Experiment: BHIS with Thioridazine 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4430 and BT4431 are separated by 195 nucleotides BT4431 and BT4432 are separated by 4 nucleotides
BT4430: BT4430 - cytochrome c biogenesis protein (ccsA) (NCBI ptt file), at 5,835,424 to 5,837,859
BT4430
BT4431: BT4431 - hypothetical protein (NCBI ptt file), at 5,838,055 to 5,839,854
BT4431
BT4432: BT4432 - putative non-specific DNA-binding protein HU-1 (NCBI ptt file), at 5,839,859 to 5,840,272
BT4432
Position (kb)
5838
5839
5840 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5837.137 kb on + strand, within BT4430 at 5837.232 kb on - strand, within BT4430 at 5837.283 kb on - strand, within BT4430 at 5837.283 kb on - strand, within BT4430 at 5837.312 kb on - strand, within BT4430 at 5837.354 kb on + strand, within BT4430 at 5837.462 kb on + strand, within BT4430 at 5837.462 kb on + strand, within BT4430 at 5837.463 kb on - strand, within BT4430 at 5837.492 kb on + strand, within BT4430 at 5837.596 kb on - strand, within BT4430 at 5837.596 kb on - strand, within BT4430 at 5837.605 kb on + strand, within BT4430 at 5837.650 kb on - strand at 5837.666 kb on - strand at 5837.726 kb on - strand at 5837.851 kb on + strand at 5837.869 kb on + strand at 5837.913 kb on - strand at 5838.006 kb on - strand at 5838.109 kb on + strand at 5838.119 kb on + strand at 5838.175 kb on - strand at 5838.175 kb on - strand at 5838.246 kb on + strand, within BT4431 at 5838.248 kb on + strand, within BT4431 at 5838.324 kb on + strand, within BT4431 at 5838.374 kb on + strand, within BT4431 at 5838.375 kb on - strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.376 kb on + strand, within BT4431 at 5838.377 kb on - strand, within BT4431 at 5838.379 kb on - strand, within BT4431 at 5838.381 kb on - strand, within BT4431 at 5838.547 kb on - strand, within BT4431 at 5838.635 kb on + strand, within BT4431 at 5838.635 kb on + strand, within BT4431 at 5838.720 kb on - strand, within BT4431 at 5838.745 kb on + strand, within BT4431 at 5838.763 kb on + strand, within BT4431 at 5838.853 kb on - strand, within BT4431 at 5838.971 kb on + strand, within BT4431 at 5838.972 kb on - strand, within BT4431 at 5839.015 kb on + strand, within BT4431 at 5839.015 kb on + strand, within BT4431 at 5839.016 kb on - strand, within BT4431 at 5839.016 kb on - strand, within BT4431 at 5839.116 kb on - strand, within BT4431 at 5839.116 kb on - strand, within BT4431 at 5839.154 kb on - strand, within BT4431 at 5839.154 kb on - strand, within BT4431 at 5839.195 kb on - strand, within BT4431 at 5839.196 kb on - strand, within BT4431 at 5839.209 kb on + strand, within BT4431 at 5839.209 kb on + strand, within BT4431 at 5839.269 kb on + strand, within BT4431 at 5839.493 kb on + strand, within BT4431 at 5839.493 kb on + strand, within BT4431 at 5839.494 kb on - strand, within BT4431 at 5839.497 kb on + strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.498 kb on - strand, within