Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5298 and Pf6N2E2_5299 overlap by 4 nucleotides Pf6N2E2_5299 and Pf6N2E2_5300 are separated by 342 nucleotides
Pf6N2E2_5298 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 6,091,686 to 6,092,858
_5298
Pf6N2E2_5299 - Benzoate transport protein, at 6,092,855 to 6,094,060
_5299
Pf6N2E2_5300 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 6,094,403 to 6,095,794
_5300
Position (kb)
6092
6093
6094
6095 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6091.886 kb on + strand, within Pf6N2E2_5298 at 6091.886 kb on + strand, within Pf6N2E2_5298 at 6092.140 kb on + strand, within Pf6N2E2_5298 at 6092.141 kb on - strand, within Pf6N2E2_5298 at 6092.181 kb on + strand, within Pf6N2E2_5298 at 6092.181 kb on + strand, within Pf6N2E2_5298 at 6092.395 kb on + strand, within Pf6N2E2_5298 at 6092.443 kb on + strand, within Pf6N2E2_5298 at 6092.443 kb on + strand, within Pf6N2E2_5298 at 6092.477 kb on - strand, within Pf6N2E2_5298 at 6092.613 kb on + strand, within Pf6N2E2_5298 at 6092.614 kb on - strand, within Pf6N2E2_5298 at 6092.614 kb on - strand, within Pf6N2E2_5298 at 6092.698 kb on + strand, within Pf6N2E2_5298 at 6092.741 kb on - strand at 6092.741 kb on - strand at 6092.797 kb on + strand at 6092.904 kb on + strand at 6092.904 kb on + strand at 6092.904 kb on + strand at 6092.905 kb on - strand at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.162 kb on + strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.163 kb on - strand, within Pf6N2E2_5299 at 6093.192 kb on + strand, within Pf6N2E2_5299 at 6093.192 kb on + strand, within Pf6N2E2_5299 at 6093.193 kb on - strand, within Pf6N2E2_5299 at 6093.193 kb on - strand, within Pf6N2E2_5299 at 6093.356 kb on + strand, within Pf6N2E2_5299 at 6093.375 kb on + strand, within Pf6N2E2_5299 at 6093.375 kb on + strand, within Pf6N2E2_5299 at 6093.376 kb on - strand, within Pf6N2E2_5299 at 6093.376 kb on - strand, within Pf6N2E2_5299 at 6093.631 kb on + strand, within Pf6N2E2_5299 at 6093.632 kb on - strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.765 kb on + strand, within Pf6N2E2_5299 at 6093.766 kb on - strand at 6093.766 kb on - strand, within Pf6N2E2_5299 at 6093.766 kb on - strand, within Pf6N2E2_5299 at 6093.815 kb on + strand, within Pf6N2E2_5299 at 6093.976 kb on + strand at 6093.976 kb on + strand at 6093.976 kb on + strand at 6093.976 kb on + strand at 6093.976 kb on + strand at 6093.977 kb on - strand at 6093.977 kb on - strand at 6093.977 kb on - strand at 6093.977 kb on - strand at 6093.977 kb on - strand at 6093.977 kb on - strand at 6093.977 kb on - strand at 6094.037 kb on + strand at 6094.037 kb on + strand at 6094.037 kb on + strand at 6094.037 kb on + strand at 6094.037 kb on + strand at 6094.037 kb on - strand at 6094.038 kb on - strand at 6094.038 kb on - strand at 6094.038 kb on - strand at 6094.038 kb on - strand at 6094.038 kb on - strand at 6094.419 kb on + strand at 6094.442 kb on + strand at 6094.442 kb on + strand at 6094.443 kb on - strand at 6094.443 kb on - strand at 6094.443 kb on - strand at 6094.443 kb on - strand at 6094.443 kb on - strand at 6094.571 kb on + strand, within Pf6N2E2_5300 at 6094.571 kb on + strand, within Pf6N2E2_5300 at 6094.572 kb on - strand, within Pf6N2E2_5300 at 6094.573 kb on + strand, within Pf6N2E2_5300 at 6094.625 kb on + strand, within Pf6N2E2_5300 at 6094.625 kb on + strand, within Pf6N2E2_5300 at 6094.625 kb on + strand, within Pf6N2E2_5300 at 6094.625 kb on + strand, within Pf6N2E2_5300 at 6094.730 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.968 kb on + strand, within Pf6N2E2_5300 at 6094.969 kb on - strand, within Pf6N2E2_5300
