Experiment: Gly-Glu (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2542 and Pf6N2E2_2543 are separated by 3 nucleotides Pf6N2E2_2543 and Pf6N2E2_2544 are separated by 79 nucleotides
Pf6N2E2_2542 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 3,002,566 to 3,004,338
_2542
Pf6N2E2_2543 - Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30), at 3,004,342 to 3,006,087
_2543
Pf6N2E2_2544 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 3,006,167 to 3,007,357
_2544
Position (kb)
3004
3005
3006
3007 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3003.359 kb on + strand, within Pf6N2E2_2542 at 3003.360 kb on - strand, within Pf6N2E2_2542 at 3003.360 kb on - strand, within Pf6N2E2_2542 at 3003.419 kb on + strand, within Pf6N2E2_2542 at 3003.511 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.625 kb on + strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.626 kb on - strand, within Pf6N2E2_2542 at 3003.629 kb on + strand, within Pf6N2E2_2542 at 3003.629 kb on + strand, within Pf6N2E2_2542 at 3003.630 kb on - strand, within Pf6N2E2_2542 at 3003.630 kb on - strand, within Pf6N2E2_2542 at 3003.787 kb on + strand, within Pf6N2E2_2542 at 3003.788 kb on - strand, within Pf6N2E2_2542 at 3003.788 kb on - strand, within Pf6N2E2_2542 at 3003.788 kb on - strand, within Pf6N2E2_2542 at 3003.884 kb on + strand, within Pf6N2E2_2542 at 3004.064 kb on + strand, within Pf6N2E2_2542 at 3004.065 kb on - strand, within Pf6N2E2_2542 at 3004.065 kb on - strand, within Pf6N2E2_2542 at 3004.123 kb on + strand, within Pf6N2E2_2542 at 3004.124 kb on - strand, within Pf6N2E2_2542 at 3004.399 kb on + strand at 3004.399 kb on + strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.400 kb on - strand at 3004.568 kb on + strand, within Pf6N2E2_2543 at 3004.569 kb on - strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.570 kb on + strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.571 kb on - strand, within Pf6N2E2_2543 at 3004.680 kb on - strand, within Pf6N2E2_2543 at 3004.744 kb on + strand, within Pf6N2E2_2543 at 3004.744 kb on + strand, within Pf6N2E2_2543 at 3004.745 kb on - strand, within Pf6N2E2_2543 at 3004.745 kb on - strand, within Pf6N2E2_2543 at 3005.011 kb on + strand, within Pf6N2E2_2543 at 3005.011 kb on + strand, within Pf6N2E2_2543 at 3005.012 kb on - strand, within Pf6N2E2_2543 at 3005.012 kb on - strand, within Pf6N2E2_2543 at 3005.213 kb on + strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.214 kb on - strand, within Pf6N2E2_2543 at 3005.219 kb on + strand, within Pf6N2E2_2543 at 3005.220 kb on - strand, within Pf6N2E2_2543 at 3005.727 kb on + strand, within Pf6N2E2_2543 at 3005.727 kb on + strand, within Pf6N2E2_2543 at 3005.782 kb on + strand, within Pf6N2E2_2543 at 3005.977 kb on + strand at 3005.978 kb on - strand at 3006.086 kb on - strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.145 kb on + strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.146 kb on - strand at 3006.201 kb on - strand at 3006.201 kb on - strand at 3006.272 kb on + strand at 3006.272 kb on + strand at 3006.273 kb on - strand at 3006.623 kb on + strand, within Pf6N2E2_2544 at 3006.674 kb on + strand, within Pf6N2E2_2544 at 3006.674 kb on + strand, within Pf6N2E2_2544 at 3006.675 kb on - strand, within Pf6N2E2_2544 at 3006.675 kb on - strand, within Pf6N2E2_2544 at 3006.675 kb on - strand, within Pf6N2E2_2544 at 3006.830 kb on - strand, within Pf6N2E2_2544 at 3006.981 kb on - strand, within Pf6N2E2_2544 at 3007.058 kb on + strand, within Pf6N2E2_2544 at 3007.059 kb on - strand, within Pf6N2E2_2544 at 3007.061 kb on + strand, within Pf6N2E2_2544 at 3007.062 kb on - strand, within Pf6N2E2_2544 at 3007.062 kb on - strand, within Pf6N2E2_2544
