Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14020 and BWI76_RS14025 are separated by 307 nucleotides BWI76_RS14025 and BWI76_RS14030 are separated by 24 nucleotides BWI76_RS14030 and BWI76_RS14035 overlap by 4 nucleotides
BWI76_RS14020: BWI76_RS14020 - aspartate aminotransferase family protein, at 2,929,847 to 2,931,232
_RS14020
BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475
_RS14025
BWI76_RS14030: BWI76_RS14030 - ABC transporter permease, at 2,932,500 to 2,933,240
_RS14030
BWI76_RS14035: BWI76_RS14035 - ABC transporter ATPase, at 2,933,237 to 2,933,965
_RS14035
Position (kb)
2931
2932
2933 Strain fitness (log2 ratio)
-2
-1
0
1 at 2930.675 kb on - strand, within BWI76_RS14020 at 2931.084 kb on + strand, within BWI76_RS14020 at 2931.084 kb on - strand, within BWI76_RS14020 at 2931.084 kb on - strand, within BWI76_RS14020 at 2931.152 kb on - strand at 2931.185 kb on + strand at 2931.185 kb on + strand at 2931.239 kb on + strand at 2931.239 kb on + strand at 2931.241 kb on + strand at 2931.245 kb on - strand at 2931.247 kb on - strand at 2931.247 kb on - strand at 2931.247 kb on - strand at 2931.249 kb on + strand at 2931.249 kb on + strand at 2931.249 kb on + strand at 2931.257 kb on - strand at 2931.257 kb on - strand at 2931.260 kb on - strand at 2931.265 kb on - strand at 2931.265 kb on - strand at 2931.311 kb on - strand at 2931.320 kb on + strand at 2931.320 kb on + strand at 2931.328 kb on - strand at 2931.377 kb on + strand at 2931.377 kb on + strand at 2931.386 kb on - strand at 2931.533 kb on + strand at 2931.538 kb on + strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.588 kb on - strand at 2931.589 kb on + strand at 2931.600 kb on + strand at 2931.601 kb on + strand at 2931.619 kb on - strand at 2931.620 kb on - strand at 2931.639 kb on - strand, within BWI76_RS14025 at 2931.665 kb on + strand, within BWI76_RS14025 at 2931.672 kb on + strand, within BWI76_RS14025 at 2931.703 kb on + strand, within BWI76_RS14025 at 2931.703 kb on + strand, within BWI76_RS14025 at 2931.711 kb on - strand, within BWI76_RS14025 at 2931.734 kb on + strand, within BWI76_RS14025 at 2931.772 kb on - strand, within BWI76_RS14025 at 2931.898 kb on + strand, within BWI76_RS14025 at 2932.013 kb on - strand, within BWI76_RS14025 at 2932.197 kb on + strand, within BWI76_RS14025 at 2932.483 kb on - strand at 2932.527 kb on + strand at 2932.563 kb on + strand at 2932.789 kb on - strand, within BWI76_RS14030 at 2932.819 kb on + strand, within BWI76_RS14030 at 2933.013 kb on - strand, within BWI76_RS14030 at 2933.034 kb on - strand, within BWI76_RS14030 at 2933.228 kb on + strand at 2933.236 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 2,930,675 - BWI76_RS14020 0.60 +0.1 2,931,084 + BWI76_RS14020 0.89 +0.0 2,931,084 - BWI76_RS14020 0.89 +0.7 2,931,084 - BWI76_RS14020 0.89 -1.3 2,931,152 - +1.5 2,931,185 + -0.0 2,931,185 + +0.3 2,931,239 + -0.0 2,931,239 + +1.5 2,931,241 + -0.7 2,931,245 - +0.3 2,931,247 - +0.0 2,931,247 - -0.1 2,931,247 - -0.4 2,931,249 + -0.3 2,931,249 + +0.2 2,931,249 + -0.4 2,931,257 - +0.3 2,931,257 - -0.0 2,931,260 - -0.2 2,931,265 - -0.8 2,931,265 - -0.4 2,931,311 - -0.0 2,931,320 + +0.4 2,931,320 + -1.8 2,931,328 - -0.2 2,931,377 + -1.3 2,931,377 + -0.1 2,931,386 - -0.6 2,931,533 + +0.2 2,931,538 + -0.2 2,931,546 - -0.5 2,931,546 - -0.8 2,931,546 - -0.1 2,931,546 - +0.5 2,931,588 - -0.3 2,931,589 + -0.1 2,931,600 + +0.3 2,931,601 + +0.2 2,931,619 - +0.2 2,931,620 - -0.1 2,931,639 - BWI76_RS14025 0.11 +0.0 2,931,665 + BWI76_RS14025 0.13 +0.1 2,931,672 + BWI76_RS14025 0.14 +0.3 2,931,703 + BWI76_RS14025 0.17 +0.1 2,931,703 + BWI76_RS14025 0.17 +0.1 2,931,711 - BWI76_RS14025 0.18 -0.1 2,931,734 + BWI76_RS14025 0.21 +0.2 2,931,772 - BWI76_RS14025 0.25 +0.4 2,931,898 + BWI76_RS14025 0.38 -0.3 2,932,013 - BWI76_RS14025 0.51 +0.0 2,932,197 + BWI76_RS14025 0.70 +0.1 2,932,483 - -0.2 2,932,527 + -0.0 2,932,563 + +0.1 2,932,789 - BWI76_RS14030 0.39 +0.5 2,932,819 + BWI76_RS14030 0.43 +0.0 2,933,013 - BWI76_RS14030 0.69 +0.1 2,933,034 - BWI76_RS14030 0.72 +0.2 2,933,228 + -0.2 2,933,236 - +0.3
Or see this region's nucleotide sequence