Experiment: Mc formate, casamino free, with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03560 and MMP_RS03565 are separated by 90 nucleotides MMP_RS03565 and MMP_RS03570 are separated by 36 nucleotides
MMP_RS03560: MMP_RS03560 - Na+/H+ antiporter NhaC family protein, at 666,725 to 668,302
_RS03560
MMP_RS03565: MMP_RS03565 - uracil phosphoribosyltransferase, at 668,393 to 669,088
_RS03565
MMP_RS03570: MMP_RS03570 - uracil-xanthine permease family protein, at 669,125 to 670,366
_RS03570
Position (kb)
668
669
670 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 667.466 kb on - strand, within MMP_RS03560 at 667.631 kb on + strand, within MMP_RS03560 at 667.662 kb on - strand, within MMP_RS03560 at 667.662 kb on - strand, within MMP_RS03560 at 667.663 kb on + strand, within MMP_RS03560 at 667.663 kb on + strand, within MMP_RS03560 at 667.663 kb on + strand, within MMP_RS03560 at 667.666 kb on - strand, within MMP_RS03560 at 667.666 kb on - strand, within MMP_RS03560 at 667.666 kb on - strand, within MMP_RS03560 at 667.666 kb on - strand, within MMP_RS03560 at 667.667 kb on + strand, within MMP_RS03560 at 667.667 kb on + strand, within MMP_RS03560 at 667.671 kb on - strand, within MMP_RS03560 at 667.671 kb on - strand, within MMP_RS03560 at 667.672 kb on + strand, within MMP_RS03560 at 667.672 kb on + strand, within MMP_RS03560 at 667.787 kb on - strand, within MMP_RS03560 at 667.796 kb on - strand, within MMP_RS03560 at 667.823 kb on + strand, within MMP_RS03560 at 667.824 kb on - strand, within MMP_RS03560 at 667.824 kb on - strand, within MMP_RS03560 at 667.824 kb on - strand, within MMP_RS03560 at 667.825 kb on + strand, within MMP_RS03560 at 668.057 kb on - strand, within MMP_RS03560 at 668.061 kb on + strand, within MMP_RS03560 at 668.121 kb on + strand, within MMP_RS03560 at 668.144 kb on + strand, within MMP_RS03560 at 668.147 kb on - strand at 668.152 kb on - strand at 668.173 kb on - strand at 668.193 kb on + strand at 668.193 kb on + strand at 668.229 kb on - strand at 668.285 kb on - strand at 668.286 kb on + strand at 668.289 kb on + strand at 668.302 kb on - strand at 668.302 kb on - strand at 668.307 kb on + strand at 668.307 kb on + strand at 668.308 kb on - strand at 668.309 kb on + strand at 668.329 kb on + strand at 668.354 kb on + strand at 668.354 kb on + strand at 668.356 kb on + strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.480 kb on - strand, within MMP_RS03565 at 668.480 kb on - strand, within MMP_RS03565 at 668.484 kb on - strand, within MMP_RS03565 at 668.494 kb on - strand, within MMP_RS03565 at 668.591 kb on - strand, within MMP_RS03565 at 668.648 kb on + strand, within MMP_RS03565 at 668.719 kb on + strand, within MMP_RS03565 at 668.729 kb on - strand, within MMP_RS03565 at 668.730 kb on + strand, within MMP_RS03565 at 668.734 kb on - strand, within MMP_RS03565 at 668.842 kb on - strand, within MMP_RS03565 at 668.845 kb on - strand, within MMP_RS03565 at 668.860 kb on - strand, within MMP_RS03565 at 669.032 kb on - strand at 669.084 kb on + strand at 669.088 kb on - strand at 669.114 kb on + strand at 669.119 kb