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (N) remove 3,003,359 + Pf6N2E2_2542 0.45 +0.0 3,003,360 - Pf6N2E2_2542 0.45 -1.1 3,003,360 - Pf6N2E2_2542 0.45 +0.8 3,003,419 + Pf6N2E2_2542 0.48 +0.7 3,003,511 + Pf6N2E2_2542 0.53 +1.5 3,003,625 + Pf6N2E2_2542 0.60 -0.6 3,003,625 + Pf6N2E2_2542 0.60 +0.5 3,003,625 + Pf6N2E2_2542 0.60 -0.1 3,003,625 + Pf6N2E2_2542 0.60 -0.5 3,003,625 + Pf6N2E2_2542 0.60 +0.5 3,003,625 + Pf6N2E2_2542 0.60 -2.0 3,003,625 + Pf6N2E2_2542 0.60 +0.1 3,003,626 - Pf6N2E2_2542 0.60 -0.1 3,003,626 - Pf6N2E2_2542 0.60 +0.5 3,003,626 - Pf6N2E2_2542 0.60 +0.4 3,003,626 - Pf6N2E2_2542 0.60 +0.4 3,003,626 - Pf6N2E2_2542 0.60 +0.6 3,003,626 - Pf6N2E2_2542 0.60 +1.1 3,003,626 - Pf6N2E2_2542 0.60 -1.9 3,003,626 - Pf6N2E2_2542 0.60 -1.1 3,003,626 - Pf6N2E2_2542 0.60 +0.7 3,003,629 + Pf6N2E2_2542 0.60 -0.7 3,003,629 + Pf6N2E2_2542 0.60 -0.3 3,003,630 - Pf6N2E2_2542 0.60 +1.0 3,003,630 - Pf6N2E2_2542 0.60 -1.0 3,003,787 + Pf6N2E2_2542 0.69 -0.5 3,003,788 - Pf6N2E2_2542 0.69 -2.1 3,003,788 - Pf6N2E2_2542 0.69 +0.7 3,003,788 - Pf6N2E2_2542 0.69 -0.9 3,003,884 + Pf6N2E2_2542 0.74 -2.1 3,004,064 + Pf6N2E2_2542 0.84 +2.9 3,004,065 - Pf6N2E2_2542 0.85 +1.5 3,004,065 - Pf6N2E2_2542 0.85 -0.1 3,004,123 + Pf6N2E2_2542 0.88 -0.6 3,004,124 - Pf6N2E2_2542 0.88 +1.3 3,004,399 + +1.7 3,004,399 + +1.0 3,004,400 - -0.9 3,004,400 - -0.5 3,004,400 - -0.4 3,004,400 - +0.1 3,004,400 - -0.4 3,004,400 - -1.2 3,004,400 - +0.3 3,004,568 + Pf6N2E2_2543 0.13 +0.6 3,004,569 - Pf6N2E2_2543 0.13 +1.4 3,004,570 + Pf6N2E2_2543 0.13 -1.9 3,004,570 + Pf6N2E2_2543 0.13 +0.0 3,004,570 + Pf6N2E2_2543 0.13 -0.5 3,004,570 + Pf6N2E2_2543 0.13 -0.1 3,004,570 + Pf6N2E2_2543 0.13 -0.5 3,004,570 + Pf6N2E2_2543 0.13 +0.2 3,004,570 + Pf6N2E2_2543 0.13 +0.9 3,004,570 + Pf6N2E2_2543 0.13 -1.0 3,004,570 + Pf6N2E2_2543 0.13 +0.0 3,004,571 - Pf6N2E2_2543 0.13 -1.4 3,004,571 - Pf6N2E2_2543 0.13 -0.0 3,004,571 - Pf6N2E2_2543 0.13 -0.3 3,004,571 - Pf6N2E2_2543 0.13 +0.5 3,004,571 - Pf6N2E2_2543 0.13 +0.3 3,004,571 - Pf6N2E2_2543 0.13 -2.2 3,004,571 - Pf6N2E2_2543 0.13 -1.7 3,004,571 - Pf6N2E2_2543 0.13 -1.4 3,004,571 - Pf6N2E2_2543 0.13 -0.4 3,004,571 - Pf6N2E2_2543 0.13 -1.1 3,004,680 - Pf6N2E2_2543 0.19 -1.3 3,004,744 + Pf6N2E2_2543 0.23 -1.3 3,004,744 + Pf6N2E2_2543 0.23 -0.8 3,004,745 - Pf6N2E2_2543 0.23 -0.3 3,004,745 - Pf6N2E2_2543 0.23 -0.1 3,005,011 + Pf6N2E2_2543 0.38 +0.5 3,005,011 + Pf6N2E2_2543 0.38 -0.2 3,005,012 - Pf6N2E2_2543 0.38 +0.2 3,005,012 - Pf6N2E2_2543 0.38 +0.2 3,005,213 + Pf6N2E2_2543 0.50 -0.7 3,005,214 - Pf6N2E2_2543 0.50 -1.1 3,005,214 - Pf6N2E2_2543 0.50 -0.9 3,005,214 - Pf6N2E2_2543 0.50 +0.5 3,005,214 - Pf6N2E2_2543 0.50 +0.5 3,005,219 + Pf6N2E2_2543 0.50 -1.5 3,005,220 - Pf6N2E2_2543 0.50 +0.9 3,005,727 + Pf6N2E2_2543 0.79 +0.2 3,005,727 + Pf6N2E2_2543 0.79 -1.3 3,005,782 + Pf6N2E2_2543 0.82 -2.0 3,005,977 + -2.4 3,005,978 - +0.3 3,006,086 - -0.2 3,006,145 + +0.0 3,006,145 + +0.7 3,006,145 + +0.2 3,006,145 + -2.1 3,006,145 + -1.9 3,006,145 + +1.2 3,006,146 - -0.8 3,006,146 - +0.1 3,006,146 - -0.7 3,006,146 - -0.9 3,006,146 - -0.8 3,006,146 - +0.5 3,006,146 - -0.3 3,006,201 - +0.1 3,006,201 - -0.9 3,006,272 + -0.8 3,006,272 + +0.6 3,006,273 - +0.6 3,006,623 + Pf6N2E2_2544 0.38 +0.6 3,006,674 + Pf6N2E2_2544 0.43 +0.6 3,006,674 + Pf6N2E2_2544 0.43 -0.9 3,006,675 - Pf6N2E2_2544 0.43 +0.6 3,006,675 - Pf6N2E2_2544 0.43 +1.3 3,006,675 - Pf6N2E2_2544 0.43 -0.1 3,006,830 - Pf6N2E2_2544 0.56 +1.7 3,006,981 - Pf6N2E2_2544 0.68 -1.3 3,007,058 + Pf6N2E2_2544 0.75 -0.3 3,007,059 - Pf6N2E2_2544 0.75 +0.6 3,007,061 + Pf6N2E2_2544 0.75 +1.1 3,007,062 - Pf6N2E2_2544 0.75 +0.0 3,007,062 - Pf6N2E2_2544 0.75 -1.5
Or see this region's nucleotide sequence