Experiment: Varel_Bryant_medium_Glucose with Cefoxitin 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2019 and BT2020 are separated by 72 nucleotides BT2020 and BT2021 are separated by 658 nucleotides
BT2019: BT2019 - polyphosphate kinase (NCBI ptt file), at 2,532,821 to 2,534,899
BT2019
BT2020: BT2020 - putative phosphate/sulphate permeases (NCBI ptt file), at 2,534,972 to 2,537,224
BT2020
BT2021: BT2021 - conserved hypothetical protein, putative non-specific DNA-binding protein (NCBI ptt file), at 2,537,883 to 2,538,410
BT2021
Position (kb)
2534
2535
2536
2537
2538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2534.019 kb on + strand, within BT2019 at 2534.020 kb on - strand, within BT2019 at 2534.020 kb on - strand, within BT2019 at 2534.121 kb on + strand, within BT2019 at 2534.186 kb on + strand, within BT2019 at 2534.234 kb on + strand, within BT2019 at 2534.286 kb on - strand, within BT2019 at 2534.425 kb on + strand, within BT2019 at 2534.426 kb on - strand, within BT2019 at 2534.426 kb on - strand, within BT2019 at 2534.553 kb on - strand, within BT2019 at 2534.616 kb on - strand, within BT2019 at 2534.616 kb on - strand, within BT2019 at 2534.905 kb on - strand at 2534.958 kb on - strand at 2534.985 kb on - strand at 2534.989 kb on - strand at 2534.989 kb on - strand at 2535.171 kb on + strand at 2535.171 kb on + strand at 2535.172 kb on - strand at 2535.207 kb on - strand, within BT2020 at 2535.227 kb on - strand, within BT2020 at 2535.227 kb on - strand, within BT2020 at 2535.228 kb on + strand, within BT2020 at 2535.229 kb on - strand, within BT2020 at 2535.466 kb on + strand, within BT2020 at 2535.471 kb on + strand, within BT2020 at 2535.471 kb on + strand, within BT2020 at 2535.472 kb on - strand, within BT2020 at 2535.509 kb on + strand, within BT2020 at 2535.832 kb on - strand, within BT2020 at 2535.836 kb on + strand, within BT2020 at 2535.836 kb on + strand, within BT2020 at 2536.268 kb on + strand, within BT2020 at 2536.268 kb on + strand, within BT2020 at 2536.269 kb on - strand, within BT2020 at 2536.285 kb on - strand, within BT2020 at 2536.289 kb on + strand, within BT2020 at 2536.508 kb on - strand, within BT2020 at 2536.811 kb on + strand, within BT2020 at 2536.814 kb on + strand, within BT2020 at 2536.814 kb on + strand, within BT2020 at 2536.961 kb on + strand, within BT2020 at 2536.962 kb on - strand, within BT2020 at 2537.115 kb on + strand at 2537.115 kb on + strand at 2537.120 kb on - strand at 2537.165 kb on + strand at 2537.166 kb on - strand at 2537.188 kb on - strand at 2537.278 kb on - strand at 2537.286 kb on - strand at 2537.290 kb on - strand at 2537.399 kb on - strand at 2537.407 kb on - strand at 2537.455 kb on + strand at 2537.456 kb on - strand at 2537.456 kb on - strand at 2537.460 kb on + strand at 2537.475 kb on + strand at 2537.476 kb on - strand at 2537.517 kb on + strand at 2537.566 kb on + strand at 2537.576 kb on + strand at 2537.580 kb on + strand at 2537.581 kb on - strand at 2537.584 kb on - strand at 2537.588 kb on + strand at 2537.595 kb on - strand at 2537.598 kb on + strand at 2537.638 kb on + strand at 2537.643 kb on + strand at 2537.644 kb on - strand at 2537.644 kb on - strand at 2537.703 kb on + strand at 2537.704 kb on - strand at 2537.792 kb on - strand at 2537.805 kb on - strand at 2537.837 kb on - strand at 2537.859 kb on + strand at 2538.021 kb on + strand, within BT2021 at 2538.193 kb on - strand, within BT2021
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Cefoxitin 0.025 mM remove 2,534,019 + BT2019 0.58 -0.9 2,534,020 - BT2019 0.58 -0.6 2,534,020 - BT2019 0.58 +0.0 2,534,121 + BT2019 0.63 -1.5 2,534,186 + BT2019 0.66 +0.0 2,534,234 + BT2019 0.68 +1.0 2,534,286 - BT2019 0.70 -1.0 2,534,425 + BT2019 0.77 -2.0 2,534,426 - BT2019 0.77 -1.0 2,534,426 - BT2019 0.77 -0.4 2,534,553 - BT2019 0.83 -0.4 2,534,616 - BT2019 0.86 -0.6 2,534,616 - BT2019 0.86 -1.2 2,534,905 - -1.0 2,534,958 - -1.3 2,534,985 - -1.0 2,534,989 - -2.3 2,534,989 - -0.4 2,535,171 + -0.6 2,535,171 + -1.7 2,535,172 - -1.6 2,535,207 - BT2020 0.10 +0.0 2,535,227 - BT2020 0.11 -0.3 2,535,227 - BT2020 0.11 +0.0 2,535,228 + BT2020 0.11 +0.6 2,535,229 - BT2020 0.11 -0.4 2,535,466 + BT2020 0.22 -1.2 2,535,471 + BT2020 0.22 +0.4 2,535,471 + BT2020 0.22 +0.0 2,535,472 - BT2020 0.22 -0.2 2,535,509 + BT2020 0.24 +0.0 2,535,832 - BT2020 0.38 -0.2 2,535,836 + BT2020 0.38 -0.9 2,535,836 + BT2020 0.38 -0.6 2,536,268 + BT2020 0.58 +0.0 2,536,268 + BT2020 0.58 -1.0 2,536,269 - BT2020 0.58 +2.0 2,536,285 - BT2020 0.58 +0.3 2,536,289 + BT2020 0.58 -2.3 2,536,508 - BT2020 0.68 +2.0 2,536,811 + BT2020 0.82 +0.3 2,536,814 + BT2020 0.82 -1.0 2,536,814 + BT2020 0.82 -0.4 2,536,961 + BT2020 0.88 -1.4 2,536,962 - BT2020 0.88 -1.6 2,537,115 + +0.0 2,537,115 + -2.8 2,537,120 - -0.3 2,537,165 + -1.5 2,537,166 - +0.3 2,537,188 - +0.6 2,537,278 - +0.0 2,537,286 - -1.0 2,537,290 - -0.8 2,537,399 - -2.8 2,537,407 - +0.0 2,537,455 + -0.6 2,537,456 - +1.0 2,537,456 - +0.0 2,537,460 + -0.8 2,537,475 + -0.3 2,537,476 - +0.6 2,537,517 + -0.3 2,537,566 + +1.0 2,537,576 + -1.0 2,537,580 + -0.3 2,537,581 - -0.9 2,537,584 - -2.0 2,537,588 + -0.4 2,537,595 - -2.0 2,537,598 + -2.0 2,537,638 + -0.6 2,537,643 + +0.0 2,537,644 - -2.3 2,537,644 - +3.2 2,537,703 + -0.7 2,537,704 - -2.0 2,537,792 - -1.7 2,537,805 - +0.4 2,537,837 - -1.2 2,537,859 + -2.0 2,538,021 + BT2021 0.26 -3.2 2,538,193 - BT2021 0.59 -1.3
Or see this region's nucleotide sequence