Experiment: BHIS with Anhydrotetracycline 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2303 and BT2304 are separated by 372 nucleotides BT2304 and BT2305 are separated by 152 nucleotides
BT2303: BT2303 - conserved protein found in conjugate transposon (NCBI ptt file), at 2,877,418 to 2,878,176
BT2303
BT2304: BT2304 - hypothetical protein (NCBI ptt file), at 2,878,549 to 2,879,244
BT2304
BT2305: BT2305 - putative mobilization protein (NCBI ptt file), at 2,879,397 to 2,880,623
BT2305
Position (kb)
2878
2879
2880 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2877.623 kb on + strand, within BT2303 at 2877.624 kb on - strand, within BT2303 at 2877.668 kb on + strand, within BT2303 at 2877.782 kb on + strand, within BT2303 at 2877.782 kb on + strand, within BT2303 at 2878.091 kb on + strand, within BT2303 at 2878.190 kb on + strand at 2878.324 kb on + strand at 2878.324 kb on + strand at 2878.520 kb on - strand at 2878.688 kb on - strand, within BT2304 at 2878.688 kb on - strand, within BT2304 at 2878.689 kb on + strand, within BT2304 at 2878.817 kb on + strand, within BT2304 at 2878.818 kb on - strand, within BT2304 at 2878.956 kb on - strand, within BT2304 at 2878.962 kb on + strand, within BT2304 at 2878.971 kb on + strand, within BT2304 at 2878.971 kb on + strand, within BT2304 at 2878.974 kb on - strand, within BT2304 at 2879.206 kb on - strand at 2879.319 kb on - strand at 2879.445 kb on + strand at 2879.445 kb on + strand at 2879.446 kb on - strand at 2879.596 kb on + strand, within BT2305 at 2879.597 kb on - strand, within BT2305 at 2879.597 kb on - strand, within BT2305 at 2879.965 kb on - strand, within BT2305 at 2880.010 kb on - strand, within BT2305 at 2880.011 kb on + strand, within BT2305 at 2880.016 kb on + strand, within BT2305 at 2880.057 kb on + strand, within BT2305 at 2880.067 kb on + strand, within BT2305 at 2880.068 kb on - strand, within BT2305 at 2880.068 kb on - strand, within BT2305
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Anhydrotetracycline 0.0125 mM remove 2,877,623 + BT2303 0.27 -2.0 2,877,624 - BT2303 0.27 -1.6 2,877,668 + BT2303 0.33 -1.0 2,877,782 + BT2303 0.48 -0.6 2,877,782 + BT2303 0.48 -1.1 2,878,091 + BT2303 0.89 -1.2 2,878,190 + -0.0 2,878,324 + +1.0 2,878,324 + -0.8 2,878,520 - -1.0 2,878,688 - BT2304 0.20 -1.6 2,878,688 - BT2304 0.20 -1.5 2,878,689 + BT2304 0.20 -1.0 2,878,817 + BT2304 0.39 -1.0 2,878,818 - BT2304 0.39 -1.0 2,878,956 - BT2304 0.58 -3.5 2,878,962 + BT2304 0.59 -2.6 2,878,971 + BT2304 0.61 -2.2 2,878,971 + BT2304 0.61 -1.0 2,878,974 - BT2304 0.61 -2.2 2,879,206 - -1.2 2,879,319 - -1.1 2,879,445 + -1.3 2,879,445 + -0.0 2,879,446 - -0.0 2,879,596 + BT2305 0.16 -2.6 2,879,597 - BT2305 0.16 -3.0 2,879,597 - BT2305 0.16 -0.6 2,879,965 - BT2305 0.46 -0.6 2,880,010 - BT2305 0.50 -0.6 2,880,011 + BT2305 0.50 -2.3 2,880,016 + BT2305 0.50 -2.6 2,880,057 + BT2305 0.54 -0.0 2,880,067 + BT2305 0.55 -2.2 2,880,068 - BT2305 0.55 -0.5 2,880,068 - BT2305 0.55 -0.7
Or see this region's nucleotide sequence