Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18940 and BWI76_RS18945 overlap by 1 nucleotides BWI76_RS18945 and BWI76_RS18950 overlap by 19 nucleotides BWI76_RS18950 and BWI76_RS18955 overlap by 7 nucleotides BWI76_RS18955 and BWI76_RS18960 are separated by 83 nucleotides
BWI76_RS18940: BWI76_RS18940 - imidazole glycerol phosphate synthase subunit HisH, at 3,928,964 to 3,929,554
_RS18940
BWI76_RS18945: BWI76_RS18945 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 3,929,554 to 3,930,291
_RS18945
BWI76_RS18950: BWI76_RS18950 - imidazole glycerol phosphate synthase cyclase subunit, at 3,930,273 to 3,931,049
_RS18950
BWI76_RS18955: BWI76_RS18955 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase, at 3,931,043 to 3,931,642
_RS18955
BWI76_RS18960: BWI76_RS18960 - glycosyl transferase, at 3,931,726 to 3,933,045
_RS18960
Position (kb) 3930
3931
3932 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 3929.368 kb on - strand, within BWI76_RS18940 at 3929.441 kb on + strand, within BWI76_RS18940 at 3929.476 kb on + strand, within BWI76_RS18940 at 3930.057 kb on + strand, within BWI76_RS18945 at 3930.223 kb on - strand at 3930.377 kb on + strand, within BWI76_RS18950 at 3930.426 kb on + strand, within BWI76_RS18950 at 3930.445 kb on + strand, within BWI76_RS18950 at 3930.909 kb on + strand, within BWI76_RS18950 at 3931.121 kb on + strand, within BWI76_RS18955 at 3931.498 kb on - strand, within BWI76_RS18955 at 3931.509 kb on + strand, within BWI76_RS18955 at 3931.517 kb on + strand, within BWI76_RS18955 at 3931.517 kb on + strand, within BWI76_RS18955 at 3931.640 kb on - strand at 3931.653 kb on - strand at 3931.674 kb on + strand at 3931.723 kb on - strand at 3931.762 kb on + strand at 3931.770 kb on - strand at 3931.816 kb on - strand at 3931.816 kb on - strand at 3931.910 kb on - strand, within BWI76_RS18960 at 3931.936 kb on + strand, within BWI76_RS18960 at 3932.013 kb on + strand, within BWI76_RS18960 at 3932.036 kb on + strand, within BWI76_RS18960 at 3932.045 kb on - strand, within BWI76_RS18960
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 3,929,368 - BWI76_RS18940 0.68 -6.4 3,929,441 + BWI76_RS18940 0.81 -4.4 3,929,476 + BWI76_RS18940 0.87 -3.1 3,930,057 + BWI76_RS18945 0.68 -1.6 3,930,223 - -3.1 3,930,377 + BWI76_RS18950 0.13 -5.0 3,930,426 + BWI76_RS18950 0.20 -2.9 3,930,445 + BWI76_RS18950 0.22 -5.1 3,930,909 + BWI76_RS18950 0.82 -3.6 3,931,121 + BWI76_RS18955 0.13 -5.6 3,931,498 - BWI76_RS18955 0.76 -5.4 3,931,509 + BWI76_RS18955 0.78 -1.7 3,931,517 + BWI76_RS18955 0.79 -4.7 3,931,517 + BWI76_RS18955 0.79 -3.3 3,931,640 - -0.3 3,931,653 - -0.3 3,931,674 + -0.6 3,931,723 - +0.3 3,931,762 + +0.6 3,931,770 - -0.4 3,931,816 - +0.7 3,931,816 - -0.2 3,931,910 - BWI76_RS18960 0.14 +0.7 3,931,936 + BWI76_RS18960 0.16 -0.4 3,932,013 + BWI76_RS18960 0.22 +0.3 3,932,036 + BWI76_RS18960 0.23 +1.7 3,932,045 - BWI76_RS18960 0.24 +0.2
Or see this region's nucleotide sequence