Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00950 and BWI76_RS00955 are separated by 349 nucleotides BWI76_RS00955 and BWI76_RS00960 are separated by 139 nucleotides BWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotides BWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotides
BWI76_RS00950: BWI76_RS00950 - ATP-dependent protease, at 193,279 to 194,799
_RS00950
BWI76_RS00955: BWI76_RS00955 - IlvGMEDA operon leader peptide, at 195,149 to 195,247
_RS00955
BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033
_RS00960
BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287
_RS00965
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 194.428 kb on + strand, within BWI76_RS00950 at 194.517 kb on + strand, within BWI76_RS00950 at 194.525 kb on - strand, within BWI76_RS00950 at 194.656 kb on - strand at 194.699 kb on - strand at 194.748 kb on + strand at 194.769 kb on - strand at 194.784 kb on - strand at 194.948 kb on + strand at 195.501 kb on + strand at 195.528 kb on - strand at 195.528 kb on - strand at 195.565 kb on - strand, within BWI76_RS00960 at 195.595 kb on + strand, within BWI76_RS00960 at 195.642 kb on - strand at 195.652 kb on - strand, within BWI76_RS00960 at 195.841 kb on - strand, within BWI76_RS00960 at 196.023 kb on + strand, within BWI76_RS00960 at 196.149 kb on - strand, within BWI76_RS00960 at 196.149 kb on - strand, within BWI76_RS00960 at 196.541 kb on - strand, within BWI76_RS00960 at 196.597 kb on + strand at 196.605 kb on - strand, within BWI76_RS00960 at 196.754 kb on + strand, within BWI76_RS00960 at 196.869 kb on + strand at 196.933 kb on + strand at 196.941 kb on - strand at 196.973 kb on + strand at 196.981 kb on - strand at 197.236 kb on - strand, within BWI76_RS00965 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 194,428 + BWI76_RS00950 0.76 +0.9 194,517 + BWI76_RS00950 0.81 -0.1 194,525 - BWI76_RS00950 0.82 +0.2 194,656 - -0.0 194,699 - -0.2 194,748 + -0.5 194,769 - -1.2 194,784 - +0.0 194,948 + -0.2 195,501 + -4.4 195,528 - -3.5 195,528 - -2.3 195,565 - BWI76_RS00960 0.11 -4.5 195,595 + BWI76_RS00960 0.13 -5.0 195,642 - -1.0 195,652 - BWI76_RS00960 0.16 -4.0 195,841 - BWI76_RS00960 0.28 -3.8 196,023 + BWI76_RS00960 0.39 -3.8 196,149 - BWI76_RS00960 0.46 -2.4 196,149 - BWI76_RS00960 0.46 -3.0 196,541 - BWI76_RS00960 0.70 -4.2 196,597 + +0.7 196,605 - BWI76_RS00960 0.74 -2.3 196,754 + BWI76_RS00960 0.83 -1.1 196,869 + -1.4 196,933 + -1.9 196,941 - -3.8 196,973 + -1.0 196,981 - -2.9 197,236 - BWI76_RS00965 0.80 -2.3 197,330 - -3.2 197,383 + -2.7 197,399 - BWI76_RS00970 0.10 -4.5 197,484 + BWI76_RS00970 0.19 -1.4 197,484 + BWI76_RS00970 0.19 -1.1 197,540 + BWI76_RS00970 0.25 -2.7 197,540 + BWI76_RS00970 0.25 -3.3 197,686 + BWI76_RS00970 0.41 -0.9
Or see this region's nucleotide sequence