on + strand at 669.279 kb on + strand, within MMP_RS03570 at 669.310 kb on - strand, within MMP_RS03570 at 669.364 kb on - strand, within MMP_RS03570 at 669.416 kb on + strand, within MMP_RS03570 at 669.443 kb on - strand, within MMP_RS03570 at 669.687 kb on + strand, within MMP_RS03570 at 669.798 kb on + strand, within MMP_RS03570 at 669.825 kb on + strand, within MMP_RS03570 at 669.867 kb on + strand, within MMP_RS03570 at 669.883 kb on - strand, within MMP_RS03570 at 669.884 kb on + strand, within MMP_RS03570 at 669.906 kb on + strand, within MMP_RS03570 at 669.914 kb on - strand, within MMP_RS03570 at 669.931 kb on - strand, within MMP_RS03570 at 669.945 kb on - strand, within MMP_RS03570 at 670.069 kb on - strand, within MMP_RS03570
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM Glycine remove 667,466 - MMP_RS03560 0.47 +0.7 667,631 + MMP_RS03560 0.57 -0.4 667,662 - MMP_RS03560 0.59 -0.7 667,662 - MMP_RS03560 0.59 +0.1 667,663 + MMP_RS03560 0.59 -0.2 667,663 + MMP_RS03560 0.59 +0.3 667,663 + MMP_RS03560 0.59 +0.5 667,666 - MMP_RS03560 0.60 -0.5 667,666 - MMP_RS03560 0.60 -0.9 667,666 - MMP_RS03560 0.60 +0.3 667,666 - MMP_RS03560 0.60 -2.1 667,667 + MMP_RS03560 0.60 -0.1 667,667 + MMP_RS03560 0.60 +0.2 667,671 - MMP_RS03560 0.60 +0.6 667,671 - MMP_RS03560 0.60 +0.7 667,672 + MMP_RS03560 0.60 +1.0 667,672 + MMP_RS03560 0.60 +0.3 667,787 - MMP_RS03560 0.67 -0.3 667,796 - MMP_RS03560 0.68 -0.4 667,823 + MMP_RS03560 0.70 +0.1 667,824 - MMP_RS03560 0.70 +0.6 667,824 - MMP_RS03560 0.70 -0.2 667,824 - MMP_RS03560 0.70 +0.5 667,825 + MMP_RS03560 0.70 +0.1 668,057 - MMP_RS03560 0.84 -1.4 668,061 + MMP_RS03560 0.85 +0.2 668,121 + MMP_RS03560 0.88 +1.2 668,144 + MMP_RS03560 0.90 +0.2 668,147 - -0.6 668,152 - -0.6 668,173 - -0.6 668,193 + +0.7 668,193 + +0.7 668,229 - -0.4 668,285 - -0.7 668,286 + -0.2 668,289 + -0.2 668,302 - +1.4 668,302 - -0.1 668,307 + +0.7 668,307 + +0.9 668,308 - +0.4 668,309 + +1.0 668,329 + -2.4 668,354 + -0.5 668,354 + -1.4 668,356 + -2.7 668,386 - -2.7 668,386 - -0.6 668,386 - +0.1 668,386 - -0.5 668,386 - -0.4 668,480 - MMP_RS03565 0.12 +0.4 668,480 - MMP_RS03565 0.12 -1.0 668,484 - MMP_RS03565 0.13 -0.0 668,494 - MMP_RS03565 0.15 -0.5 668,591 - MMP_RS03565 0.28 -0.8 668,648 + MMP_RS03565 0.37 -1.0 668,719 + MMP_RS03565 0.47 -0.2 668,729 - MMP_RS03565 0.48 -0.9 668,730 + MMP_RS03565 0.48 -3.1 668,734 - MMP_RS03565 0.49 -0.1 668,842 - MMP_RS03565 0.65 -0.2 668,845 - MMP_RS03565 0.65 -2.1 668,860 - MMP_RS03565 0.67 +0.3 669,032 - -0.1 669,084 + -0.8 669,088 - -0.4 669,114 + -0.6 669,119 + +0.5 669,279 + MMP_RS03570 0.12 -0.1 669,310 - MMP_RS03570 0.15 +0.5 669,364 - MMP_RS03570 0.19 -0.3 669,416 + MMP_RS03570 0.23 +0.7 669,443 - MMP_RS03570 0.26 -0.8 669,687 + MMP_RS03570 0.45 +0.0 669,798 + MMP_RS03570 0.54 +0.6 669,825 + MMP_RS03570 0.56 -0.4 669,867 + MMP_RS03570 0.60 -1.0 669,883 - MMP_RS03570 0.61 +0.1 669,884 + MMP_RS03570 0.61 -0.3 669,906 + MMP_RS03570 0.63 +0.1 669,914 - MMP_RS03570 0.64 +0.6 669,931 - MMP_RS03570 0.65 +0.7 669,945 - MMP_RS03570 0.66 +0.1 670,069 - MMP_RS03570 0.76 -2.5
Or see this region's nucleotide sequence