Experiment: Mc formate, casamino free, with 10mM L-Methionine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04850 and MMP_RS04855 are separated by 5 nucleotides MMP_RS04855 and MMP_RS04860 are separated by 16 nucleotides MMP_RS04860 and MMP_RS04865 are separated by 53 nucleotides MMP_RS04865 and MMP_RS04870 are separated by 15 nucleotides
MMP_RS04850: MMP_RS04850 - UPF0254 family protein, at 923,434 to 923,931
_RS04850
MMP_RS04855: MMP_RS04855 - shikimate dehydrogenase, at 923,937 to 924,773
_RS04855
MMP_RS04860: MMP_RS04860 - coenzyme F420-0:L-glutamate ligase, at 924,790 to 925,536
_RS04860
MMP_RS04865: MMP_RS04865 - adenosylcobinamide-GDP ribazoletransferase, at 925,590 to 926,363
_RS04865
MMP_RS04870: MMP_RS04870 - TIGR02253 family HAD-type hydrolase, at 926,379 to 927,056
_RS04870
Position (kb) 924
925
926 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 923.791 kb on - strand, within MMP_RS04850 at 923.791 kb on - strand, within MMP_RS04850 at 923.801 kb on + strand, within MMP_RS04850 at 923.801 kb on + strand, within MMP_RS04850 at 923.801 kb on + strand, within MMP_RS04850 at 923.817 kb on - strand, within MMP_RS04850 at 923.818 kb on + strand, within MMP_RS04850 at 923.822 kb on + strand, within MMP_RS04850 at 923.832 kb on + strand, within MMP_RS04850 at 923.849 kb on - strand, within MMP_RS04850 at 923.850 kb on + strand, within MMP_RS04850 at 923.850 kb on + strand, within MMP_RS04850 at 923.850 kb on + strand, within MMP_RS04850 at 923.885 kb on + strand at 923.885 kb on + strand at 923.885 kb on + strand at 923.887 kb on + strand at 923.887 kb on + strand at 923.887 kb on + strand at 923.889 kb on - strand at 923.890 kb on + strand at 923.890 kb on + strand at 923.890 kb on + strand at 923.890 kb on + strand at 923.960 kb on + strand at 924.032 kb on - strand at 924.091 kb on - strand at 924.238 kb on - strand at 924.243 kb on - strand at 924.243 kb on - strand at 924.246 kb on + strand at 924.435 kb on + strand at 924.502 kb on - strand at 924.503 kb on + strand at 924.513 kb on + strand at 924.558 kb on + strand at 924.683 kb on + strand at 924.725 kb on + strand at 924.756 kb on + strand at 924.819 kb on + strand at 924.876 kb on - strand at 924.908 kb on - strand at 924.911 kb on - strand at 925.098 kb on - strand at 925.166 kb on - strand at 925.241 kb on - strand at 925.242 kb on + strand at 925.249 kb on + strand at 925.272 kb on - strand at 925.273 kb on + strand at 925.349 kb on - strand at 925.558 kb on + strand at 925.561 kb on - strand at 925.562 kb on + strand at 925.598 kb on - strand at 925.599 kb on + strand at 925.630 kb on + strand at 925.701 kb on + strand at 925.755 kb on - strand at 925.755 kb on - strand at 925.932 kb on - strand at 925.933 kb on + strand at 925.938 kb on + strand at 925.951 kb on + strand at 926.001 kb on - strand at 926.002 kb on + strand at 926.022 kb on - strand at 926.045 kb on + strand at 926.057 kb on - strand at 926.088 kb on - strand at 926.145 kb on + strand at 926.145 kb on + strand at 926.147 kb on + strand at 926.148 kb on - strand at 926.213 kb on - strand at 926.359 kb on + strand at 926.384 kb on - strand at 926.385 kb on + strand at 926.393 kb on - strand at 926.417 kb on - strand at 926.431 kb on - strand at 926.432 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.517 kb on + strand, within MMP_RS04870
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM L-Methionine remove 923,791 - MMP_RS04850 0.72 +1.8 923,791 - MMP_RS04850 0.72 +2.0 923,801 + MMP_RS04850 0.74 +2.2 923,801 + MMP_RS04850 0.74 +2.1 923,801 + MMP_RS04850 0.74 +0.9 923,817 - MMP_RS04850 0.77 +1.8 923,818 + MMP_RS04850 0.77 +0.7 923,822 + MMP_RS04850 0.78 +0.5 923,832 + MMP_RS04850 0.80 +1.1 923,849 - MMP_RS04850 0.83 -0.1 923,850 + MMP_RS04850 0.84 +0.9 923,850 + MMP_RS04850 0.84 -0.3 923,850 + MMP_RS04850 0.84 +1.0 923,885 + +1.3 923,885 + +1.4 923,885 + +0.8 923,887 + +0.4 923,887 + +1.8 923,887 + +1.9 923,889 - +0.5 923,890 + +0.4 923,890 + +0.3 923,890 + -1.4 923,890 + +2.2 923,960 + -2.8 924,032 - -1.6 924,091 - -2.1 924,238 - -0.5 924,243 - -2.9 924,243 - -3.5 924,246 + -0.4 924,435 + -0.7 924,502 - -1.8 924,503 + -0.8 924,513 + -4.1 924,558 + -1.5 924,683 + +0.9 924,725 + -3.1 924,756 + -0.5 924,819 + -2.7 924,876 - -0.7 924,908 - -1.2 924,911 - -1.4 925,098 - -2.3 925,166 - -0.5 925,241 - -2.3 925,242 + -3.5 925,249 + -0.7 925,272 - -3.6 925,273 + -2.3 925,349 - -1.5 925,558 + -0.7 925,561 - +0.0 925,562 + +0.0 925,598 - -0.6 925,599 + -1.1 925,630 + -2.6 925,701 + -3.0 925,755 - -1.8 925,755 - -2.9 925,932 - -2.3 925,933 + -4.5 925,938 + -4.9 925,951 + -2.1 926,001 - -2.4 926,002 + -2.2 926,022 - -1.9 926,045 + -1.1 926,057 - -2.0 926,088 - -1.9 926,145 + -1.9 926,145 + -4.3 926,147 + -2.1 926,148 - -0.8 926,213 - -1.4 926,359 + -2.1 926,384 - -0.4 926,385 + +0.1 926,393 - -1.4 926,417 - -3.0 926,431 - -2.9 926,432 + -1.9 926,440 + -2.7 926,440 + -3.3 926,440 + -3.9 926,517 + MMP_RS04870 0.20 -2.0
Or see this region's nucleotide sequence