Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1830 and Pf6N2E2_1831 are separated by 17 nucleotides Pf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides Pf6N2E2_1832 and Pf6N2E2_1833 overlap by 8 nucleotides
Pf6N2E2_1830 - Methylsuccinyl-CoA dehydrogenase, predicted by (Erb et al, 2007), at 2,167,717 to 2,169,387
_1830
Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304
_1831
Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158
_1832
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.387 kb on + strand at 2169.388 kb on - strand at 2169.388 kb on - strand at 2169.410 kb on + strand at 2169.411 kb on - strand at 2169.411 kb on - strand at 2169.888 kb on + strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2169.904 kb on - strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.088 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.104 kb on + strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.105 kb on - strand, within Pf6N2E2_1831 at 2170.253 kb on + strand at 2170.406 kb on + strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.463 kb on + strand, within Pf6N2E2_1832 at 2170.500 kb on - strand, within Pf6N2E2_1832 at 2170.572 kb on + strand, within Pf6N2E2_1832 at 2170.649 kb on - strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.996 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.137 kb on - strand at 2171.181 kb on + strand at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.657 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2172.079 kb on + strand, within Pf6N2E2_1833 at 2172.081 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,169,387 + -0.3 2,169,387 + +1.3 2,169,387 + +1.0 2,169,387 + +0.2 2,169,388 - +0.3 2,169,388 - -1.3 2,169,410 + -0.7 2,169,411 - -0.3 2,169,411 - +0.3 2,169,888 + Pf6N2E2_1831 0.54 -0.3 2,169,904 - Pf6N2E2_1831 0.55 -1.0 2,169,904 - Pf6N2E2_1831 0.55 +0.7 2,170,088 + Pf6N2E2_1831 0.76 +0.5 2,170,088 + Pf6N2E2_1831 0.76 -0.9 2,170,088 + Pf6N2E2_1831 0.76 -1.8 2,170,104 + Pf6N2E2_1831 0.78 -1.6 2,170,104 + Pf6N2E2_1831 0.78 +0.6 2,170,105 - Pf6N2E2_1831 0.78 -0.6 2,170,105 - Pf6N2E2_1831 0.78 -1.0 2,170,253 + -0.0 2,170,406 + Pf6N2E2_1832 0.12 -1.6 2,170,407 - Pf6N2E2_1832 0.12 -0.3 2,170,407 - Pf6N2E2_1832 0.12 -0.1 2,170,407 - Pf6N2E2_1832 0.12 +0.3 2,170,463 + Pf6N2E2_1832 0.19 -0.5 2,170,500 - Pf6N2E2_1832 0.23 -0.4 2,170,572 + Pf6N2E2_1832 0.32 -0.6 2,170,649 - Pf6N2E2_1832 0.41 +0.1 2,170,903 + Pf6N2E2_1832 0.70 -0.8 2,170,903 + Pf6N2E2_1832 0.70 -0.6 2,170,903 + Pf6N2E2_1832 0.70 -0.3 2,170,903 + Pf6N2E2_1832 0.70 -1.8 2,170,903 + Pf6N2E2_1832 0.70 +1.0 2,170,903 + Pf6N2E2_1832 0.70 -0.4 2,170,903 + Pf6N2E2_1832 0.70 -0.2 2,170,904 - Pf6N2E2_1832 0.70 +0.4 2,170,904 - Pf6N2E2_1832 0.70 +0.5 2,170,996 - Pf6N2E2_1832 0.81 +0.1 2,171,036 - Pf6N2E2_1832 0.86 +0.0 2,171,036 - Pf6N2E2_1832 0.86 -1.2 2,171,136 + +1.8 2,171,136 + +0.1 2,171,136 + -0.4 2,171,137 - +0.7 2,171,181 + +0.6 2,171,655 + Pf6N2E2_1833 0.33 -0.3 2,171,655 + Pf6N2E2_1833 0.33 -0.7 2,171,657 + Pf6N2E2_1833 0.33 +0.8 2,171,926 + Pf6N2E2_1833 0.51 -0.0 2,171,926 + Pf6N2E2_1833 0.51 -0.3 2,172,079 + Pf6N2E2_1833 0.61 -1.3 2,172,081 + Pf6N2E2_1833 0.61 -0.6 2,172,156 + Pf6N2E2_1833 0.66 -1.2 2,172,156 + Pf6N2E2_1833 0.66 -0.2
Or see this region's nucleotide sequence