Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1681 and Pf6N2E2_1682 are separated by 84 nucleotides Pf6N2E2_1682 and Pf6N2E2_1683 are separated by 0 nucleotides Pf6N2E2_1683 and Pf6N2E2_1684 are separated by 147 nucleotides
Pf6N2E2_1681 - Multidrug resistance protein B, at 1,988,915 to 1,990,141
_1681
Pf6N2E2_1682 - RNA polymerase sigma-70 factor, ECF subfamily, at 1,990,226 to 1,990,741
_1682
Pf6N2E2_1683 - Fe2+-dicitrate sensor, membrane component, at 1,990,742 to 1,991,719
_1683
Pf6N2E2_1684 - Ferrichrome-iron receptor, at 1,991,867 to 1,994,230
_1684
Position (kb)
1990
1991
1992 Strain fitness (log2 ratio)
-2
-1
0
1 at 1989.794 kb on + strand, within Pf6N2E2_1681 at 1989.794 kb on + strand, within Pf6N2E2_1681 at 1989.795 kb on - strand, within Pf6N2E2_1681 at 1989.855 kb on + strand, within Pf6N2E2_1681 at 1989.856 kb on - strand, within Pf6N2E2_1681 at 1989.856 kb on - strand, within Pf6N2E2_1681 at 1990.049 kb on + strand at 1990.050 kb on - strand at 1990.050 kb on - strand at 1990.050 kb on - strand at 1990.055 kb on + strand at 1990.055 kb on + strand at 1990.055 kb on + strand at 1990.056 kb on - strand at 1990.056 kb on - strand at 1990.076 kb on + strand at 1990.077 kb on - strand at 1990.115 kb on - strand at 1990.283 kb on + strand, within Pf6N2E2_1682 at 1990.421 kb on + strand, within Pf6N2E2_1682 at 1990.422 kb on - strand, within Pf6N2E2_1682 at 1990.422 kb on - strand, within Pf6N2E2_1682 at 1990.646 kb on + strand, within Pf6N2E2_1682 at 1990.697 kb on + strand at 1990.698 kb on - strand at 1991.170 kb on - strand, within Pf6N2E2_1683 at 1991.349 kb on + strand, within Pf6N2E2_1683 at 1991.733 kb on - strand at 1992.090 kb on - strand at 1992.118 kb on - strand, within Pf6N2E2_1684 at 1992.165 kb on - strand, within Pf6N2E2_1684 at 1992.244 kb on + strand, within Pf6N2E2_1684 at 1992.335 kb on + strand, within Pf6N2E2_1684 at 1992.335 kb on + strand, within Pf6N2E2_1684 at 1992.336 kb on - strand, within Pf6N2E2_1684 at 1992.336 kb on - strand, within Pf6N2E2_1684 at 1992.507 kb on - strand, within Pf6N2E2_1684 at 1992.507 kb on - strand, within Pf6N2E2_1684 at 1992.543 kb on - strand, within Pf6N2E2_1684 at 1992.588 kb on - strand, within Pf6N2E2_1684
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 1,989,794 + Pf6N2E2_1681 0.72 +0.1 1,989,794 + Pf6N2E2_1681 0.72 +1.7 1,989,795 - Pf6N2E2_1681 0.72 +1.1 1,989,855 + Pf6N2E2_1681 0.77 +1.1 1,989,856 - Pf6N2E2_1681 0.77 +0.8 1,989,856 - Pf6N2E2_1681 0.77 -2.0 1,990,049 + +0.2 1,990,050 - +0.6 1,990,050 - +0.2 1,990,050 - -0.6 1,990,055 + -1.0 1,990,055 + +0.5 1,990,055 + -0.0 1,990,056 - +0.1 1,990,056 - -0.1 1,990,076 + -1.1 1,990,077 - -0.7 1,990,115 - -0.1 1,990,283 + Pf6N2E2_1682 0.11 +0.3 1,990,421 + Pf6N2E2_1682 0.38 -0.8 1,990,422 - Pf6N2E2_1682 0.38 -0.7 1,990,422 - Pf6N2E2_1682 0.38 +0.3 1,990,646 + Pf6N2E2_1682 0.81 +0.4 1,990,697 + +0.4 1,990,698 - -0.1 1,991,170 - Pf6N2E2_1683 0.44 -0.7 1,991,349 + Pf6N2E2_1683 0.62 -1.8 1,991,733 - -0.6 1,992,090 - -0.2 1,992,118 - Pf6N2E2_1684 0.11 -0.7 1,992,165 - Pf6N2E2_1684 0.13 +1.1 1,992,244 + Pf6N2E2_1684 0.16 +0.1 1,992,335 + Pf6N2E2_1684 0.20 +0.3 1,992,335 + Pf6N2E2_1684 0.20 -0.1 1,992,336 - Pf6N2E2_1684 0.20 -0.8 1,992,336 - Pf6N2E2_1684 0.20 -0.2 1,992,507 - Pf6N2E2_1684 0.27 -0.5 1,992,507 - Pf6N2E2_1684 0.27 +0.2 1,992,543 - Pf6N2E2_1684 0.29 +0.2 1,992,588 - Pf6N2E2_1684 0.30 +1.6
Or see this region's nucleotide sequence