Experiment: Mc formate, casamino free, with 10mM Glycine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07395 and MMP_RS09050 are separated by 487 nucleotides MMP_RS09050 and MMP_RS09055 are separated by 267 nucleotides MMP_RS09055 and MMP_RS07405 are separated by 151 nucleotides
MMP_RS07395: MMP_RS07395 - DNA topoisomerase IV subunit A, at 1,407,876 to 1,408,973
_RS07395
MMP_RS09050: MMP_RS09050 - CAP domain-containing protein, at 1,409,461 to 1,409,646
_RS09050
MMP_RS09055: MMP_RS09055 - CAP domain-containing protein, at 1,409,914 to 1,410,327
_RS09055
MMP_RS07405: MMP_RS07405 - 2 3-bisphosphoglycerate-independent phosphoglycerate mutase, at 1,410,479 to 1,411,699
_RS07405
Position (kb)
1409
1410
1411 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1408.994 kb on + strand at 1409.049 kb on + strand at 1409.331 kb on + strand at 1409.388 kb on - strand at 1409.388 kb on - strand at 1409.395 kb on - strand at 1409.395 kb on - strand at 1409.396 kb on + strand at 1409.447 kb on + strand at 1409.460 kb on - strand at 1409.485 kb on + strand, within MMP_RS09050 at 1409.508 kb on - strand, within MMP_RS09050 at 1409.532 kb on - strand, within MMP_RS09050 at 1409.703 kb on - strand at 1409.777 kb on - strand at 1409.868 kb on + strand at 1409.895 kb on - strand at 1409.895 kb on - strand at 1409.896 kb on + strand at 1409.896 kb on + strand at 1409.896 kb on + strand at 1409.907 kb on - strand at 1409.907 kb on - strand at 1409.908 kb on + strand at 1409.908 kb on + strand at 1409.908 kb on + strand at 1409.911 kb on + strand at 1409.949 kb on + strand at 1409.949 kb on + strand at 1410.024 kb on + strand, within MMP_RS09055 at 1410.298 kb on - strand at 1410.299 kb on + strand at 1410.299 kb on + strand at 1410.302 kb on + strand at 1410.343 kb on + strand at 1410.379 kb on + strand at 1410.479 kb on + strand at 1410.479 kb on + strand at 1410.501 kb on - strand at 1410.502 kb on + strand at 1410.788 kb on + strand at 1410.793 kb on + strand at 1411.131 kb on - strand at 1411.131 kb on - strand at 1411.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mc formate, casamino free, with 10mM Glycine remove 1,408,994 + -0.6 1,409,049 + +0.6 1,409,331 + +0.3 1,409,388 - +0.5 1,409,388 - -1.2 1,409,395 - -1.8 1,409,395 - +0.2 1,409,396 + +0.3 1,409,447 + -0.5 1,409,460 - -0.1 1,409,485 + MMP_RS09050 0.13 +0.6 1,409,508 - MMP_RS09050 0.25 -0.1 1,409,532 - MMP_RS09050 0.38 -0.1 1,409,703 - +0.2 1,409,777 - +0.8 1,409,868 + +1.2 1,409,895 - -0.3 1,409,895 - -0.9 1,409,896 + -2.2 1,409,896 + +0.9 1,409,896 + +0.1 1,409,907 - +0.3 1,409,907 - -0.8 1,409,908 + +1.4 1,409,908 + -1.9 1,409,908 + -0.4 1,409,911 + -0.1 1,409,949 + +0.7 1,409,949 + -0.1 1,410,024 + MMP_RS09055 0.27 -1.7 1,410,298 - +1.7 1,410,299 + +0.9 1,410,299 + -1.0 1,410,302 + +1.1 1,410,343 + -0.4 1,410,379 + +0.5 1,410,479 + -2.9 1,410,479 + -1.2 1,410,501 - -1.2 1,410,502 + -0.5 1,410,788 + -2.2 1,410,793 + -1.3 1,411,131 - +1.3 1,411,131 - -1.1 1,411,131 - -0.1
Or see this region's nucleotide sequence