Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14025

Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14020 and BWI76_RS14025 are separated by 307 nucleotidesBWI76_RS14025 and BWI76_RS14030 are separated by 24 nucleotidesBWI76_RS14030 and BWI76_RS14035 overlap by 4 nucleotides BWI76_RS14020: BWI76_RS14020 - aspartate aminotransferase family protein, at 2,929,847 to 2,931,232 _RS14020 BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475 _RS14025 BWI76_RS14030: BWI76_RS14030 - ABC transporter permease, at 2,932,500 to 2,933,240 _RS14030 BWI76_RS14035: BWI76_RS14035 - ABC transporter ATPase, at 2,933,237 to 2,933,965 _RS14035 Position (kb) 2931 2932 2933Strain fitness (log2 ratio) -2 -1 0 1at 2930.675 kb on - strand, within BWI76_RS14020at 2931.084 kb on + strand, within BWI76_RS14020at 2931.084 kb on - strand, within BWI76_RS14020at 2931.084 kb on - strand, within BWI76_RS14020at 2931.152 kb on - strandat 2931.185 kb on + strandat 2931.185 kb on + strandat 2931.239 kb on + strandat 2931.239 kb on + strandat 2931.241 kb on + strandat 2931.245 kb on - strandat 2931.247 kb on - strandat 2931.247 kb on - strandat 2931.247 kb on - strandat 2931.249 kb on + strandat 2931.249 kb on + strandat 2931.249 kb on + strandat 2931.257 kb on - strandat 2931.257 kb on - strandat 2931.260 kb on - strandat 2931.265 kb on - strandat 2931.265 kb on - strandat 2931.311 kb on - strandat 2931.320 kb on + strandat 2931.320 kb on + strandat 2931.328 kb on - strandat 2931.377 kb on + strandat 2931.377 kb on + strandat 2931.386 kb on - strandat 2931.533 kb on + strandat 2931.538 kb on + strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.588 kb on - strandat 2931.589 kb on + strandat 2931.600 kb on + strandat 2931.601 kb on + strandat 2931.619 kb on - strandat 2931.620 kb on - strandat 2931.639 kb on - strand, within BWI76_RS14025at 2931.665 kb on + strand, within BWI76_RS14025at 2931.672 kb on + strand, within BWI76_RS14025at 2931.703 kb on + strand, within BWI76_RS14025at 2931.703 kb on + strand, within BWI76_RS14025at 2931.711 kb on - strand, within BWI76_RS14025at 2931.734 kb on + strand, within BWI76_RS14025at 2931.772 kb on - strand, within BWI76_RS14025at 2931.898 kb on + strand, within BWI76_RS14025at 2932.013 kb on - strand, within BWI76_RS14025at 2932.197 kb on + strand, within BWI76_RS14025at 2932.483 kb on - strandat 2932.527 kb on + strandat 2932.563 kb on + strandat 2932.789 kb on - strand, within BWI76_RS14030at 2932.819 kb on + strand, within BWI76_RS14030at 2933.013 kb on - strand, within BWI76_RS14030at 2933.034 kb on - strand, within BWI76_RS14030at 2933.228 kb on + strandat 2933.236 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
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2,930,675 - BWI76_RS14020 0.60 +0.1
2,931,084 + BWI76_RS14020 0.89 +0.0
2,931,084 - BWI76_RS14020 0.89 +0.7
2,931,084 - BWI76_RS14020 0.89 -1.3
2,931,152 - +1.5
2,931,185 + -0.0
2,931,185 + +0.3
2,931,239 + -0.0
2,931,239 + +1.5
2,931,241 + -0.7
2,931,245 - +0.3
2,931,247 - +0.0
2,931,247 - -0.1
2,931,247 - -0.4
2,931,249 + -0.3
2,931,249 + +0.2
2,931,249 + -0.4
2,931,257 - +0.3
2,931,257 - -0.0
2,931,260 - -0.2
2,931,265 - -0.8
2,931,265 - -0.4
2,931,311 - -0.0
2,931,320 + +0.4
2,931,320 + -1.8
2,931,328 - -0.2
2,931,377 + -1.3
2,931,377 + -0.1
2,931,386 - -0.6
2,931,533 + +0.2
2,931,538 + -0.2
2,931,546 - -0.5
2,931,546 - -0.8
2,931,546 - -0.1
2,931,546 - +0.5
2,931,588 - -0.3
2,931,589 + -0.1
2,931,600 + +0.3
2,931,601 + +0.2
2,931,619 - +0.2
2,931,620 - -0.1
2,931,639 - BWI76_RS14025 0.11 +0.0
2,931,665 + BWI76_RS14025 0.13 +0.1
2,931,672 + BWI76_RS14025 0.14 +0.3
2,931,703 + BWI76_RS14025 0.17 +0.1
2,931,703 + BWI76_RS14025 0.17 +0.1
2,931,711 - BWI76_RS14025 0.18 -0.1
2,931,734 + BWI76_RS14025 0.21 +0.2
2,931,772 - BWI76_RS14025 0.25 +0.4
2,931,898 + BWI76_RS14025 0.38 -0.3
2,932,013 - BWI76_RS14025 0.51 +0.0
2,932,197 + BWI76_RS14025 0.70 +0.1
2,932,483 - -0.2
2,932,527 + -0.0
2,932,563 + +0.1
2,932,789 - BWI76_RS14030 0.39 +0.5
2,932,819 + BWI76_RS14030 0.43 +0.0
2,933,013 - BWI76_RS14030 0.69 +0.1
2,933,034 - BWI76_RS14030 0.72 +0.2
2,933,228 + -0.2
2,933,236 - +0.3

Or see this region's nucleotide sequence