Experiment: MoLS4 minimal media with D,L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2063 and DvMF_2064 are separated by 173 nucleotides DvMF_2064 and DvMF_2065 are separated by 227 nucleotides DvMF_2065 and DvMF_2066 are separated by 109 nucleotides
DvMF_2063: DvMF_2063 - protein of unknown function DUF214 (RefSeq), at 2,558,427 to 2,559,620
_2063
DvMF_2064: DvMF_2064 - hypothetical protein (RefSeq), at 2,559,794 to 2,560,255
_2064
DvMF_2065: DvMF_2065 - ybaK/ebsC protein (RefSeq), at 2,560,483 to 2,560,947
_2065
DvMF_2066: DvMF_2066 - heavy metal translocating P-type ATPase (RefSeq), at 2,561,057 to 2,563,573
_2066
Position (kb)
2559
2560
2561 Strain fitness (log2 ratio)
-1
0
1 at 2558.841 kb on - strand, within DvMF_2063 at 2558.866 kb on + strand, within DvMF_2063 at 2558.866 kb on + strand, within DvMF_2063 at 2558.874 kb on - strand, within DvMF_2063 at 2558.959 kb on - strand, within DvMF_2063 at 2559.210 kb on + strand, within DvMF_2063 at 2559.270 kb on - strand, within DvMF_2063 at 2559.614 kb on - strand at 2559.924 kb on + strand, within DvMF_2064 at 2559.983 kb on - strand, within DvMF_2064 at 2560.352 kb on - strand at 2560.401 kb on - strand at 2560.512 kb on + strand at 2560.608 kb on - strand, within DvMF_2065 at 2560.809 kb on + strand, within DvMF_2065 at 2560.898 kb on - strand, within DvMF_2065 at 2561.054 kb on + strand at 2561.142 kb on - strand at 2561.142 kb on - strand at 2561.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Histidine remove 2,558,841 - DvMF_2063 0.35 -1.3 2,558,866 + DvMF_2063 0.37 -0.9 2,558,866 + DvMF_2063 0.37 +0.7 2,558,874 - DvMF_2063 0.37 -0.4 2,558,959 - DvMF_2063 0.45 -0.6 2,559,210 + DvMF_2063 0.66 -0.7 2,559,270 - DvMF_2063 0.71 -0.8 2,559,614 - -0.5 2,559,924 + DvMF_2064 0.28 -0.3 2,559,983 - DvMF_2064 0.41 -1.6 2,560,352 - +0.6 2,560,401 - -0.7 2,560,512 + -0.1 2,560,608 - DvMF_2065 0.27 +0.2 2,560,809 + DvMF_2065 0.70 +0.1 2,560,898 - DvMF_2065 0.89 -0.8 2,561,054 + -0.9 2,561,142 - +1.5 2,561,142 - -0.2 2,561,172 - -0.5
Or see this region's nucleotide sequence