Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13040 and BWI76_RS13045 are separated by 161 nucleotides BWI76_RS13045 and BWI76_RS13050 are separated by 148 nucleotides BWI76_RS13050 and BWI76_RS13055 are separated by 128 nucleotides
BWI76_RS13040: BWI76_RS13040 - transcriptional regulator, at 2,721,182 to 2,721,985
_RS13040
BWI76_RS13045: BWI76_RS13045 - hypothetical protein, at 2,722,147 to 2,722,542
_RS13045
BWI76_RS13050: BWI76_RS13050 - transcriptional regulator, at 2,722,691 to 2,723,125
_RS13050
BWI76_RS13055: BWI76_RS13055 - NmrA family protein, at 2,723,254 to 2,724,165
_RS13055
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-1
0
1 at 2721.696 kb on + strand, within BWI76_RS13040 at 2721.718 kb on - strand, within BWI76_RS13040 at 2721.776 kb on - strand, within BWI76_RS13040 at 2722.076 kb on - strand at 2722.084 kb on + strand at 2722.193 kb on - strand, within BWI76_RS13045 at 2722.193 kb on - strand, within BWI76_RS13045 at 2722.350 kb on - strand, within BWI76_RS13045 at 2722.350 kb on - strand, within BWI76_RS13045 at 2722.356 kb on + strand, within BWI76_RS13045 at 2722.393 kb on + strand, within BWI76_RS13045 at 2722.416 kb on - strand, within BWI76_RS13045 at 2722.472 kb on - strand, within BWI76_RS13045 at 2722.590 kb on + strand at 2722.672 kb on + strand at 2722.673 kb on + strand at 2722.701 kb on + strand at 2722.920 kb on - strand, within BWI76_RS13050 at 2723.196 kb on + strand at 2723.535 kb on - strand, within BWI76_RS13055 at 2723.740 kb on + strand, within BWI76_RS13055 at 2723.740 kb on + strand, within BWI76_RS13055 at 2723.809 kb on + strand, within BWI76_RS13055 at 2724.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM remove 2,721,696 + BWI76_RS13040 0.64 +0.5 2,721,718 - BWI76_RS13040 0.67 -0.8 2,721,776 - BWI76_RS13040 0.74 +0.2 2,722,076 - -0.5 2,722,084 + -1.5 2,722,193 - BWI76_RS13045 0.12 +0.8 2,722,193 - BWI76_RS13045 0.12 -0.1 2,722,350 - BWI76_RS13045 0.51 +0.1 2,722,350 - BWI76_RS13045 0.51 +0.4 2,722,356 + BWI76_RS13045 0.53 -0.0 2,722,393 + BWI76_RS13045 0.62 -0.8 2,722,416 - BWI76_RS13045 0.68 -0.5 2,722,472 - BWI76_RS13045 0.82 +0.6 2,722,590 + -0.3 2,722,672 + +0.3 2,722,673 + -1.4 2,722,701 + +0.5 2,722,920 - BWI76_RS13050 0.53 +0.5 2,723,196 + -0.2 2,723,535 - BWI76_RS13055 0.31 +0.0 2,723,740 + BWI76_RS13055 0.53 +0.3 2,723,740 + BWI76_RS13055 0.53 +0.1 2,723,809 + BWI76_RS13055 0.61 +0.1 2,724,098 - +0.4
Or see this region's nucleotide sequence