Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3571 and Pf6N2E2_3572 are separated by 9 nucleotides Pf6N2E2_3572 and Pf6N2E2_3573 are separated by 31 nucleotides Pf6N2E2_3573 and Pf6N2E2_3574 are separated by 184 nucleotides
Pf6N2E2_3571 - Urease accessory protein UreF, at 4,162,116 to 4,162,790
_3571
Pf6N2E2_3572 - Urease accessory protein UreG, at 4,162,800 to 4,163,414
_3572
Pf6N2E2_3573 - HupE-UreJ family metal transporter, at 4,163,446 to 4,164,018
_3573
Pf6N2E2_3574 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 4,164,203 to 4,165,336
_3574
Position (kb)
4163
4164
4165 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 4162.540 kb on + strand, within Pf6N2E2_3571 at 4162.540 kb on + strand, within Pf6N2E2_3571 at 4162.541 kb on - strand, within Pf6N2E2_3571 at 4162.541 kb on - strand, within Pf6N2E2_3571 at 4162.767 kb on + strand at 4162.767 kb on + strand at 4162.767 kb on + strand at 4162.788 kb on + strand at 4162.789 kb on - strand at 4162.805 kb on + strand at 4162.805 kb on + strand at 4162.805 kb on + strand at 4162.805 kb on + strand at 4162.806 kb on - strand at 4162.806 kb on - strand at 4162.806 kb on - strand at 4162.806 kb on - strand at 4162.840 kb on + strand at 4162.840 kb on + strand at 4162.841 kb on - strand at 4162.885 kb on + strand, within Pf6N2E2_3572 at 4162.886 kb on - strand, within Pf6N2E2_3572 at 4162.897 kb on - strand, within Pf6N2E2_3572 at 4162.897 kb on - strand at 4162.897 kb on - strand, within Pf6N2E2_3572 at 4162.926 kb on + strand, within Pf6N2E2_3572 at 4162.926 kb on + strand, within Pf6N2E2_3572 at 4162.926 kb on + strand, within Pf6N2E2_3572 at 4162.926 kb on + strand, within Pf6N2E2_3572 at 4162.926 kb on + strand, within Pf6N2E2_3572 at 4162.926 kb on + strand, within Pf6N2E2_3572 at 4162.927 kb on - strand, within Pf6N2E2_3572 at 4162.927 kb on - strand, within Pf6N2E2_3572 at 4162.927 kb on - strand, within Pf6N2E2_3572 at 4162.927 kb on - strand, within Pf6N2E2_3572 at 4162.927 kb on - strand, within Pf6N2E2_3572 at 4162.927 kb on - strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.163 kb on + strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.164 kb on - strand, within Pf6N2E2_3572 at 4163.246 kb on + strand, within Pf6N2E2_3572 at 4163.247 kb on - strand, within Pf6N2E2_3572 at 4163.333 kb on - strand, within Pf6N2E2_3572 at 4163.441 kb on + strand at 4163.658 kb on + strand, within Pf6N2E2_3573 at 4163.659 kb on - strand, within Pf6N2E2_3573 at 4163.659 kb on - strand, within Pf6N2E2_3573 at 4163.659 kb on - strand, within Pf6N2E2_3573 at 4163.872 kb on + strand, within Pf6N2E2_3573 at 4164.089 kb on + strand at 4164.089 kb on + strand at 4164.089 kb on + strand at 4164.090 kb on - strand at 4164.090 kb on - strand at 4164.090 kb on - strand at 4164.090 