Experiment: Gly-Glu (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1465 and Pf6N2E2_1466 are separated by 429 nucleotides Pf6N2E2_1466 and Pf6N2E2_1467 are separated by 20 nucleotides Pf6N2E2_1467 and Pf6N2E2_1468 overlap by 4 nucleotides
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Pf6N2E2_1466 - NAD-dependent formate dehydrogenase delta subunit, at 1,714,633 to 1,714,830
_1466
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Pf6N2E2_1468 - NAD-dependent formate dehydrogenase beta subunit, at 1,717,724 to 1,719,283
_1468
Position (kb)
1714
1715
1716
1717
1718 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1713.915 kb on - strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.095 kb on - strand at 1714.448 kb on - strand at 1714.448 kb on - strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.460 kb on - strand at 1714.460 kb on - strand at 1714.487 kb on - strand at 1714.611 kb on - strand at 1714.624 kb on + strand at 1714.624 kb on + strand at 1714.635 kb on - strand at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.834 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.988 kb on - strand at 1714.988 kb on - strand at 1715.068 kb on + strand at 1715.069 kb on - strand at 1715.257 kb on + strand, within Pf6N2E2_1467 at 1715.258 kb on - strand, within Pf6N2E2_1467 at 1715.258 kb on - strand, within Pf6N2E2_1467 at 1715.361 kb on + strand, within Pf6N2E2_1467 at 1715.361 kb on + strand, within Pf6N2E2_1467 at 1715.518 kb on + strand, within Pf6N2E2_1467 at 1715.518 kb on + strand, within Pf6N2E2_1467 at 1715.519 kb on - strand, within Pf6N2E2_1467 at 1716.112 kb on + strand, within Pf6N2E2_1467 at 1716.112 kb on + strand, within Pf6N2E2_1467 at 1716.113 kb on - strand, within Pf6N2E2_1467 at 1716.113 kb on - strand, within Pf6N2E2_1467 at 1716.113 kb on - strand, within Pf6N2E2_1467 at 1716.115 kb on + strand, within Pf6N2E2_1467 at 1716.115 kb on + strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.142 kb on + strand, within Pf6N2E2_1467 at 1716.142 kb on + strand, within Pf6N2E2_1467 at 1716.143 kb on - strand, within Pf6N2E2_1467 at 1716.648 kb on + strand, within Pf6N2E2_1467 at 1716.667 kb on + strand, within Pf6N2E2_1467 at 1716.667 kb on + strand, within Pf6N2E2_1467 at 1716.668 kb on - strand, within Pf6N2E2_1467 at 1716.668 kb on - strand, within Pf6N2E2_1467 at 1716.725 kb on - strand, within Pf6N2E2_1467 at 1716.760 kb on + strand, within Pf6N2E2_1467 at 1716.979 kb on + strand, within Pf6N2E2_1467 at 1716.992 kb on + strand, within Pf6N2E2_1467 at 1717.393 kb on + strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.404 kb on + strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.830 kb on + strand at 1717.831 kb on - strand at 1718.085 kb on + strand, within Pf6N2E2_1468 at 1718.311 kb on - strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.483 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.594 kb on - strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.632 kb on - strand, within Pf6N2E2_1468