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 6,091,886 + Pf6N2E2_5298 0.17 +0.7 6,091,886 + Pf6N2E2_5298 0.17 +1.5 6,092,140 + Pf6N2E2_5298 0.39 -0.2 6,092,141 - Pf6N2E2_5298 0.39 +0.1 6,092,181 + Pf6N2E2_5298 0.42 -0.9 6,092,181 + Pf6N2E2_5298 0.42 +0.7 6,092,395 + Pf6N2E2_5298 0.60 -0.3 6,092,443 + Pf6N2E2_5298 0.65 +0.0 6,092,443 + Pf6N2E2_5298 0.65 -1.4 6,092,477 - Pf6N2E2_5298 0.67 +0.1 6,092,613 + Pf6N2E2_5298 0.79 -0.8 6,092,614 - Pf6N2E2_5298 0.79 -1.6 6,092,614 - Pf6N2E2_5298 0.79 -0.9 6,092,698 + Pf6N2E2_5298 0.86 +0.5 6,092,741 - -0.5 6,092,741 - +0.8 6,092,797 + -0.4 6,092,904 + -2.0 6,092,904 + -0.7 6,092,904 + -2.1 6,092,905 - -0.6 6,093,162 + Pf6N2E2_5299 0.25 -2.7 6,093,162 + Pf6N2E2_5299 0.25 -2.3 6,093,162 + Pf6N2E2_5299 0.25 -2.2 6,093,162 + Pf6N2E2_5299 0.25 -0.4 6,093,162 + Pf6N2E2_5299 0.25 -4.4 6,093,162 + Pf6N2E2_5299 0.25 -3.5 6,093,163 - Pf6N2E2_5299 0.26 -0.7 6,093,163 - Pf6N2E2_5299 0.26 -2.1 6,093,163 - Pf6N2E2_5299 0.26 -4.1 6,093,163 - Pf6N2E2_5299 0.26 -1.3 6,093,192 + Pf6N2E2_5299 0.28 -3.3 6,093,192 + Pf6N2E2_5299 0.28 -1.7 6,093,193 - Pf6N2E2_5299 0.28 -0.1 6,093,193 - Pf6N2E2_5299 0.28 -2.9 6,093,356 + Pf6N2E2_5299 0.42 -1.1 6,093,375 + Pf6N2E2_5299 0.43 -1.6 6,093,375 + Pf6N2E2_5299 0.43 -2.8 6,093,376 - Pf6N2E2_5299 0.43 -2.2 6,093,376 - Pf6N2E2_5299 0.43 +0.2 6,093,631 + Pf6N2E2_5299 0.64 -2.1 6,093,632 - Pf6N2E2_5299 0.64 -1.1 6,093,765 + Pf6N2E2_5299 0.75 -0.8 6,093,765 + Pf6N2E2_5299 0.75 -3.3 6,093,765 + Pf6N2E2_5299 0.75 -3.0 6,093,765 + Pf6N2E2_5299 0.75 -1.3 6,093,765 + Pf6N2E2_5299 0.75 -3.7 6,093,765 + Pf6N2E2_5299 0.75 -2.8 6,093,765 + Pf6N2E2_5299 0.75 -1.8 6,093,766 - +0.3 6,093,766 - Pf6N2E2_5299 0.76 -2.8 6,093,766 - Pf6N2E2_5299 0.76 -1.7 6,093,815 + Pf6N2E2_5299 0.80 -0.7 6,093,976 + -1.8 6,093,976 + -1.3 6,093,976 + -1.8 6,093,976 + -0.9 6,093,976 + +0.2 6,093,977 - -1.4 6,093,977 - -3.3 6,093,977 - -2.8 6,093,977 - -3.9 6,093,977 - +0.2 6,093,977 - -1.4 6,093,977 - -0.3 6,094,037 + -2.8 6,094,037 + -2.3 6,094,037 + -2.8 6,094,037 + -1.8 6,094,037 + +0.3 6,094,037 - -2.3 6,094,038 - -3.3 6,094,038 - -2.7 6,094,038 - -2.3 6,094,038 - -2.5 6,094,038 - -0.5 6,094,419 + +0.6 6,094,442 + -0.4 6,094,442 + +0.4 6,094,443 - +1.9 6,094,443 - -0.4 6,094,443 - +0.3 6,094,443 - +1.2 6,094,443 - -0.1 6,094,571 + Pf6N2E2_5300 0.12 +0.1 6,094,571 + Pf6N2E2_5300 0.12 +0.6 6,094,572 - Pf6N2E2_5300 0.12 +0.6 6,094,573 + Pf6N2E2_5300 0.12 +0.9 6,094,625 + Pf6N2E2_5300 0.16 +0.1 6,094,625 + Pf6N2E2_5300 0.16 -3.6 6,094,625 + Pf6N2E2_5300 0.16 -0.6 6,094,625 + Pf6N2E2_5300 0.16 +0.3 6,094,730 + Pf6N2E2_5300 0.23 +0.7 6,094,968 + Pf6N2E2_5300 0.41 +0.3 6,094,968 + Pf6N2E2_5300 0.41 -1.3 6,094,968 + Pf6N2E2_5300 0.41 -0.1 6,094,968 + Pf6N2E2_5300 0.41 +0.4 6,094,968 + Pf6N2E2_5300 0.41 +1.7 6,094,968 + Pf6N2E2_5300 0.41 -0.4 6,094,968 + Pf6N2E2_5300 0.41 +0.6 6,094,969 - Pf6N2E2_5300 0.41 -1.4
Or see this region's nucleotide sequence