Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23280

Experiment: Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23270 and BWI76_RS23275 are separated by 12 nucleotidesBWI76_RS23275 and BWI76_RS23280 are separated by 39 nucleotidesBWI76_RS23280 and BWI76_RS23285 are separated by 219 nucleotidesBWI76_RS23285 and BWI76_RS23290 are separated by 16 nucleotides BWI76_RS23270: BWI76_RS23270 - phosphoenolpyruvate--protein phosphotransferase PtsP, at 4,854,084 to 4,856,330 _RS23270 BWI76_RS23275: BWI76_RS23275 - RNA pyrophosphohydrolase, at 4,856,343 to 4,856,873 _RS23275 BWI76_RS23280: BWI76_RS23280 - hypothetical protein, at 4,856,913 to 4,857,095 _RS23280 BWI76_RS23285: BWI76_RS23285 - hypothetical protein, at 4,857,315 to 4,857,539 _RS23285 BWI76_RS23290: BWI76_RS23290 - DNA mismatch repair protein MutH, at 4,857,556 to 4,858,248 _RS23290 Position (kb) 4856 4857 4858Strain fitness (log2 ratio) -1 0 1at 4855.935 kb on - strand, within BWI76_RS23270at 4856.079 kb on - strand, within BWI76_RS23270at 4856.334 kb on - strandat 4856.389 kb on - strandat 4856.929 kb on + strandat 4856.995 kb on + strand, within BWI76_RS23280at 4857.274 kb on - strandat 4857.369 kb on + strand, within BWI76_RS23285at 4857.584 kb on - strandat 4857.656 kb on + strand, within BWI76_RS23290at 4857.840 kb on - strand, within BWI76_RS23290at 4857.853 kb on + strand, within BWI76_RS23290at 4857.927 kb on + strand, within BWI76_RS23290at 4857.956 kb on - strand, within BWI76_RS23290at 4858.052 kb on + strand, within BWI76_RS23290at 4858.060 kb on - strand, within BWI76_RS23290

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
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4,855,935 - BWI76_RS23270 0.82 -0.8
4,856,079 - BWI76_RS23270 0.89 -1.4
4,856,334 - +0.5
4,856,389 - +1.2
4,856,929 + +0.1
4,856,995 + BWI76_RS23280 0.45 +0.1
4,857,274 - +0.3
4,857,369 + BWI76_RS23285 0.24 -0.5
4,857,584 - +0.0
4,857,656 + BWI76_RS23290 0.14 +0.4
4,857,840 - BWI76_RS23290 0.41 -0.8
4,857,853 + BWI76_RS23290 0.43 +0.2
4,857,927 + BWI76_RS23290 0.54 -0.6
4,857,956 - BWI76_RS23290 0.58 -0.7
4,858,052 + BWI76_RS23290 0.72 -0.1
4,858,060 - BWI76_RS23290 0.73 -0.6

Or see this region's nucleotide sequence