Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_58 and Pf6N2E2_59 are separated by 120 nucleotides Pf6N2E2_59 and Pf6N2E2_60 are separated by 193 nucleotides
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Pf6N2E2_59 - 16 kDa heat shock protein A, at 97,966 to 98,418
_59
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Position (kb) 97
98
99 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 97.377 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.894 kb on - strand at 97.894 kb on - strand at 97.971 kb on + strand at 98.072 kb on - strand, within Pf6N2E2_59 at 98.072 kb on - strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.081 kb on - strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.228 kb on - strand, within Pf6N2E2_59 at 98.462 kb on + strand at 98.468 kb on + strand at 98.468 kb on + strand at 98.469 kb on - strand at 98.469 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.546 kb on + strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.614 kb on - strand at 98.664 kb on + strand at 98.664 kb on + strand at 98.665 kb on - strand at 98.673 kb on + strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 99.034 kb on + strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 97,377 + Pf6N2E2_58 0.51 -0.2 97,411 + Pf6N2E2_58 0.55 -1.4 97,411 + Pf6N2E2_58 0.55 -0.7 97,889 + +0.2 97,889 + -0.1 97,889 + -0.5 97,889 + +0.5 97,889 + +0.0 97,889 + -0.6 97,889 + -0.1 97,894 - -0.2 97,894 - +1.2 97,971 + -0.5 98,072 - Pf6N2E2_59 0.23 +0.8 98,072 - Pf6N2E2_59 0.23 -0.1 98,080 + Pf6N2E2_59 0.25 -0.2 98,080 + Pf6N2E2_59 0.25 -1.0 98,080 + Pf6N2E2_59 0.25 -0.5 98,080 + Pf6N2E2_59 0.25 +0.2 98,081 - Pf6N2E2_59 0.25 +0.3 98,227 + Pf6N2E2_59 0.58 +0.8 98,227 + Pf6N2E2_59 0.58 -0.3 98,228 - Pf6N2E2_59 0.58 -2.3 98,462 + -1.1 98,468 + +0.5 98,468 + +0.1 98,469 - +0.5 98,469 - +0.4 98,531 - +0.3 98,531 - -2.4 98,531 - -0.1 98,531 - -0.1 98,546 + +0.8 98,547 - +0.2 98,547 - +0.1 98,547 - +0.7 98,614 - -0.9 98,664 + -0.5 98,664 + -0.5 98,665 - -1.7 98,673 + -0.0 98,674 - +0.5 98,674 - -0.1 98,674 - +0.1 98,675 + -1.3 98,675 + +0.2 98,675 + +0.7 98,675 + -2.1 98,675 + +0.1 98,675 + -0.4 98,675 + -0.2 98,675 + -0.3 98,675 + -0.8 98,675 + -0.3 98,675 + -0.2 98,676 - +1.3 98,676 - +1.3 98,676 - +0.7 98,676 - -0.1 98,676 - -0.1 98,676 - +0.1 98,676 - -0.5 98,676 - +0.5 98,676 - -1.7 98,676 - -0.6 98,676 - -0.5 98,676 - -1.3 98,676 - +0.9 98,676 - +1.2 99,034 + Pf6N2E2_60 0.13 -0.5 99,035 - Pf6N2E2_60 0.13 -0.9 99,035 - Pf6N2E2_60 0.13 +0.1 99,035 - Pf6N2E2_60 0.13 -2.9
Or see this region's nucleotide sequence