Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27545 and BWI76_RS27550 are separated by 159 nucleotides BWI76_RS27550 and BWI76_RS27555 overlap by 20 nucleotides BWI76_RS27555 and BWI76_RS27560 are separated by 50 nucleotides BWI76_RS27560 and BWI76_RS27565 are separated by 80 nucleotides
BWI76_RS27545: BWI76_RS27545 - sugar isomerase, at 5,718,272 to 5,719,003
_RS27545
BWI76_RS27550: BWI76_RS27550 - hypothetical protein, at 5,719,163 to 5,719,837
_RS27550
BWI76_RS27555: BWI76_RS27555 - putative sugar kinase/transcriptional regulator, ATPase domain protein, at 5,719,818 to 5,720,750
_RS27555
BWI76_RS27560: BWI76_RS27560 - hypothetical protein, at 5,720,801 to 5,721,661
_RS27560
BWI76_RS27565: BWI76_RS27565 - sugar aldolase, at 5,721,742 to 5,722,593
_RS27565
Position (kb) 5719
5720
5721 Strain fitness (log2 ratio) -1
0
1
2
3 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.881 kb on - strand, within BWI76_RS27545 at 5718.902 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5718.927 kb on - strand, within BWI76_RS27545 at 5719.020 kb on - strand at 5719.150 kb on + strand at 5719.150 kb on + strand at 5719.151 kb on + strand at 5719.169 kb on + strand at 5719.176 kb on - strand at 5719.177 kb on - strand at 5719.177 kb on - strand at 5719.466 kb on - strand, within BWI76_RS27550 at 5719.481 kb on - strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.580 kb on + strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.590 kb on - strand, within BWI76_RS27550 at 5719.789 kb on - strand at 5719.796 kb on - strand at 5719.992 kb on - strand, within BWI76_RS27555 at 5720.129 kb on + strand, within BWI76_RS27555 at 5720.144 kb on + strand, within BWI76_RS27555 at 5720.152 kb on - strand, within BWI76_RS27555 at 5720.164 kb on - strand, within BWI76_RS27555 at 5720.240 kb on + strand, within BWI76_RS27555 at 5720.245 kb on + strand at 5720.245 kb on + strand, within BWI76_RS27555 at 5720.253 kb on - strand, within BWI76_RS27555 at 5720.253 kb on - strand, within BWI76_RS27555 at 5720.260 kb on + strand, within BWI76_RS27555 at 5720.278 kb on + strand, within BWI76_RS27555 at 5720.335 kb on + strand, within BWI76_RS27555 at 5720.511 kb on - strand, within BWI76_RS27555 at 5720.548 kb on - strand, within BWI76_RS27555 at 5720.548 kb on - strand, within BWI76_RS27555 at 5720.607 kb on + strand, within BWI76_RS27555 at 5720.613 kb on + strand, within BWI76_RS27555 at 5720.700 kb on + strand at 5720.755 kb on + strand at 5720.803 kb on + strand at 5720.804 kb on + strand at 5720.807 kb on - strand at 5720.811 kb on - strand at 5720.899 kb on + strand, within BWI76_RS27560 at 5721.270 kb on + strand, within BWI76_RS27560 at 5721.325 kb on - strand, within BWI76_RS27560 at 5721.371 kb on + strand, within BWI76_RS27560 at 5721.371 kb on + strand, within BWI76_RS27560 at 5721.388 kb on - strand, within BWI76_RS27560 at 5721.393 kb on - strand, within BWI76_RS27560 at 5721.447 kb on + strand, within BWI76_RS27560 at 5721.457 kb on + strand, within BWI76_RS27560 at 5721.579 kb on - strand at 5721.579 kb on - strand at 5721.693 kb on - strand at 5721.708 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM remove 5,718,881 - BWI76_RS27545 0.83 -0.4 5,718,881 - BWI76_RS27545 0.83 +1.7 5,718,902 - BWI76_RS27545 0.86 -0.0 5,718,927 - BWI76_RS27545 0.89 +3.3 5,718,927 - BWI76_RS27545 0.89 -0.1 5,718,927 - BWI76_RS27545 0.89 +0.2 5,718,927 - BWI76_RS27545 0.89 -0.2 5,719,020 - +0.4 5,719,150 + +0.0 5,719,150 + -0.1 5,719,151 + -0.9 5,719,169 + -0.8 5,719,176 - +0.0 5,719,177 - -0.7 5,719,177 - +0.1 5,719,466 - BWI76_RS27550 0.45 +0.6 5,719,481 - BWI76_RS27550 0.47 +0.1 5,719,580 + BWI76_RS27550 0.62 -0.1 5,719,580 + BWI76_RS27550 0.62 -0.3 5,719,590 - BWI76_RS27550 0.63 -0.5 5,719,590 - BWI76_RS27550 0.63 -0.3 5,719,789 - +0.1 5,719,796 - +0.1 5,719,992 - BWI76_RS27555 0.19 -1.0 5,720,129 + BWI76_RS27555 0.33 -1.1 5,720,144 + BWI76_RS27555 0.35 -0.1 5,720,152 - BWI76_RS27555 0.36 -1.4 5,720,164 - BWI76_RS27555 0.37 -0.4 5,720,240 + BWI76_RS27555 0.45 +0.7 5,720,245 + +0.1 5,720,245 + BWI76_RS27555 0.46 -0.7 5,720,253 - BWI76_RS27555 0.47 +1.0 5,720,253 - BWI76_RS27555 0.47 -1.0 5,720,260 + BWI76_RS27555 0.47 -0.3 5,720,278 + BWI76_RS27555 0.49 -1.0 5,720,335 + BWI76_RS27555 0.55 -0.2 5,720,511 - BWI76_RS27555 0.74 -1.0 5,720,548 - BWI76_RS27555 0.78 -1.2 5,720,548 - BWI76_RS27555 0.78 +1.4 5,720,607 + BWI76_RS27555 0.85 -0.3 5,720,613 + BWI76_RS27555 0.85 -1.4 5,720,700 + +0.0 5,720,755 + -0.5 5,720,803 + -0.6 5,720,804 + -0.8 5,720,807 - -0.2 5,720,811 - -0.0 5,720,899 + BWI76_RS27560 0.11 +2.0 5,721,270 + BWI76_RS27560 0.54 -1.3 5,721,325 - BWI76_RS27560 0.61 +0.6 5,721,371 + BWI76_RS27560 0.66 +1.0 5,721,371 + BWI76_RS27560 0.66 -0.6 5,721,388 - BWI76_RS27560 0.68 -0.2 5,721,393 - BWI76_RS27560 0.69 +0.1 5,721,447 + BWI76_RS27560 0.75 +0.4 5,721,457 + BWI76_RS27560 0.76 -0.0 5,721,579 - +0.5 5,721,579 - -0.1 5,721,693 - -0.0 5,721,708 - +0.4
Or see this region's nucleotide sequence