Experiment: Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00785 and BWI76_RS00790 are separated by 2 nucleotides BWI76_RS00790 and BWI76_RS00795 are separated by 39 nucleotides BWI76_RS00795 and BWI76_RS00800 are separated by 91 nucleotides
BWI76_RS00785: BWI76_RS00785 - cystathionine gamma-synthase, at 159,813 to 160,973
_RS00785
BWI76_RS00790: BWI76_RS00790 - bifunctional aspartokinase II/homoserine dehydrogenase II, at 160,976 to 163,408
_RS00790
BWI76_RS00795: BWI76_RS00795 - carboxymuconolactone decarboxylase, at 163,448 to 163,819
_RS00795
BWI76_RS00800: BWI76_RS00800 - LysR family transcriptional regulator, at 163,911 to 164,816
_RS00800
Position (kb) 160
161
162
163
164 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 160.023 kb on + strand, within BWI76_RS00785 at 160.223 kb on + strand, within BWI76_RS00785 at 160.262 kb on + strand, within BWI76_RS00785 at 160.277 kb on - strand, within BWI76_RS00785 at 160.337 kb on - strand, within BWI76_RS00785 at 160.423 kb on + strand at 160.526 kb on - strand, within BWI76_RS00785 at 160.546 kb on + strand, within BWI76_RS00785 at 160.575 kb on + strand, within BWI76_RS00785 at 160.575 kb on + strand, within BWI76_RS00785 at 160.607 kb on + strand, within BWI76_RS00785 at 160.770 kb on - strand, within BWI76_RS00785 at 160.906 kb on + strand at 160.906 kb on + strand at 160.906 kb on + strand at 161.001 kb on - strand at 161.015 kb on - strand at 161.424 kb on + strand, within BWI76_RS00790 at 161.587 kb on + strand, within BWI76_RS00790 at 161.635 kb on + strand, within BWI76_RS00790 at 161.852 kb on - strand, within BWI76_RS00790 at 161.866 kb on + strand, within BWI76_RS00790 at 162.077 kb on - strand, within BWI76_RS00790 at 162.134 kb on - strand, within BWI76_RS00790 at 162.769 kb on + strand, within BWI76_RS00790 at 162.812 kb on - strand, within BWI76_RS00790 at 162.843 kb on + strand, within BWI76_RS00790 at 162.911 kb on - strand, within BWI76_RS00790 at 163.095 kb on + strand, within BWI76_RS00790 at 163.183 kb on + strand at 163.191 kb on - strand at 163.379 kb on + strand at 163.613 kb on - strand, within BWI76_RS00795 at 163.650 kb on - strand, within BWI76_RS00795 at 163.740 kb on - strand, within BWI76_RS00795 at 163.778 kb on + strand, within BWI76_RS00795 at 163.799 kb on + strand at 163.822 kb on - strand at 163.839 kb on - strand at 163.866 kb on + strand at 163.889 kb on - strand at 164.060 kb on + strand at 164.154 kb on + strand, within BWI76_RS00800 at 164.233 kb on + strand, within BWI76_RS00800 at 164.241 kb on - strand, within BWI76_RS00800 at 164.241 kb on - strand, within BWI76_RS00800
Per-strain Table
Position Strand Gene LocusTag Fraction Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM remove 160,023 + BWI76_RS00785 0.18 -4.1 160,223 + BWI76_RS00785 0.35 -2.7 160,262 + BWI76_RS00785 0.39 -3.0 160,277 - BWI76_RS00785 0.40 -2.8 160,337 - BWI76_RS00785 0.45 -3.5 160,423 + +3.0 160,526 - BWI76_RS00785 0.61 -3.6 160,546 + BWI76_RS00785 0.63 -4.3 160,575 + BWI76_RS00785 0.66 -0.3 160,575 + BWI76_RS00785 0.66 -4.5 160,607 + BWI76_RS00785 0.68 -0.6 160,770 - BWI76_RS00785 0.82 -5.7 160,906 + -3.2 160,906 + -5.7 160,906 + -2.1 161,001 - -0.0 161,015 - -1.6 161,424 + BWI76_RS00790 0.18 -0.4 161,587 + BWI76_RS00790 0.25 -1.2 161,635 + BWI76_RS00790 0.27 +0.4 161,852 - BWI76_RS00790 0.36 +0.1 161,866 + BWI76_RS00790 0.37 -0.7 162,077 - BWI76_RS00790 0.45 -1.2 162,134 - BWI76_RS00790 0.48 +0.5 162,769 + BWI76_RS00790 0.74 -0.9 162,812 - BWI76_RS00790 0.75 -0.2 162,843 + BWI76_RS00790 0.77 +0.2 162,911 - BWI76_RS00790 0.80 +0.4 163,095 + BWI76_RS00790 0.87 +0.3 163,183 + +2.0 163,191 - +0.5 163,379 + +0.6 163,613 - BWI76_RS00795 0.44 +2.4 163,650 - BWI76_RS00795 0.54 +0.7 163,740 - BWI76_RS00795 0.78 +0.4 163,778 + BWI76_RS00795 0.89 -0.6 163,799 + +0.6 163,822 - -0.2 163,839 - -0.1 163,866 + +0.1 163,889 - -0.8 164,060 + +1.3 164,154 + BWI76_RS00800 0.27 -0.3 164,233 + BWI76_RS00800 0.36 -0.3 164,241 - BWI76_RS00800 0.36 -0.6 164,241 - BWI76_RS00800 0.36 -0.3
Or see this region's nucleotide sequence