Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06355 and BWI76_RS06360 are separated by 19 nucleotides BWI76_RS06360 and BWI76_RS06365 are separated by 75 nucleotides BWI76_RS06365 and BWI76_RS06370 overlap by 14 nucleotides BWI76_RS06370 and BWI76_RS06375 are separated by 289 nucleotides
BWI76_RS06355: BWI76_RS06355 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,336,829 to 1,337,299
_RS06355
BWI76_RS06360: BWI76_RS06360 - N utilization substance protein B, at 1,337,319 to 1,337,738
_RS06360
BWI76_RS06365: BWI76_RS06365 - thiamine-monophosphate kinase, at 1,337,814 to 1,338,791
_RS06365
BWI76_RS06370: BWI76_RS06370 - phosphatidylglycerophosphatase A, at 1,338,778 to 1,339,284
_RS06370
BWI76_RS06375: BWI76_RS06375 - oxidoreductase, at 1,339,574 to 1,340,548
_RS06375
Position (kb) 1337
1338
1339 Strain fitness (log2 ratio) -1
0
1 at 1338.855 kb on - strand, within BWI76_RS06370 at 1338.982 kb on - strand, within BWI76_RS06370 at 1339.011 kb on - strand, within BWI76_RS06370 at 1339.050 kb on + strand, within BWI76_RS06370 at 1339.222 kb on + strand, within BWI76_RS06370 at 1339.255 kb on + strand at 1339.260 kb on + strand at 1339.398 kb on - strand at 1339.404 kb on - strand at 1339.426 kb on + strand at 1339.523 kb on - strand at 1339.611 kb on + strand at 1339.630 kb on + strand at 1339.718 kb on + strand, within BWI76_RS06375 at 1339.739 kb on - strand, within BWI76_RS06375
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 1,338,855 - BWI76_RS06370 0.15 -0.1 1,338,982 - BWI76_RS06370 0.40 +0.1 1,339,011 - BWI76_RS06370 0.46 -0.2 1,339,050 + BWI76_RS06370 0.54 -0.0 1,339,222 + BWI76_RS06370 0.88 -0.2 1,339,255 + -0.3 1,339,260 + -0.8 1,339,398 - +0.3 1,339,404 - +0.0 1,339,426 + -0.5 1,339,523 - -0.5 1,339,611 + -0.8 1,339,630 + -0.4 1,339,718 + BWI76_RS06375 0.15 -0.3 1,339,739 - BWI76_RS06375 0.17 -0.1
Or see this region's nucleotide sequence