Experiment: Gly-Glu (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotides Pf6N2E2_1308 and Pf6N2E2_1309 are separated by 5 nucleotides Pf6N2E2_1309 and Pf6N2E2_1310 are separated by 13 nucleotides
Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148
_1307
Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915
_1308
Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381
_1309
Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426
_1310
Position (kb)
1542
1543
1544
1545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1541.976 kb on + strand, within Pf6N2E2_1307 at 1542.058 kb on - strand, within Pf6N2E2_1307 at 1542.112 kb on + strand at 1542.377 kb on - strand, within Pf6N2E2_1308 at 1542.421 kb on + strand, within Pf6N2E2_1308 at 1542.422 kb on - strand, within Pf6N2E2_1308 at 1542.422 kb on - strand, within Pf6N2E2_1308 at 1542.431 kb on - strand, within Pf6N2E2_1308 at 1542.545 kb on - strand, within Pf6N2E2_1308 at 1542.559 kb on + strand, within Pf6N2E2_1308 at 1542.560 kb on - strand, within Pf6N2E2_1308 at 1542.739 kb on + strand, within Pf6N2E2_1308 at 1542.739 kb on + strand, within Pf6N2E2_1308 at 1542.979 kb on - strand at 1543.308 kb on + strand, within Pf6N2E2_1309 at 1543.318 kb on - strand, within Pf6N2E2_1309 at 1543.360 kb on + strand, within Pf6N2E2_1309 at 1543.361 kb on - strand, within Pf6N2E2_1309 at 1543.501 kb on + strand, within Pf6N2E2_1309 at 1543.565 kb on - strand, within Pf6N2E2_1309 at 1543.908 kb on + strand, within Pf6N2E2_1309 at 1544.136 kb on + strand, within Pf6N2E2_1309 at 1544.151 kb on + strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.301 kb on + strand at 1544.362 kb on + strand at 1544.362 kb on + strand at 1544.389 kb on + strand at 1544.390 kb on - strand at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.842 kb on + strand, within Pf6N2E2_1310 at 1544.965 kb on + strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1545.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-Glu (N) remove 1,541,976 + Pf6N2E2_1307 0.74 -0.6 1,541,976 + Pf6N2E2_1307 0.74 +0.2 1,541,976 + Pf6N2E2_1307 0.74 +1.2 1,541,976 + Pf6N2E2_1307 0.74 -1.5 1,541,976 + Pf6N2E2_1307 0.74 -2.5 1,542,058 - Pf6N2E2_1307 0.87 -1.3 1,542,112 + -0.8 1,542,377 - Pf6N2E2_1308 0.30 -0.5 1,542,421 + Pf6N2E2_1308 0.36 +0.7 1,542,422 - Pf6N2E2_1308 0.36 +1.9 1,542,422 - Pf6N2E2_1308 0.36 +0.7 1,542,431 - Pf6N2E2_1308 0.37 -1.5 1,542,545 - Pf6N2E2_1308 0.52 -0.1 1,542,559 + Pf6N2E2_1308 0.54 -1.5 1,542,560 - Pf6N2E2_1308 0.54 -1.3 1,542,739 + Pf6N2E2_1308 0.77 -1.6 1,542,739 + Pf6N2E2_1308 0.77 -0.7 1,542,979 - +0.9 1,543,308 + Pf6N2E2_1309 0.26 -0.3 1,543,318 - Pf6N2E2_1309 0.27 +1.3 1,543,360 + Pf6N2E2_1309 0.30 +0.9 1,543,361 - Pf6N2E2_1309 0.30 -0.5 1,543,501 + Pf6N2E2_1309 0.40 -2.2 1,543,565 - Pf6N2E2_1309 0.44 +0.4 1,543,908 + Pf6N2E2_1309 0.68 +0.4 1,544,136 + Pf6N2E2_1309 0.83 -1.4 1,544,151 + Pf6N2E2_1309 0.84 +0.6 1,544,152 - Pf6N2E2_1309 0.84 -1.8 1,544,152 - Pf6N2E2_1309 0.84 +0.3 1,544,301 + +0.4 1,544,362 + +1.1 1,544,362 + +0.4 1,544,389 + +1.1 1,544,390 - -0.1 1,544,617 + Pf6N2E2_1310 0.22 -1.5 1,544,617 + Pf6N2E2_1310 0.22 -0.3 1,544,617 + Pf6N2E2_1310 0.22 +0.2 1,544,618 - Pf6N2E2_1310 0.22 +0.0 1,544,618 - Pf6N2E2_1310 0.22 +2.4 1,544,794 + Pf6N2E2_1310 0.39 +0.5 1,544,794 + Pf6N2E2_1310 0.39 +0.9 1,544,795 - Pf6N2E2_1310 0.39 -0.4 1,544,795 - Pf6N2E2_1310 0.39 -0.4 1,544,795 - Pf6N2E2_1310 0.39 +0.7 1,544,837 - Pf6N2E2_1310 0.43 +0.2 1,544,837 - Pf6N2E2_1310 0.43 -1.0 1,544,837 - Pf6N2E2_1310 0.43 +1.0 1,544,842 + Pf6N2E2_1310 0.43 -0.8 1,544,965 + Pf6N2E2_1310 0.55 +0.9 1,544,966 - Pf6N2E2_1310 0.55 +0.5 1,544,966 - Pf6N2E2_1310 0.55 +0.2 1,545,347 - +0.2
Or see this region's nucleotide sequence