Experiment: MoLS4 minimal media with D,L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0350 and DvMF_0351 are separated by 306 nucleotides DvMF_0351 and DvMF_0352 are separated by 130 nucleotides DvMF_0352 and DvMF_0353 are separated by 285 nucleotides
DvMF_0350: DvMF_0350 - extracellular solute-binding protein family 3 (RefSeq), at 408,188 to 409,153
_0350
DvMF_0351: DvMF_0351 - hypothetical protein (RefSeq), at 409,460 to 409,624
_0351
DvMF_0352: DvMF_0352 - putative phage repressor (RefSeq), at 409,755 to 410,540
_0352
DvMF_0353: DvMF_0353 - diaminopimelate decarboxylase (RefSeq), at 410,826 to 412,076
_0353
Position (kb) 409
410
411 Strain fitness (log2 ratio) -1
0
1 at 408.795 kb on + strand, within DvMF_0350 at 408.837 kb on - strand, within DvMF_0350 at 408.913 kb on + strand, within DvMF_0350 at 408.924 kb on + strand, within DvMF_0350 at 408.934 kb on + strand, within DvMF_0350 at 409.141 kb on - strand at 409.181 kb on + strand at 409.244 kb on + strand at 409.244 kb on + strand at 409.303 kb on + strand at 409.321 kb on - strand at 409.486 kb on - strand, within DvMF_0351 at 409.500 kb on + strand, within DvMF_0351 at 409.575 kb on - strand, within DvMF_0351 at 409.577 kb on + strand, within DvMF_0351 at 409.577 kb on + strand, within DvMF_0351 at 409.584 kb on + strand, within DvMF_0351 at 410.550 kb on - strand at 410.876 kb on - strand at 411.149 kb on - strand, within DvMF_0353
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with D,L-Histidine remove 408,795 + DvMF_0350 0.63 -0.9 408,837 - DvMF_0350 0.67 -0.7 408,913 + DvMF_0350 0.75 -0.3 408,924 + DvMF_0350 0.76 -0.9 408,934 + DvMF_0350 0.77 -1.6 409,141 - -0.4 409,181 + +0.1 409,244 + -0.9 409,244 + -0.6 409,303 + -0.5 409,321 - -0.2 409,486 - DvMF_0351 0.16 -1.2 409,500 + DvMF_0351 0.24 -0.8 409,575 - DvMF_0351 0.70 -0.4 409,577 + DvMF_0351 0.71 -0.8 409,577 + DvMF_0351 0.71 -0.7 409,584 + DvMF_0351 0.75 -1.1 410,550 - -0.4 410,876 - -0.1 411,149 - DvMF_0353 0.26 -0.9
Or see this region's nucleotide sequence