Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16900 and BWI76_RS16905 overlap by 4 nucleotides BWI76_RS16905 and BWI76_RS16910 overlap by 8 nucleotides BWI76_RS16910 and BWI76_RS16915 are separated by 9 nucleotides
BWI76_RS16900: BWI76_RS16900 - 4-hydroxy-2-oxovalerate aldolase, at 3,504,598 to 3,505,614
_RS16900
BWI76_RS16905: BWI76_RS16905 - acetaldehyde dehydrogenase (acetylating), at 3,505,611 to 3,506,561
_RS16905
BWI76_RS16910: BWI76_RS16910 - 2-keto-4-pentenoate hydratase, at 3,506,554 to 3,507,360
_RS16910
BWI76_RS16915: BWI76_RS16915 - alpha/beta hydrolase fold protein, at 3,507,370 to 3,508,236
_RS16915
Position (kb)
3505
3506
3507 Strain fitness (log2 ratio)
-1
0
1
2 at 3504.836 kb on + strand, within BWI76_RS16900 at 3505.512 kb on - strand, within BWI76_RS16900 at 3506.110 kb on - strand, within BWI76_RS16905 at 3506.206 kb on + strand, within BWI76_RS16905 at 3506.232 kb on + strand, within BWI76_RS16905 at 3506.287 kb on - strand, within BWI76_RS16905 at 3506.575 kb on - strand at 3506.594 kb on - strand at 3506.986 kb on - strand, within BWI76_RS16910 at 3507.250 kb on - strand, within BWI76_RS16910 at 3507.409 kb on + strand at 3507.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 20mM remove 3,504,836 + BWI76_RS16900 0.23 +0.2 3,505,512 - BWI76_RS16900 0.90 -0.0 3,506,110 - BWI76_RS16905 0.52 +1.3 3,506,206 + BWI76_RS16905 0.63 -0.8 3,506,232 + BWI76_RS16905 0.65 +1.9 3,506,287 - BWI76_RS16905 0.71 +0.0 3,506,575 - +0.1 3,506,594 - -0.8 3,506,986 - BWI76_RS16910 0.54 -1.0 3,507,250 - BWI76_RS16910 0.86 -0.3 3,507,409 + -0.2 3,507,410 - -0.3
Or see this region's nucleotide sequence