Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_497 and Pf6N2E2_498 are separated by 183 nucleotides Pf6N2E2_498 and Pf6N2E2_499 are separated by 608 nucleotides
Pf6N2E2_497 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 564,022 to 565,389
_497
Pf6N2E2_498 - Dihydropyrimidinase (EC 3.5.2.2) <small>(from data)</small>, at 565,573 to 567,012
_498
Pf6N2E2_499 - Possible pyrimidine permease in reductive pathway, at 567,621 to 569,099
_499
Position (kb) 565
566
567
568 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 564.617 kb on + strand, within Pf6N2E2_497 at 564.617 kb on + strand, within Pf6N2E2_497 at 564.815 kb on + strand, within Pf6N2E2_497 at 564.815 kb on + strand, within Pf6N2E2_497 at 564.833 kb on + strand, within Pf6N2E2_497 at 564.834 kb on - strand, within Pf6N2E2_497 at 564.834 kb on - strand, within Pf6N2E2_497 at 564.834 kb on - strand, within Pf6N2E2_497 at 565.028 kb on + strand, within Pf6N2E2_497 at 565.197 kb on + strand, within Pf6N2E2_497 at 565.198 kb on - strand, within Pf6N2E2_497 at 565.256 kb on + strand at 565.262 kb on + strand at 565.262 kb on + strand at 565.656 kb on + strand at 565.658 kb on + strand at 565.658 kb on + strand at 565.658 kb on + strand at 565.658 kb on + strand at 565.684 kb on + strand at 565.684 kb on + strand at 566.224 kb on + strand, within Pf6N2E2_498 at 566.224 kb on + strand, within Pf6N2E2_498 at 566.234 kb on + strand, within Pf6N2E2_498 at 566.262 kb on - strand, within Pf6N2E2_498 at 566.306 kb on + strand, within Pf6N2E2_498 at 566.549 kb on + strand, within Pf6N2E2_498 at 566.549 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.829 kb on + strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.830 kb on - strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.864 kb on + strand, within Pf6N2E2_498 at 566.865 kb on - strand, within Pf6N2E2_498 at 566.979 kb on + strand at 567.061 kb on + strand at 567.062 kb on - strand at 567.077 kb on + strand at 567.095 kb on + strand at 567.096 kb on - strand at 567.181 kb on - strand at 567.288 kb on + strand at 567.392 kb on - strand at 567.406 kb on + strand at 567.473 kb on + strand at 567.473 kb on + strand at 567.475 kb on + strand at 567.567 kb on + strand at 567.567 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.701 kb on + strand at 567.702 kb on - strand at 567.798 kb on + strand, within Pf6N2E2_499 at 567.798 kb on + strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499 at 567.799 kb on - strand, within Pf6N2E2_499
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 564,617 + Pf6N2E2_497 0.43 -1.0 564,617 + Pf6N2E2_497 0.43 -1.5 564,815 + Pf6N2E2_497 0.58 -1.4 564,815 + Pf6N2E2_497 0.58 -3.5 564,833 + Pf6N2E2_497 0.59 -3.9 564,834 - Pf6N2E2_497 0.59 -5.3 564,834 - Pf6N2E2_497 0.59 -3.6 564,834 - Pf6N2E2_497 0.59 -3.4 565,028 + Pf6N2E2_497 0.74 -3.1 565,197 + Pf6N2E2_497 0.86 -4.6 565,198 - Pf6N2E2_497 0.86 -3.0 565,256 + -3.1 565,262 + -2.4 565,262 + -2.1 565,656 + -4.3 565,658 + -3.9 565,658 + -3.8 565,658 + -4.8 565,658 + -1.3 565,684 + -4.1 565,684 + -3.0 566,224 + Pf6N2E2_498 0.45 -0.1 566,224 + Pf6N2E2_498 0.45 -3.1 566,234 + Pf6N2E2_498 0.46 -2.2 566,262 - Pf6N2E2_498 0.48 -2.3 566,306 + Pf6N2E2_498 0.51 -4.5 566,549 + Pf6N2E2_498 0.68 -2.0 566,549 + Pf6N2E2_498 0.68 -3.8 566,829 + Pf6N2E2_498 0.87 -2.7 566,829 + Pf6N2E2_498 0.87 -3.8 566,829 + Pf6N2E2_498 0.87 -3.6 566,830 - Pf6N2E2_498 0.87 -3.2 566,830 - Pf6N2E2_498 0.87 -2.3 566,864 + Pf6N2E2_498 0.90 -2.5 566,864 + Pf6N2E2_498 0.90 -1.8 566,864 + Pf6N2E2_498 0.90 -2.5 566,865 - Pf6N2E2_498 0.90 -2.9 566,979 + -4.1 567,061 + -3.6 567,062 - -3.6 567,077 + -2.8 567,095 + -1.3 567,096 - -2.7 567,181 - -0.1 567,288 + -3.7 567,392 - -3.5 567,406 + -2.8 567,473 + -3.4 567,473 + -3.4 567,475 + -1.9 567,567 + -3.2 567,567 + -2.7 567,701 + -2.1 567,701 + -1.2 567,701 + -2.5 567,702 - -2.3 567,798 + Pf6N2E2_499 0.12 -3.3 567,798 + Pf6N2E2_499 0.12 -2.7 567,799 - Pf6N2E2_499 0.12 -3.9 567,799 - Pf6N2E2_499 0.12 -2.3
Or see this region's nucleotide sequence