Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1622 and Pf6N2E2_1623 are separated by 157 nucleotides Pf6N2E2_1623 and Pf6N2E2_1624 are separated by 36 nucleotides Pf6N2E2_1624 and Pf6N2E2_1625 are separated by 203 nucleotides
Pf6N2E2_1622 - Methyl-accepting chemotaxis protein, at 1,896,899 to 1,898,869
_1622
Pf6N2E2_1623 - Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76), at 1,899,027 to 1,900,457
_1623
Pf6N2E2_1624 - MbtH-like protein, at 1,900,494 to 1,900,709
_1624
Pf6N2E2_1625 - Ferric reductase (1.6.99.14), at 1,900,913 to 1,901,650
_1625
Position (kb)
1899
1900
1901 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1898.087 kb on + strand, within Pf6N2E2_1622 at 1898.088 kb on - strand, within Pf6N2E2_1622 at 1898.111 kb on + strand, within Pf6N2E2_1622 at 1898.112 kb on - strand, within Pf6N2E2_1622 at 1898.292 kb on + strand, within Pf6N2E2_1622 at 1898.292 kb on + strand, within Pf6N2E2_1622 at 1898.293 kb on - strand, within Pf6N2E2_1622 at 1898.345 kb on + strand, within Pf6N2E2_1622 at 1898.358 kb on + strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.359 kb on - strand, within Pf6N2E2_1622 at 1898.518 kb on - strand, within Pf6N2E2_1622 at 1899.036 kb on - strand at 1899.307 kb on - strand, within Pf6N2E2_1623 at 1899.489 kb on - strand, within Pf6N2E2_1623 at 1899.552 kb on + strand, within Pf6N2E2_1623 at 1899.553 kb on - strand, within Pf6N2E2_1623 at 1899.691 kb on - strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.276 kb on + strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.277 kb on - strand, within Pf6N2E2_1623 at 1900.434 kb on + strand at 1900.434 kb on + strand at 1900.435 kb on - strand at 1900.435 kb on - strand at 1900.473 kb on + strand at 1900.509 kb on + strand at 1900.509 kb on + strand at 1900.510 kb on - strand at 1900.510 kb on - strand at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.567 kb on + strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.568 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.672 kb on - strand, within Pf6N2E2_1624 at 1900.751 kb on - strand at 1900.855 kb on - strand at 1900.855 kb on - strand at 1900.856 kb on + strand at 1900.856 kb on + strand at 1900.857 kb on - strand at 1900.857 kb on - strand at 1901.001 kb on + strand, within Pf6N2E2_1625 at 1901.031 kb on - strand, within Pf6N2E2_1625 at 1901.291 kb on - strand, within Pf6N2E2_1625 at 1901.383 kb on + strand, within Pf6N2E2_1625 at 1901.384 kb on - strand, within Pf6N2E2_1625
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 1,898,087 + Pf6N2E2_1622 0.60 +1.7 1,898,088 - Pf6N2E2_1622 0.60 +0.8 1,898,111 + Pf6N2E2_1622 0.61 +2.2 1,898,112 - Pf6N2E2_1622 0.62 -1.0 1,898,292 + Pf6N2E2_1622 0.71 -2.1 1,898,292 + Pf6N2E2_1622 0.71 -0.9 1,898,293 - Pf6N2E2_1622 0.71 -0.8 1,898,345 + Pf6N2E2_1622 0.73 -0.8 1,898,358 + Pf6N2E2_1622 0.74 -0.7 1,898,359 - Pf6N2E2_1622 0.74 -1.1 1,898,359 - Pf6N2E2_1622 0.74 -0.2 1,898,359 - Pf6N2E2_1622 0.74 -0.5 1,898,518 - Pf6N2E2_1622 0.82 +0.4 1,899,036 - +0.1 1,899,307 - Pf6N2E2_1623 0.20 -0.5 1,899,489 - Pf6N2E2_1623 0.32 -2.0 1,899,552 + Pf6N2E2_1623 0.37 -0.5 1,899,553 - Pf6N2E2_1623 0.37 -0.2 1,899,691 - Pf6N2E2_1623 0.46 -0.7 1,900,276 + Pf6N2E2_1623 0.87 +0.1 1,900,276 + Pf6N2E2_1623 0.87 -1.7 1,900,277 - Pf6N2E2_1623 0.87 -0.3 1,900,277 - Pf6N2E2_1623 0.87 -0.3 1,900,434 + -0.3 1,900,434 + -0.5 1,900,435 - -0.7 1,900,435 - -0.1 1,900,473 + -0.4 1,900,509 + +0.2 1,900,509 + -0.2 1,900,510 - +0.7 1,900,510 - +1.4 1,900,567 + Pf6N2E2_1624 0.34 -0.1 1,900,567 + Pf6N2E2_1624 0.34 +0.3 1,900,568 - Pf6N2E2_1624 0.34 -0.8 1,900,568 - Pf6N2E2_1624 0.34 -0.3 1,900,672 - Pf6N2E2_1624 0.82 -0.7 1,900,672 - Pf6N2E2_1624 0.82 +0.2 1,900,751 - -0.3 1,900,855 - +0.4 1,900,855 - +0.8 1,900,856 + -0.4 1,900,856 + -0.1 1,900,857 - +1.0 1,900,857 - -0.0 1,901,001 + Pf6N2E2_1625 0.12 -0.6 1,901,031 - Pf6N2E2_1625 0.16 -1.1 1,901,291 - Pf6N2E2_1625 0.51 +0.3 1,901,383 + Pf6N2E2_1625 0.64 -0.2 1,901,384 - Pf6N2E2_1625 0.64 +0.0
Or see this region's nucleotide sequence