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (N) remove 1,713,915 - Pf6N2E2_1465 0.78 +1.4 1,713,976 + Pf6N2E2_1465 0.83 -0.7 1,713,976 + Pf6N2E2_1465 0.83 +0.7 1,713,976 + Pf6N2E2_1465 0.83 -0.8 1,713,976 + Pf6N2E2_1465 0.83 -0.4 1,714,000 - Pf6N2E2_1465 0.85 -1.2 1,714,000 - Pf6N2E2_1465 0.85 +0.5 1,714,074 + +0.8 1,714,074 + -0.1 1,714,074 + +0.9 1,714,075 - +0.1 1,714,075 - -0.4 1,714,075 - -2.1 1,714,075 - -0.8 1,714,079 + -1.0 1,714,079 + +2.1 1,714,079 + -0.3 1,714,080 - +0.5 1,714,080 - -1.2 1,714,080 - -1.2 1,714,080 - +0.0 1,714,094 + -0.7 1,714,094 + +0.9 1,714,094 + +0.3 1,714,095 - +0.5 1,714,448 - +0.6 1,714,448 - -0.7 1,714,459 + +1.4 1,714,459 + +0.3 1,714,459 + -1.2 1,714,460 - +1.0 1,714,460 - +0.5 1,714,487 - -0.3 1,714,611 - -0.0 1,714,624 + +1.3 1,714,624 + -1.4 1,714,635 - +0.1 1,714,785 + Pf6N2E2_1466 0.77 -0.1 1,714,785 + Pf6N2E2_1466 0.77 -0.8 1,714,785 + Pf6N2E2_1466 0.77 +0.8 1,714,785 + Pf6N2E2_1466 0.77 +0.2 1,714,785 + Pf6N2E2_1466 0.77 -0.1 1,714,785 + Pf6N2E2_1466 0.77 +0.9 1,714,786 - Pf6N2E2_1466 0.77 +0.3 1,714,786 - Pf6N2E2_1466 0.77 +1.6 1,714,786 - Pf6N2E2_1466 0.77 -0.6 1,714,786 - Pf6N2E2_1466 0.77 +0.0 1,714,786 - Pf6N2E2_1466 0.77 -0.6 1,714,786 - Pf6N2E2_1466 0.77 -0.6 1,714,786 - Pf6N2E2_1466 0.77 -0.6 1,714,809 - Pf6N2E2_1466 0.89 +0.9 1,714,809 - Pf6N2E2_1466 0.89 +0.3 1,714,809 - Pf6N2E2_1466 0.89 -0.7 1,714,834 + +0.6 1,714,987 + -0.2 1,714,987 + -2.1 1,714,987 + +0.4 1,714,987 + +0.4 1,714,988 - -0.4 1,714,988 - -0.1 1,715,068 + -1.2 1,715,069 - -0.7 1,715,257 + Pf6N2E2_1467 0.14 -1.7 1,715,258 - Pf6N2E2_1467 0.14 -0.9 1,715,258 - Pf6N2E2_1467 0.14 -1.0 1,715,361 + Pf6N2E2_1467 0.18 -0.3 1,715,361 + Pf6N2E2_1467 0.18 +1.5 1,715,518 + Pf6N2E2_1467 0.23 -0.7 1,715,518 + Pf6N2E2_1467 0.23 -0.7 1,715,519 - Pf6N2E2_1467 0.23 -0.7 1,716,112 + Pf6N2E2_1467 0.44 -0.7 1,716,112 + Pf6N2E2_1467 0.44 +0.4 1,716,113 - Pf6N2E2_1467 0.44 -0.1 1,716,113 - Pf6N2E2_1467 0.44 -0.4 1,716,113 - Pf6N2E2_1467 0.44 +1.6 1,716,115 + Pf6N2E2_1467 0.44 +0.4 1,716,115 + Pf6N2E2_1467 0.44 +0.0 1,716,116 - Pf6N2E2_1467 0.44 -0.1 1,716,116 - Pf6N2E2_1467 0.44 -1.4 1,716,116 - Pf6N2E2_1467 0.44 +0.0 1,716,116 - Pf6N2E2_1467 0.44 -1.2 1,716,116 - Pf6N2E2_1467 0.44 -0.3 1,716,116 - Pf6N2E2_1467 0.44 -0.7 1,716,142 + Pf6N2E2_1467 0.45 +0.1 1,716,142 + Pf6N2E2_1467 0.45 -0.6 1,716,143 - Pf6N2E2_1467 0.45 +1.4 1,716,648 + Pf6N2E2_1467 0.62 -0.1 1,716,667 + Pf6N2E2_1467 0.63 -0.4 1,716,667 + Pf6N2E2_1467 0.63 -1.7 1,716,668 - Pf6N2E2_1467 0.63 -0.3 1,716,668 - Pf6N2E2_1467 0.63 +0.1 1,716,725 - Pf6N2E2_1467 0.65 -1.1 1,716,760 + Pf6N2E2_1467 0.66 -1.0 1,716,979 + Pf6N2E2_1467 0.74 -0.9 1,716,992 + Pf6N2E2_1467 0.74 -0.1 1,717,393 + Pf6N2E2_1467 0.88 -0.4 1,717,394 - Pf6N2E2_1467 0.88 -0.1 1,717,394 - Pf6N2E2_1467 0.88 +1.6 1,717,404 + Pf6N2E2_1467 0.89 +0.3 1,717,405 - Pf6N2E2_1467 0.89 -1.5 1,717,405 - Pf6N2E2_1467 0.89 +0.6 1,717,405 - Pf6N2E2_1467 0.89 -0.7 1,717,830 + +0.8 1,717,831 - -0.4 1,718,085 + Pf6N2E2_1468 0.23 +0.4 1,718,311 - Pf6N2E2_1468 0.38 -0.3 1,718,482 + Pf6N2E2_1468 0.49 -0.2 1,718,482 + Pf6N2E2_1468 0.49 -0.4 1,718,483 - Pf6N2E2_1468 0.49 +0.8 1,718,485 - Pf6N2E2_1468 0.49 -0.0 1,718,485 - Pf6N2E2_1468 0.49 +1.3 1,718,485 - Pf6N2E2_1468 0.49 +0.7 1,718,594 - Pf6N2E2_1468 0.56 -0.7 1,718,625 + Pf6N2E2_1468 0.58 -2.2 1,718,625 + Pf6N2E2_1468 0.58 +1.6 1,718,625 + Pf6N2E2_1468 0.58 -0.2 1,718,625 + Pf6N2E2_1468 0.58 +0.3 1,718,625 + Pf6N2E2_1468 0.58 +0.6 1,718,626 - Pf6N2E2_1468 0.58 +0.4 1,718,626 - Pf6N2E2_1468 0.58 -0.2 1,718,626 - Pf6N2E2_1468 0.58 +0.9 1,718,626 - Pf6N2E2_1468 0.58 -0.1 1,718,631 + Pf6N2E2_1468 0.58 +0.2 1,718,631 + Pf6N2E2_1468 0.58 +0.4 1,718,631 + Pf6N2E2_1468 0.58 -1.7 1,718,632 - Pf6N2E2_1468 0.58 -2.1
Or see this region's nucleotide sequence