938 - Pf6N2E2_1829 0.36 +0.5
Or see this region's nucleotide sequence