Experiment: MoLS4 minimal media with L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2318 and DvMF_2319 are separated by 398 nucleotides DvMF_2319 and DvMF_R0067 are separated by 206 nucleotides DvMF_R0067 and DvMF_2320 are separated by 200 nucleotides
DvMF_2318: DvMF_2318 - 5-formyltetrahydrofolate cyclo-ligase (RefSeq), at 2,917,092 to 2,917,796
_2318
DvMF_2319: DvMF_2319 - phosphoesterase, Icc protein-like protein (RefSeq), at 2,918,195 to 2,918,872
_2319
DvMF_R0067: DvMF_R0067 - tRNA-Met (RefSeq), at 2,919,079 to 2,919,155
_R0067
DvMF_2320: DvMF_2320 - 6-phosphogluconate dehydrogenase NAD-binding (RefSeq), at 2,919,356 to 2,920,219
_2320
Position (kb)
2918
2919 Strain fitness (log2 ratio)
-2
-1
0
1 at 2917.301 kb on + strand, within DvMF_2318 at 2917.375 kb on + strand, within DvMF_2318 at 2917.390 kb on + strand, within DvMF_2318 at 2917.749 kb on + strand at 2918.411 kb on - strand, within DvMF_2319 at 2918.411 kb on - strand, within DvMF_2319 at 2918.483 kb on - strand, within DvMF_2319 at 2918.513 kb on - strand, within DvMF_2319 at 2918.650 kb on + strand, within DvMF_2319 at 2918.722 kb on + strand, within DvMF_2319 at 2918.757 kb on + strand, within DvMF_2319 at 2919.321 kb on - strand at 2919.332 kb on - strand at 2919.417 kb on - strand at 2919.418 kb on - strand at 2919.422 kb on + strand at 2919.783 kb on + strand, within DvMF_2320 at 2919.798 kb on + strand, within DvMF_2320
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media with L-Serine remove 2,917,301 + DvMF_2318 0.30 +1.0 2,917,375 + DvMF_2318 0.40 -0.6 2,917,390 + DvMF_2318 0.42 -0.2 2,917,749 + -0.6 2,918,411 - DvMF_2319 0.32 -1.7 2,918,411 - DvMF_2319 0.32 +0.3 2,918,483 - DvMF_2319 0.42 -1.7 2,918,513 - DvMF_2319 0.47 +0.2 2,918,650 + DvMF_2319 0.67 -1.9 2,918,722 + DvMF_2319 0.78 -0.5 2,918,757 + DvMF_2319 0.83 -0.5 2,919,321 - -0.6 2,919,332 - -0.7 2,919,417 - -0.2 2,919,418 - -0.2 2,919,422 + -0.5 2,919,783 + DvMF_2320 0.49 -0.5 2,919,798 + DvMF_2320 0.51 +0.6
Or see this region's nucleotide sequence