kb on - strand at 4164.160 kb on + strand at 4164.161 kb on - strand at 4164.161 kb on - strand at 4164.161 kb on - strand at 4164.161 kb on - strand at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.410 kb on + strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.411 kb on - strand, within Pf6N2E2_3574 at 4164.521 kb on + strand, within Pf6N2E2_3574 at 4164.521 kb on + strand, within Pf6N2E2_3574 at 4164.521 kb on + strand, within Pf6N2E2_3574 at 4164.522 kb on - strand, within Pf6N2E2_3574 at 4164.569 kb on + strand, within Pf6N2E2_3574 at 4164.569 kb on + strand, within Pf6N2E2_3574 at 4164.570 kb on - strand, within Pf6N2E2_3574 at 4164.570 kb on - strand, within Pf6N2E2_3574 at 4164.570 kb on - strand, within Pf6N2E2_3574 at 4164.570 kb on - strand, within Pf6N2E2_3574 at 4164.570 kb on - strand, within Pf6N2E2_3574 at 4164.583 kb on + strand, within Pf6N2E2_3574 at 4164.698 kb on + strand, within Pf6N2E2_3574 at 4164.699 kb on - strand, within Pf6N2E2_3574 at 4164.712 kb on - strand, within Pf6N2E2_3574 at 4164.913 kb on + strand, within Pf6N2E2_3574 at 4164.947 kb on + strand, within Pf6N2E2_3574 at 4164.948 kb on - strand, within Pf6N2E2_3574
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 4,162,540 + Pf6N2E2_3571 0.63 -4.2 4,162,540 + Pf6N2E2_3571 0.63 -1.2 4,162,541 - Pf6N2E2_3571 0.63 -1.8 4,162,541 - Pf6N2E2_3571 0.63 -2.0 4,162,767 + -3.0 4,162,767 + +0.5 4,162,767 + -2.6 4,162,788 + +7.0 4,162,789 - -3.3 4,162,805 + -3.1 4,162,805 + -2.1 4,162,805 + -1.9 4,162,805 + -2.2 4,162,806 - -1.5 4,162,806 - -3.9 4,162,806 - -1.8 4,162,806 - -2.3 4,162,840 + -1.5 4,162,840 + -1.1 4,162,841 - -3.5 4,162,885 + Pf6N2E2_3572 0.14 -1.3 4,162,886 - Pf6N2E2_3572 0.14 -2.2 4,162,897 - Pf6N2E2_3572 0.16 -3.5 4,162,897 - +1.5 4,162,897 - Pf6N2E2_3572 0.16 -1.4 4,162,926 + Pf6N2E2_3572 0.20 -2.9 4,162,926 + Pf6N2E2_3572 0.20 -1.5 4,162,926 + Pf6N2E2_3572 0.20 -3.3 4,162,926 + Pf6N2E2_3572 0.20 -1.6 4,162,926 + Pf6N2E2_3572 0.20 -2.5 4,162,926 + Pf6N2E2_3572 0.20 -1.7 4,162,927 - Pf6N2E2_3572 0.21 -4.2 4,162,927 - Pf6N2E2_3572 0.21 -1.6 4,162,927 - Pf6N2E2_3572 0.21 -3.1 4,162,927 - Pf6N2E2_3572 0.21 -0.5 4,162,927 - Pf6N2E2_3572 0.21 -3.3 4,162,927 - Pf6N2E2_3572 0.21 -0.5 4,163,163 + Pf6N2E2_3572 0.59 -3.3 4,163,163 + Pf6N2E2_3572 0.59 -1.1 4,163,163 + Pf6N2E2_3572 0.59 -2.6 4,163,163 + Pf6N2E2_3572 0.59 -2.8 4,163,163 + Pf6N2E2_3572 0.59 -2.2 4,163,163 + Pf6N2E2_3572 0.59 -3.3 4,163,163 + Pf6N2E2_3572 0.59 -2.9 4,163,164 - Pf6N2E2_3572 0.59 -3.4 4,163,164 - Pf6N2E2_3572 0.59 -1.9 4,163,164 - Pf6N2E2_3572 0.59 -2.3 4,163,164 - Pf6N2E2_3572 0.59 -3.3 4,163,164 - Pf6N2E2_3572 0.59 -1.6 4,163,164 - Pf6N2E2_3572 0.59 -2.9 4,163,164 - Pf6N2E2_3572 0.59 -1.3 4,163,164 - Pf6N2E2_3572 0.59 -1.7 4,163,164 - Pf6N2E2_3572 0.59 -2.7 4,163,164 - Pf6N2E2_3572 0.59 -2.1 4,163,164 - Pf6N2E2_3572 0.59 -2.4 4,163,164 - Pf6N2E2_3572 0.59 -2.8 4,163,164 - Pf6N2E2_3572 0.59 -0.4 4,163,164 - Pf6N2E2_3572 0.59 -1.2 4,163,164 - Pf6N2E2_3572 0.59 -1.2 4,163,164 - Pf6N2E2_3572 0.59 -2.0 4,163,164 - Pf6N2E2_3572 0.59 -1.6 4,163,164 - Pf6N2E2_3572 0.59 -0.6 4,163,246 + Pf6N2E2_3572 0.73 -1.6 4,163,247 - Pf6N2E2_3572 0.73 -2.0 4,163,333 - Pf6N2E2_3572 0.87 -3.9 4,163,441 + -3.3 4,163,658 + Pf6N2E2_3573 0.37 -3.7 4,163,659 - Pf6N2E2_3573 0.37 -2.6 4,163,659 - Pf6N2E2_3573 0.37 -1.1 4,163,659 - Pf6N2E2_3573 0.37 -0.4 4,163,872 + Pf6N2E2_3573 0.74 -3.2 4,164,089 + +0.1 4,164,089 + -3.2 4,164,089 + -1.4 4,164,090 - -2.9 4,164,090 - -1.2 4,164,090 - -2.5 4,164,090 - -3.1 4,164,160 + -0.5 4,164,161 - -0.7 4,164,161 - -0.0 4,164,161 - -2.1 4,164,161 - +0.7 4,164,410 + Pf6N2E2_3574 0.18 +0.3 4,164,410 + Pf6N2E2_3574 0.18 -0.5 4,164,410 + Pf6N2E2_3574 0.18 -0.3 4,164,410 + Pf6N2E2_3574 0.18 -0.5 4,164,410 + Pf6N2E2_3574 0.18 -1.1 4,164,410 + Pf6N2E2_3574 0.18 +0.5 4,164,410 + Pf6N2E2_3574 0.18 +0.8 4,164,410 + Pf6N2E2_3574 0.18 -1.0 4,164,410 + Pf6N2E2_3574 0.18 -1.2 4,164,410 + Pf6N2E2_3574 0.18 -1.0 4,164,410 + Pf6N2E2_3574 0.18 +0.1 4,164,410 + Pf6N2E2_3574 0.18 -0.3 4,164,410 + Pf6N2E2_3574 0.18 -0.8 4,164,410 + Pf6N2E2_3574 0.18 -0.1 4,164,410 + Pf6N2E2_3574 0.18 +0.2 4,164,410 + Pf6N2E2_3574 0.18 -0.9 4,164,410 + Pf6N2E2_3574 0.18 +0.2 4,164,410 + Pf6N2E2_3574 0.18 +0.7 4,164,410 + Pf6N2E2_3574 0.18 +1.1 4,164,411 - Pf6N2E2_3574 0.18 -0.1 4,164,411 - Pf6N2E2_3574 0.18 -0.1 4,164,411 - Pf6N2E2_3574 0.18 -1.1 4,164,411 - Pf6N2E2_3574 0.18 -1.4 4,164,411 - Pf6N2E2_3574 0.18 -0.7 4,164,411 - Pf6N2E2_3574 0.18 -0.8 4,164,411 - Pf6N2E2_3574 0.18 -0.3 4,164,411 - Pf6N2E2_3574 0.18 +0.2 4,164,411 - Pf6N2E2_3574 0.18 -0.1 4,164,411 - Pf6N2E2_3574 0.18 -0.2 4,164,411 - Pf6N2E2_3574 0.18 +0.1 4,164,411 - Pf6N2E2_3574 0.18 -0.2 4,164,411 - Pf6N2E2_3574 0.18 -1.2 4,164,411 - Pf6N2E2_3574 0.18 +0.6 4,164,411 - Pf6N2E2_3574 0.18 -0.5 4,164,411 - Pf6N2E2_3574 0.18 +0.1 4,164,411 - Pf6N2E2_3574 0.18 +0.2 4,164,411 - Pf6N2E2_3574 0.18 -0.7 4,164,521 + Pf6N2E2_3574 0.28 +0.3 4,164,521 + Pf6N2E2_3574 0.28 +0.4 4,164,521 + Pf6N2E2_3574 0.28 +0.5 4,164,522 - Pf6N2E2_3574 0.28 +0.2 4,164,569 + Pf6N2E2_3574 0.32 -0.3 4,164,569 + Pf6N2E2_3574 0.32 -0.1 4,164,570 - Pf6N2E2_3574 0.32 -1.4 4,164,570 - Pf6N2E2_3574 0.32 -0.6 4,164,570 - Pf6N2E2_3574 0.32 -0.6 4,164,570 - Pf6N2E2_3574 0.32 +0.1 4,164,570 - Pf6N2E2_3574 0.32 +0.7 4,164,583 + Pf6N2E2_3574 0.34 +0.8 4,164,698 + Pf6N2E2_3574 0.44 +0.1 4,164,699 - Pf6N2E2_3574 0.44 -0.7 4,164,712 - Pf6N2E2_3574 0.45 -0.9 4,164,913 + Pf6N2E2_3574 0.63 +0.7 4,164,947 + Pf6N2E2_3574 0.66 -1.4 4,164,948 - Pf6N2E2_3574 0.66 -0.7
Or see this region's nucleotide sequence