Experiment: MoLS4 minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2144 and DvMF_2145 overlap by 4 nucleotides DvMF_2145 and DvMF_2146 are separated by 325 nucleotides DvMF_2146 and DvMF_2147 are separated by 468 nucleotides
DvMF_2144: DvMF_2144 - GCN5-related N-acetyltransferase (RefSeq), at 2,670,048 to 2,670,587
_2144
DvMF_2145: DvMF_2145 - Protein of unknown function DUF1778 (RefSeq), at 2,670,584 to 2,670,856
_2145
DvMF_2146: DvMF_2146 - delta-1-pyrroline-5-carboxylate dehydrogenase (RefSeq), at 2,671,182 to 2,674,223
_2146
DvMF_2147: DvMF_2147 - zinc resistance-associated protein (RefSeq), at 2,674,692 to 2,675,231
_2147
Position (kb)
2671
2672
2673
2674
2675 Strain fitness (log2 ratio)
-2
-1
0
1 at 2670.281 kb on + strand, within DvMF_2144 at 2670.416 kb on - strand, within DvMF_2144 at 2670.755 kb on + strand, within DvMF_2145 at 2671.187 kb on - strand at 2671.370 kb on + strand at 2671.456 kb on - strand at 2671.875 kb on - strand, within DvMF_2146 at 2672.386 kb on - strand, within DvMF_2146 at 2672.772 kb on - strand, within DvMF_2146 at 2672.947 kb on + strand, within DvMF_2146 at 2672.955 kb on - strand, within DvMF_2146 at 2672.993 kb on - strand, within DvMF_2146 at 2673.356 kb on - strand, within DvMF_2146 at 2673.372 kb on + strand, within DvMF_2146 at 2673.448 kb on - strand, within DvMF_2146 at 2673.478 kb on - strand, within DvMF_2146 at 2673.590 kb on + strand, within DvMF_2146 at 2673.637 kb on - strand, within DvMF_2146 at 2673.725 kb on + strand, within DvMF_2146 at 2673.832 kb on + strand, within DvMF_2146 at 2673.871 kb on - strand, within DvMF_2146 at 2673.884 kb on - strand, within DvMF_2146 at 2673.891 kb on - strand, within DvMF_2146 at 2674.115 kb on - strand at 2674.115 kb on - strand at 2674.123 kb on + strand at 2674.248 kb on + strand at 2674.429 kb on + strand at 2674.960 kb on + strand, within DvMF_2147 at 2675.010 kb on - strand, within DvMF_2147
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media remove 2,670,281 + DvMF_2144 0.43 -1.4 2,670,416 - DvMF_2144 0.68 +0.5 2,670,755 + DvMF_2145 0.63 +1.4 2,671,187 - -0.2 2,671,370 + -0.4 2,671,456 - +0.1 2,671,875 - DvMF_2146 0.23 -0.7 2,672,386 - DvMF_2146 0.40 -0.1 2,672,772 - DvMF_2146 0.52 -0.5 2,672,947 + DvMF_2146 0.58 +0.2 2,672,955 - DvMF_2146 0.58 -0.9 2,672,993 - DvMF_2146 0.60 -1.2 2,673,356 - DvMF_2146 0.71 -1.1 2,673,372 + DvMF_2146 0.72 -0.1 2,673,448 - DvMF_2146 0.74 -0.8 2,673,478 - DvMF_2146 0.75 +0.2 2,673,590 + DvMF_2146 0.79 -0.3 2,673,637 - DvMF_2146 0.81 -0.4 2,673,725 + DvMF_2146 0.84 -0.4 2,673,832 + DvMF_2146 0.87 -0.5 2,673,871 - DvMF_2146 0.88 -1.0 2,673,884 - DvMF_2146 0.89 -2.0 2,673,891 - DvMF_2146 0.89 -0.2 2,674,115 - -0.6 2,674,115 - -0.6 2,674,123 + -0.6 2,674,248 + -0.9 2,674,429 + -0.7 2,674,960 + DvMF_2147 0.50 -2.5 2,675,010 - DvMF_2147 0.59 +1.2
Or see this region's nucleotide sequence