Experiment: Varel_Bryant_medium_Glucose with Dimetridazole 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2156 and BT2157 are separated by 139 nucleotides BT2157 and BT2158 are separated by 52 nucleotides
BT2156: BT2156 - putative sugar phosphate isomerase/epimerase (NCBI ptt file), at 2,714,468 to 2,715,382
BT2156
BT2157: BT2157 - 3-keto-alpha-glucoside 1,2-lyase; 3-ketoglucose dehydratase (from data) , at 2,715,522 to 2,716,394
BT2157
BT2158: BT2158 - glycoside 3-dehydrogenase (EC 1.1.99.13) (from data) , at 2,716,447 to 2,717,922
BT2158
Position (kb)
2715
2716
2717 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2714.561 kb on + strand, within BT2156 at 2714.666 kb on + strand, within BT2156 at 2714.700 kb on + strand, within BT2156 at 2714.736 kb on + strand, within BT2156 at 2714.760 kb on + strand, within BT2156 at 2714.773 kb on + strand, within BT2156 at 2714.787 kb on + strand, within BT2156 at 2714.803 kb on + strand, within BT2156 at 2714.804 kb on - strand, within BT2156 at 2714.811 kb on - strand, within BT2156 at 2714.837 kb on - strand, within BT2156 at 2714.837 kb on - strand, within BT2156 at 2714.889 kb on + strand, within BT2156 at 2714.890 kb on - strand, within BT2156 at 2714.901 kb on + strand, within BT2156 at 2714.901 kb on + strand, within BT2156 at 2714.902 kb on - strand, within BT2156 at 2714.902 kb on - strand, within BT2156 at 2714.902 kb on - strand, within BT2156 at 2714.906 kb on - strand, within BT2156 at 2714.927 kb on - strand, within BT2156 at 2714.948 kb on + strand, within BT2156 at 2714.955 kb on + strand, within BT2156 at 2714.956 kb on - strand, within BT2156 at 2714.956 kb on - strand, within BT2156 at 2714.987 kb on - strand, within BT2156 at 2714.987 kb on - strand, within BT2156 at 2715.143 kb on - strand, within BT2156 at 2715.145 kb on + strand, within BT2156 at 2715.147 kb on + strand, within BT2156 at 2715.148 kb on - strand, within BT2156 at 2715.169 kb on + strand, within BT2156 at 2715.202 kb on + strand, within BT2156 at 2715.203 kb on - strand, within BT2156 at 2715.208 kb on - strand, within BT2156 at 2715.233 kb on - strand, within BT2156 at 2715.233 kb on - strand at 2715.233 kb on - strand, within BT2156 at 2715.252 kb on - strand, within BT2156 at 2715.252 kb on - strand, within BT2156 at 2715.254 kb on - strand, within BT2156 at 2715.342 kb on - strand at 2715.406 kb on + strand at 2715.501 kb on - strand at 2715.511 kb on + strand at 2715.826 kb on + strand, within BT2157 at 2715.924 kb on - strand, within BT2157 at 2715.924 kb on - strand, within BT2157 at 2716.207 kb on + strand, within BT2157 at 2716.221 kb on + strand, within BT2157 at 2716.400 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Dimetridazole 0.0125 mM remove 2,714,561 + BT2156 0.10 +0.4 2,714,666 + BT2156 0.22 -0.2 2,714,700 + BT2156 0.25 -3.6 2,714,736 + BT2156 0.29 -1.0 2,714,760 + BT2156 0.32 +1.0 2,714,773 + BT2156 0.33 -2.0 2,714,787 + BT2156 0.35 -1.0 2,714,803 + BT2156 0.37 -2.6 2,714,804 - BT2156 0.37 -2.2 2,714,811 - BT2156 0.37 -1.5 2,714,837 - BT2156 0.40 -1.6 2,714,837 - BT2156 0.40 -2.1 2,714,889 + BT2156 0.46 -2.3 2,714,890 - BT2156 0.46 +2.0 2,714,901 + BT2156 0.47 -2.8 2,714,901 + BT2156 0.47 -4.0 2,714,902 - BT2156 0.47 -2.4 2,714,902 - BT2156 0.47 -1.5 2,714,902 - BT2156 0.47 -1.6 2,714,906 - BT2156 0.48 -1.0 2,714,927 - BT2156 0.50 -0.0 2,714,948 + BT2156 0.52 -1.8 2,714,955 + BT2156 0.53 -0.0 2,714,956 - BT2156 0.53 -2.0 2,714,956 - BT2156 0.53 -2.0 2,714,987 - BT2156 0.57 +0.2 2,714,987 - BT2156 0.57 -1.5 2,715,143 - BT2156 0.74 -1.0 2,715,145 + BT2156 0.74 -3.0 2,715,147 + BT2156 0.74 -0.5 2,715,148 - BT2156 0.74 -0.6 2,715,169 + BT2156 0.77 -1.6 2,715,202 + BT2156 0.80 -2.8 2,715,203 - BT2156 0.80 -3.0 2,715,208 - BT2156 0.81 +0.7 2,715,233 - BT2156 0.84 -0.0 2,715,233 - -1.0 2,715,233 - BT2156 0.84 +1.0 2,715,252 - BT2156 0.86 -4.0 2,715,252 - BT2156 0.86 -1.1 2,715,254 - BT2156 0.86 -4.3 2,715,342 - -2.4 2,715,406 + +0.5 2,715,501 - -1.6 2,715,511 + -1.4 2,715,826 + BT2157 0.35 -1.0 2,715,924 - BT2157 0.46 -2.4 2,715,924 - BT2157 0.46 -3.4 2,716,207 + BT2157 0.78 -2.0 2,716,221 + BT2157 0.80 -3.9 2,716,400 + -0.0
Or see this region's nucleotide sequence