Experiment: MoLS4 minimal media with D,L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2438 and DvMF_2439 overlap by 4 nucleotides DvMF_2439 and DvMF_2440 are separated by 29 nucleotides DvMF_2440 and DvMF_2441 are separated by 14 nucleotides DvMF_2441 and DvMF_2442 are separated by 466 nucleotides
DvMF_2438: DvMF_2438 - type III secretion system apparatus protein YscQ/HrcQ (RefSeq), at 3,072,806 to 3,074,035
_2438
DvMF_2439: DvMF_2439 - type III secretion system protein (RefSeq), at 3,074,032 to 3,074,682
_2439
DvMF_2440: DvMF_2440 - anti-sigma-factor antagonist (RefSeq), at 3,074,712 to 3,075,047
_2440
DvMF_2441: DvMF_2441 - putative anti-sigma regulatory factor, serine/threonine protein kinase (RefSeq), at 3,075,062 to 3,075,478
_2441
DvMF_2442: DvMF_2442 - Lytic transglycosylase catalytic (RefSeq), at 3,075,945 to 3,076,682
_2442
Position (kb)
3074
3075
3076 Strain fitness (log2 ratio)
-1
0
1 at 3073.806 kb on + strand, within DvMF_2438 at 3073.999 kb on + strand at 3074.089 kb on - strand at 3074.484 kb on + strand, within DvMF_2439 at 3074.492 kb on - strand, within DvMF_2439 at 3074.624 kb on + strand at 3074.720 kb on + strand at 3074.720 kb on + strand at 3074.730 kb on + strand at 3074.865 kb on - strand, within DvMF_2440 at 3074.865 kb on - strand, within DvMF_2440 at 3074.911 kb on - strand, within DvMF_2440 at 3074.926 kb on + strand, within DvMF_2440 at 3075.539 kb on + strand at 3075.763 kb on + strand at 3075.881 kb on - strand at 3075.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Histidine remove 3,073,806 + DvMF_2438 0.81 -0.6 3,073,999 + -0.4 3,074,089 - -0.4 3,074,484 + DvMF_2439 0.69 -1.3 3,074,492 - DvMF_2439 0.71 -0.4 3,074,624 + -0.0 3,074,720 + -0.9 3,074,720 + -0.7 3,074,730 + -1.2 3,074,865 - DvMF_2440 0.46 -0.6 3,074,865 - DvMF_2440 0.46 -0.6 3,074,911 - DvMF_2440 0.59 -0.8 3,074,926 + DvMF_2440 0.64 -1.4 3,075,539 + -1.1 3,075,763 + -0.5 3,075,881 - -0.7 3,075,881 - -0.5
Or see this region's nucleotide sequence