Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16185 and BWI76_RS16190 are separated by 138 nucleotides BWI76_RS16190 and BWI76_RS16195 are separated by 127 nucleotides BWI76_RS16195 and BWI76_RS16200 are separated by 145 nucleotides
BWI76_RS16185: BWI76_RS16185 - transcriptional regulator FNR, at 3,358,584 to 3,359,336
_RS16185
BWI76_RS16190: BWI76_RS16190 - universal stress protein UspE, at 3,359,475 to 3,360,425
_RS16190
BWI76_RS16195: BWI76_RS16195 - hypothetical protein, at 3,360,553 to 3,360,741
_RS16195
BWI76_RS16200: BWI76_RS16200 - NAD(P) transhydrogenase subunit beta, at 3,360,887 to 3,362,275
_RS16200
Position (kb)
3359
3360
3361 Strain fitness (log2 ratio)
-1
0
1
2 at 3358.490 kb on - strand at 3358.519 kb on + strand at 3359.313 kb on + strand at 3359.397 kb on - strand at 3359.465 kb on - strand at 3359.465 kb on - strand at 3359.512 kb on - strand at 3359.640 kb on - strand, within BWI76_RS16190 at 3359.640 kb on - strand, within BWI76_RS16190 at 3359.753 kb on - strand, within BWI76_RS16190 at 3360.571 kb on - strand at 3360.590 kb on + strand, within BWI76_RS16195 at 3360.681 kb on - strand, within BWI76_RS16195 at 3360.892 kb on - strand at 3361.117 kb on + strand, within BWI76_RS16200
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM remove 3,358,490 - -0.6 3,358,519 + -0.1 3,359,313 + +0.0 3,359,397 - -0.3 3,359,465 - +0.4 3,359,465 - +2.2 3,359,512 - +0.5 3,359,640 - BWI76_RS16190 0.17 +1.0 3,359,640 - BWI76_RS16190 0.17 -0.1 3,359,753 - BWI76_RS16190 0.29 +0.5 3,360,571 - +0.2 3,360,590 + BWI76_RS16195 0.20 -0.6 3,360,681 - BWI76_RS16195 0.68 +0.7 3,360,892 - -0.6 3,361,117 + BWI76_RS16200 0.17 -0.3
Or see this region's nucleotide sequence