Experiment: Mc formate, casamino free, with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02965 and MMP_RS02970 are separated by 334 nucleotides MMP_RS02970 and MMP_RS02975 are separated by 150 nucleotides MMP_RS02975 and MMP_RS02980 are separated by 63 nucleotides MMP_RS02980 and MMP_RS02985 are separated by 19 nucleotides
MMP_RS02965: MMP_RS02965 - carboxymuconolactone decarboxylase family protein, at 562,048 to 562,482
_RS02965
MMP_RS02970: MMP_RS02970 - hypothetical protein, at 562,817 to 563,107
_RS02970
MMP_RS02975: MMP_RS02975 - DUF2124 domain-containing protein, at 563,258 to 563,740
_RS02975
MMP_RS02980: MMP_RS02980 - ATP-grasp domain-containing protein, at 563,804 to 564,712
_RS02980
MMP_RS02985: MMP_RS02985 - hypothetical protein, at 564,732 to 565,655
_RS02985
Position (kb) 563
564 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 562.483 kb on + strand at 562.509 kb on - strand at 562.515 kb on + strand at 562.559 kb on - strand at 562.567 kb on + strand at 562.568 kb on - strand at 562.568 kb on - strand at 562.568 kb on - strand at 562.576 kb on - strand at 562.577 kb on + strand at 562.584 kb on + strand at 562.622 kb on + strand at 562.634 kb on + strand at 562.683 kb on - strand at 562.860 kb on - strand, within MMP_RS02970 at 562.860 kb on - strand, within MMP_RS02970 at 562.918 kb on + strand, within MMP_RS02970 at 562.918 kb on + strand, within MMP_RS02970 at 562.921 kb on + strand, within MMP_RS02970 at 563.057 kb on - strand, within MMP_RS02970 at 563.057 kb on - strand, within MMP_RS02970 at 563.057 kb on - strand, within MMP_RS02970 at 563.058 kb on + strand, within MMP_RS02970 at 563.060 kb on + strand, within MMP_RS02970 at 563.060 kb on + strand, within MMP_RS02970 at 563.060 kb on + strand, within MMP_RS02970 at 563.064 kb on - strand, within MMP_RS02970 at 563.064 kb on - strand, within MMP_RS02970 at 563.065 kb on + strand, within MMP_RS02970 at 563.139 kb on - strand at 563.139 kb on - strand at 563.166 kb on + strand at 563.166 kb on + strand at 563.169 kb on - strand at 563.305 kb on - strand at 563.305 kb on - strand at 563.305 kb on - strand at 563.338 kb on - strand, within MMP_RS02975 at 563.367 kb on + strand, within MMP_RS02975 at 563.368 kb on - strand, within MMP_RS02975 at 563.525 kb on + strand, within MMP_RS02975 at 563.565 kb on + strand, within MMP_RS02975 at 563.566 kb on - strand, within MMP_RS02975 at 563.650 kb on + strand, within MMP_RS02975 at 563.772 kb on + strand at 563.772 kb on + strand at 563.776 kb on - strand at 563.776 kb on - strand at 563.887 kb on - strand at 564.120 kb on - strand at 564.213 kb on - strand at 564.422 kb on - strand at 564.495 kb on - strand at 564.495 kb on - strand at 564.512 kb on - strand at 564.537 kb on - strand at 564.553 kb on - strand at 564.569 kb on - strand at 564.569 kb on - strand at 564.588 kb on - strand at 564.680 kb on + strand at 564.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM Glycine remove 562,483 + +0.2 562,509 - -0.6 562,515 + +0.1 562,559 - +0.7 562,567 + +0.6 562,568 - +0.1 562,568 - +0.4 562,568 - -0.0 562,576 - +0.5 562,577 + -2.8 562,584 + -0.3 562,622 + +1.3 562,634 + -0.3 562,683 - -0.1 562,860 - MMP_RS02970 0.15 +0.9 562,860 - MMP_RS02970 0.15 -0.1 562,918 + MMP_RS02970 0.35 -0.1 562,918 + MMP_RS02970 0.35 +0.7 562,921 + MMP_RS02970 0.36 +0.9 563,057 - MMP_RS02970 0.82 +0.3 563,057 - MMP_RS02970 0.82 +0.5 563,057 - MMP_RS02970 0.82 +0.8 563,058 + MMP_RS02970 0.83 -0.6 563,060 + MMP_RS02970 0.84 +0.4 563,060 + MMP_RS02970 0.84 -0.4 563,060 + MMP_RS02970 0.84 +0.6 563,064 - MMP_RS02970 0.85 -0.3 563,064 - MMP_RS02970 0.85 -0.7 563,065 + MMP_RS02970 0.85 -0.4 563,139 - -2.7 563,139 - -2.7 563,166 + -0.7 563,166 + -0.4 563,169 - +1.1 563,305 - +0.2 563,305 - +0.4 563,305 - -0.2 563,338 - MMP_RS02975 0.17 -1.0 563,367 + MMP_RS02975 0.23 +0.2 563,368 - MMP_RS02975 0.23 -0.3 563,525 + MMP_RS02975 0.55 -2.3 563,565 + MMP_RS02975 0.64 -0.1 563,566 - MMP_RS02975 0.64 -0.4 563,650 + MMP_RS02975 0.81 -0.1 563,772 + -2.2 563,772 + -2.3 563,776 - -0.1 563,776 - +0.4 563,887 - -0.7 564,120 - +0.2 564,213 - -2.2 564,422 - -0.8 564,495 - -1.5 564,495 - -1.2 564,512 - -1.2 564,537 - -2.9 564,553 - -1.2 564,569 - -2.2 564,569 - -4.3 564,588 - -0.8 564,680 + -0.9 564,696 + -2.0
Or see this region's nucleotide sequence