Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3054

Experiment: Putrescine (N)

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPf6N2E2_3053 and Pf6N2E2_3054 are separated by 149 nucleotidesPf6N2E2_3054 and Pf6N2E2_3055 are separated by 194 nucleotidesPf6N2E2_3055 and Pf6N2E2_3056 are separated by 17 nucleotides Pf6N2E2_3053 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 3,536,772 to 3,537,719 _3053 Pf6N2E2_3054 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,537,869 to 3,541,390 _3054 Pf6N2E2_3055 - Ribonuclease HII (EC 3.1.26.4), at 3,541,585 to 3,542,217 _3055 Pf6N2E2_3056 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 3,542,235 to 3,543,365 _3056 Position (kb) 3537 3538 3539 3540 3541 3542Strain fitness (log2 ratio) -3 -2 -1 0 1at 3541.391 kb on - strandat 3541.405 kb on - strandat 3541.449 kb on - strandat 3541.449 kb on - strandat 3541.537 kb on + strandat 3541.628 kb on + strandat 3541.629 kb on - strandat 3541.629 kb on - strandat 3541.749 kb on - strand, within Pf6N2E2_3055at 3541.882 kb on - strand, within Pf6N2E2_3055at 3542.036 kb on + strand, within Pf6N2E2_3055at 3542.221 kb on + strandat 3542.221 kb on + strandat 3542.221 kb on + strandat 3542.222 kb on - strandat 3542.222 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
remove
3,541,391 - +0.3
3,541,405 - +1.0
3,541,449 - +1.3
3,541,449 - -0.0
3,541,537 + -0.0
3,541,628 + -0.3
3,541,629 - +0.2
3,541,629 - -0.2
3,541,749 - Pf6N2E2_3055 0.26 +1.3
3,541,882 - Pf6N2E2_3055 0.47 +0.3
3,542,036 + Pf6N2E2_3055 0.71 +0.8
3,542,221 + -3.1
3,542,221 + +0.0
3,542,221 + +0.0
3,542,222 - +0.2
3,542,222 - -0.0

Or see this region's nucleotide sequence