Experiment: MoLS4 minimal media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0321 and DvMF_0322 are separated by 279 nucleotides DvMF_0322 and DvMF_0323 overlap by 4 nucleotides DvMF_0323 and DvMF_0324 overlap by 4 nucleotides
DvMF_0321: DvMF_0321 - iron-containing alcohol dehydrogenase (RefSeq), at 374,055 to 375,236
_0321
DvMF_0322: DvMF_0322 - heterodisulfide reductase, C subunit (RefSeq), at 375,516 to 376,076
_0322
DvMF_0323: DvMF_0323 - CoB--CoM heterodisulfide reductase (RefSeq), at 376,073 to 377,053
_0323
DvMF_0324: DvMF_0324 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 377,050 to 379,008
_0324
Position (kb) 375
376
377 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 374.657 kb on - strand, within DvMF_0321 at 374.657 kb on - strand, within DvMF_0321 at 374.708 kb on - strand, within DvMF_0321 at 374.720 kb on + strand, within DvMF_0321 at 374.810 kb on + strand, within DvMF_0321 at 374.855 kb on + strand, within DvMF_0321 at 375.058 kb on + strand, within DvMF_0321 at 375.580 kb on + strand, within DvMF_0322 at 375.713 kb on - strand, within DvMF_0322 at 376.676 kb on - strand, within DvMF_0323 at 376.828 kb on - strand, within DvMF_0323 at 376.981 kb on + strand at 377.019 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 minimal media remove 374,657 - DvMF_0321 0.51 -0.7 374,657 - DvMF_0321 0.51 -0.1 374,708 - DvMF_0321 0.55 -0.2 374,720 + DvMF_0321 0.56 +0.1 374,810 + DvMF_0321 0.64 -0.9 374,855 + DvMF_0321 0.68 -0.7 375,058 + DvMF_0321 0.85 -0.4 375,580 + DvMF_0322 0.11 +0.2 375,713 - DvMF_0322 0.35 +2.8 376,676 - DvMF_0323 0.61 +1.4 376,828 - DvMF_0323 0.77 -0.3 376,981 + +0.3 377,019 - -3.4
Or see this region's nucleotide sequence