BT4431 at 5839.504 kb on - strand, within BT4431 at 5839.529 kb on - strand, within BT4431 at 5839.624 kb on - strand, within BT4431 at 5839.676 kb on - strand at 5839.693 kb on - strand at 5840.133 kb on - strand, within BT4432 at 5840.160 kb on - strand, within BT4432 at 5840.160 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.222 kb on - strand, within BT4432 at 5840.265 kb on - strand at 5840.276 kb on - strand at 5840.346 kb on - strand at 5840.426 kb on - strand at 5840.454 kb on - strand at 5840.509 kb on - strand at 5840.650 kb on - strand at 5840.680 kb on - strand at 5840.680 kb on - strand at 5840.683 kb on - strand at 5840.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Thioridazine 0.0125 mM remove 5,837,137 + BT4430 0.70 -1.1 5,837,232 - BT4430 0.74 +0.3 5,837,283 - BT4430 0.76 +0.0 5,837,283 - BT4430 0.76 +1.4 5,837,312 - BT4430 0.78 -0.7 5,837,354 + BT4430 0.79 -0.6 5,837,462 + BT4430 0.84 -1.7 5,837,462 + BT4430 0.84 -0.2 5,837,463 - BT4430 0.84 -1.0 5,837,492 + BT4430 0.85 -1.6 5,837,596 - BT4430 0.89 -0.6 5,837,596 - BT4430 0.89 -0.6 5,837,605 + BT4430 0.90 -0.3 5,837,650 - -1.2 5,837,666 - -2.4 5,837,726 - -2.2 5,837,851 + -1.9 5,837,869 + -1.9 5,837,913 - -1.1 5,838,006 - +0.4 5,838,109 + +1.0 5,838,119 + -1.0 5,838,175 - -1.4 5,838,175 - -1.1 5,838,246 + BT4431 0.11 -1.1 5,838,248 + BT4431 0.11 -1.8 5,838,324 + BT4431 0.15 +0.4 5,838,374 + BT4431 0.18 -0.6 5,838,375 - BT4431 0.18 -0.6 5,838,376 + BT4431 0.18 -0.1 5,838,376 + BT4431 0.18 -1.4 5,838,376 + BT4431 0.18 -0.6 5,838,377 - BT4431 0.18 -1.1 5,838,379 - BT4431 0.18 -0.6 5,838,381 - BT4431 0.18 -2.6 5,838,547 - BT4431 0.27 +0.4 5,838,635 + BT4431 0.32 -1.7 5,838,635 + BT4431 0.32 -1.1 5,838,720 - BT4431 0.37 -0.1 5,838,745 + BT4431 0.38 -0.6 5,838,763 + BT4431 0.39 -3.3 5,838,853 - BT4431 0.44 -3.0 5,838,971 + BT4431 0.51 -1.9 5,838,972 - BT4431 0.51 -1.1 5,839,015 + BT4431 0.53 +1.1 5,839,015 + BT4431 0.53 -1.1 5,839,016 - BT4431 0.53 +0.4 5,839,016 - BT4431 0.53 +0.4 5,839,116 - BT4431 0.59 -0.6 5,839,116 - BT4431 0.59 -1.6 5,839,154 - BT4431 0.61 -0.6 5,839,154 - BT4431 0.61 +0.4 5,839,195 - BT4431 0.63 -1.7 5,839,196 - BT4431 0.63 -2.1 5,839,209 + BT4431 0.64 -2.4 5,839,209 + BT4431 0.64 +1.4 5,839,269 + BT4431 0.67 -1.3 5,839,493 + BT4431 0.80 -1.6 5,839,493 + BT4431 0.80 -1.5 5,839,494 - BT4431 0.80 -0.6 5,839,497 + BT4431 0.80 -1.6 5,839,498 - BT4431 0.80 -1.1 5,839,498 - BT4431 0.80 -0.7 5,839,498 - BT4431 0.80 -0.6 5,839,504 - BT4431 0.81 -1.1 5,839,529 - BT4431 0.82 -0.2 5,839,624 - BT4431 0.87 -2.4 5,839,676 - -0.1 5,839,693 - -0.4 5,840,133 - BT4432 0.66 -2.4 5,840,160 - BT4432 0.73 -1.6 5,840,160 - BT4432 0.73 -1.8 5,840,222 - BT4432 0.88 +0.2 5,840,222 - BT4432 0.88 +2.0 5,840,222 - BT4432 0.88 -2.4 5,840,265 - +1.4 5,840,276 - +0.4 5,840,346 - -0.7 5,840,426 - -1.6 5,840,454 - -1.2 5,840,509 - +0.4 5,840,650 - +0.4 5,840,680 - -1.7 5,840,680 - -1.4 5,840,683 - -1.6 5,840,719 - -0.2
Or see this region's nucleotide sequence